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While studying blue light-independent effects of cryptochrome 1 (cry1) photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein α subunit (GPA1) null mutant gpa1. Both cry1 and gpa1 also showed reduced accumulation of anthocyanin under blue light. These convergent gpa1 and cry1 phenotypes required the presence of sucrose in the growth media and were not additive in the cry1gpa1 double mutant, suggesting context-dependent signaling convergence between cry1 and GPA1 signaling pathways. Both, gpa1 and cry1 mutants showed reduced GTP-binding activity. The cry1 mutant showed wild-type levels of GPA1 mRNA or GPA1 protein. However, an anti-transducin antibody (AS/7) typically used for plant Gα proteins, recognized a 54 kDa band in the wild type but not in gpa1 and cry1 mutants. We propose a model where cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity. © 2012 Springer Science+Business Media B.V.


Documento: Artículo
Título:Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis
Autor:Fox, A.R.; Soto, G.C.; Jones, A.M.; Casal, J.J.; Muschietti, J.P.; Mazzella, M.A.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Dr. Hector Torres, (INGEBI-CONICET), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Instituto de Genética Ewald A. Favret, CICVyA, INTA Castelar, 1712 Buenos Aires, Argentina
Departments of Biology and Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
IFEVA, Facultad de Agronomía, Universidad de Buenos Aires and CONICET, 1417 Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Fundacion Instituto Leloir, 1405 Buenos Aires, Argentina
Palabras clave:Arabidopsis thaliana; cry1; GPA1; Heterotrimeric G-proteins; Signal transduction; anthocyanin; Arabidopsis protein; CRY1 protein, Arabidopsis; cryptochrome; GPA1 protein, Arabidopsis; guanine nucleotide binding protein alpha subunit; messenger RNA; plant RNA; sucrose; Arabidopsis; article; biological model; biosynthesis; drug effect; gene expression regulation; genetics; light; metabolism; mutation; phenotype; physiology; plant growth; protein binding; protein processing; radiation exposure; seedling; signal transduction; Anthocyanins; Arabidopsis; Arabidopsis Proteins; Cryptochromes; Gene Expression Regulation, Plant; GTP-Binding Protein alpha Subunits; Hypocotyl; Light; Models, Biological; Mutation; Phenotype; Protein Binding; Protein Processing, Post-Translational; RNA, Messenger; RNA, Plant; Seedling; Signal Transduction; Sucrose; Arabidopsis; Arabidopsis thaliana
Página de inicio:315
Página de fin:324
Título revista:Plant Molecular Biology
Título revista abreviado:Plant. Mol. Biol.
CAS:cryptochrome, 73745-06-9; sucrose, 122880-25-5, 57-50-1; Anthocyanins; Arabidopsis Proteins; CRY1 protein, Arabidopsis; Cryptochromes; GPA1 protein, Arabidopsis; GTP-Binding Protein alpha Subunits; RNA, Messenger; RNA, Plant; Sucrose, 57-50-1


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---------- APA ----------
Fox, A.R., Soto, G.C., Jones, A.M., Casal, J.J., Muschietti, J.P. & Mazzella, M.A. (2012) . Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis. Plant Molecular Biology, 80(3), 315-324.
---------- CHICAGO ----------
Fox, A.R., Soto, G.C., Jones, A.M., Casal, J.J., Muschietti, J.P., Mazzella, M.A. "Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis" . Plant Molecular Biology 80, no. 3 (2012) : 315-324.
---------- MLA ----------
Fox, A.R., Soto, G.C., Jones, A.M., Casal, J.J., Muschietti, J.P., Mazzella, M.A. "Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis" . Plant Molecular Biology, vol. 80, no. 3, 2012, pp. 315-324.
---------- VANCOUVER ----------
Fox, A.R., Soto, G.C., Jones, A.M., Casal, J.J., Muschietti, J.P., Mazzella, M.A. Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis. Plant. Mol. Biol. 2012;80(3):315-324.