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Abstract:

The plant plasma membrane barrier can express aquaporins (PIP1 and PIP2) that show two intriguing aspects: (1) the potential of modulating whole membrane water permeability by co-expression of both types, which have recently been distinguished for showing a different capacity to reach the plasma membrane; and (2) the faculty to reduce water permeation through the pore after cytosolic acidification, as a consequence of a gating process. Our working hypothesis is that these two key features might enhance plasticity of the membrane water transport capacity if they jointly trigger any cooperative interaction. In previous work, we proved by biophysical approaches that the plasma membrane of the halophyte Beta vulgaris storage root presents highly permeable aquaporins that can be shut down by acidic pH. Root Beta vulgaris PIPs were therefore subcloned and expressed in Xenopus oocytes. Co-expression of BvPIP1;1 and BvPIP2;2 not only enhances oocyte plasma membrane water permeability synergistically but also reinforces pH inhibitory response from partial to complete shut down after cytosolic pH acidification. This pH dependent behavior shows that PIP1-PIP2 co-expression accounts for a different pH sensitivity in comparison with PIP2 expression. These results prove for the first time that PIP co-expression modulates the membrane water permeability through a pH regulatory response, enhancing in this way membrane versatility to adjust its water transfer capacity. © 2010 Springer Science+Business Media B.V.

Registro:

Documento: Artículo
Título:Intracellular pH sensing is altered by plasma membrane PIP aquaporin co-expression
Autor:Bellati, J.; Alleva, K.; Soto, G.; Vitali, V.; Jozefkowicz, C.; Amodeo, G.
Filiación:Laboratorio de Biomembranas, Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, UBA, Pabellón II Piso 4 Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Palabras clave:Aquaporin gating; Cytosolic acidification; pH sensing; Plasma membrane intrinsic proteins; aquaporin; plant DNA; primer DNA; recombinant protein; vegetable protein; water; amino acid sequence; animal; article; beet; cell membrane permeability; female; gene expression; genetics; in vitro study; intracellular fluid; metabolism; molecular genetics; nucleotide sequence; oocyte; pH; phylogeny; plant gene; plant root; sequence homology; Xenopus laevis; Amino Acid Sequence; Animals; Aquaporins; Base Sequence; Beta vulgaris; Cell Membrane Permeability; DNA Primers; DNA, Plant; Female; Gene Expression; Genes, Plant; Hydrogen-Ion Concentration; Intracellular Fluid; Molecular Sequence Data; Oocytes; Phylogeny; Plant Proteins; Plant Roots; Recombinant Proteins; Sequence Homology, Amino Acid; Water; Xenopus laevis; Beta vulgaris; Beta vulgaris subsp. vulgaris; Pips
Año:2010
Volumen:74
Número:1
Página de inicio:105
Página de fin:118
DOI: http://dx.doi.org/10.1007/s11103-010-9658-8
Título revista:Plant Molecular Biology
Título revista abreviado:Plant. Mol. Biol.
ISSN:01674412
CODEN:PMBID
CAS:aquaporin, 215587-75-0; water, 7732-18-5; Aquaporins; DNA Primers; DNA, Plant; Plant Proteins; Recombinant Proteins; Water, 7732-18-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674412_v74_n1_p105_Bellati

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Citas:

---------- APA ----------
Bellati, J., Alleva, K., Soto, G., Vitali, V., Jozefkowicz, C. & Amodeo, G. (2010) . Intracellular pH sensing is altered by plasma membrane PIP aquaporin co-expression. Plant Molecular Biology, 74(1), 105-118.
http://dx.doi.org/10.1007/s11103-010-9658-8
---------- CHICAGO ----------
Bellati, J., Alleva, K., Soto, G., Vitali, V., Jozefkowicz, C., Amodeo, G. "Intracellular pH sensing is altered by plasma membrane PIP aquaporin co-expression" . Plant Molecular Biology 74, no. 1 (2010) : 105-118.
http://dx.doi.org/10.1007/s11103-010-9658-8
---------- MLA ----------
Bellati, J., Alleva, K., Soto, G., Vitali, V., Jozefkowicz, C., Amodeo, G. "Intracellular pH sensing is altered by plasma membrane PIP aquaporin co-expression" . Plant Molecular Biology, vol. 74, no. 1, 2010, pp. 105-118.
http://dx.doi.org/10.1007/s11103-010-9658-8
---------- VANCOUVER ----------
Bellati, J., Alleva, K., Soto, G., Vitali, V., Jozefkowicz, C., Amodeo, G. Intracellular pH sensing is altered by plasma membrane PIP aquaporin co-expression. Plant. Mol. Biol. 2010;74(1):105-118.
http://dx.doi.org/10.1007/s11103-010-9658-8