Artículo

Frankel, N.; Nunes-Nesi, A.; Balbo, I.; Mazuch, J.; Centeno, D.; Iusem, N.D.; Fernie, A.R.; Carrari, F. "ci21A/Asr1 expression influences glucose accumulation in potato tubers" (2007) Plant Molecular Biology. 63(5):719-730
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Abstract:

Asr genes are exclusively found in the genomes of higher plants. In many species, this gene family is expressed under abiotic stress conditions and during fruit ripening. The encoded proteins have nuclear localisation and consequently a transcription factor function has been suggested. Interestingly, yeast-one-hybrid experiments revealed that a grape ASR binds to the promoter of a hexose transporter gene (VvHT1). However, the role of these proteins in planta is still elusive. By using a reverse genetics approach in potato we found that modification of Asr1 expression has no incidence on the aerial phenotype of the plant but exerts a dramatic effect in tuber. Asr1 antisense potatoes displayed decreased tuber fresh weight whereas Asr1 overexpressors had a diminished number of tubers. Moreover, overexpression lines showed lower transcript levels of a plasma membrane hexose transporter and a concomitant decrease in glucose content in parenchyma cells of potato tubers. On the same hand glucose uptake rate was also reduced in one of the overexpressing lines. It thus seems likely that Asr1 is involved in the control of hexose uptake in heterotrophic organs. In addition, the transgenic plants were characterized by several other changes in steady state metabolite levels. Results presented here support a role for ci21A/Asr1 in glucose metabolism of potato tuber. © 2007 Springer Science+Business Media B.V.

Registro:

Documento: Artículo
Título:ci21A/Asr1 expression influences glucose accumulation in potato tubers
Autor:Frankel, N.; Nunes-Nesi, A.; Balbo, I.; Mazuch, J.; Centeno, D.; Iusem, N.D.; Fernie, A.R.; Carrari, F.
Filiación:Laboratorio de Fisiología Y Biología Molecular, IFIBYNE-Conicet, Universidad de Buenos Aires, Buenos Aires, Argentina
Max-Planck Institute for Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
Instituto de Biotecnología, Instituto Nacional de Tecnología Agrícola (IB-INTA), CICV-INTA, P.O. BOX 25, B1712WAA Castelar, Argentina
Palabras clave:Asr genes; Hexose metabolism; Potato tuber; Tomato; Asr genes; Hexose metabolism; Potato tuber; Binding energy; Biodiversity; Glucose; Plant cell culture; Plants (botany); Genes; Ci21A protein, Solanum tuberosum; glucose; heat shock protein; messenger RNA; primer DNA; unclassified drug; vegetable protein; article; gene expression regulation; genetics; metabolism; photosynthesis; plant; plant root; potato; reverse transcription polymerase chain reaction; transgenic plant; DNA Primers; Gene Expression Regulation, Plant; Glucose; Heat-Shock Proteins; Photosynthesis; Plant Components, Aerial; Plant Proteins; Plant Roots; Plants, Genetically Modified; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Solanum tuberosum; Binding; Cells; Genes; Glucose; Phenotypes; Plants; Tomatoes; Embryophyta; Lycopersicon esculentum; Solanum tuberosum; Tuber (truffle); Vitaceae
Año:2007
Volumen:63
Número:5
Página de inicio:719
Página de fin:730
DOI: http://dx.doi.org/10.1007/s11103-006-9120-0
Título revista:Plant Molecular Biology
Título revista abreviado:Plant. Mol. Biol.
ISSN:01674412
CODEN:PMBID
CAS:glucose, 50-99-7, 84778-64-3; Ci21A protein, Solanum tuberosum; DNA Primers; Glucose, 50-99-7; Heat-Shock Proteins; Plant Proteins; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674412_v63_n5_p719_Frankel

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Citas:

---------- APA ----------
Frankel, N., Nunes-Nesi, A., Balbo, I., Mazuch, J., Centeno, D., Iusem, N.D., Fernie, A.R.,..., Carrari, F. (2007) . ci21A/Asr1 expression influences glucose accumulation in potato tubers. Plant Molecular Biology, 63(5), 719-730.
http://dx.doi.org/10.1007/s11103-006-9120-0
---------- CHICAGO ----------
Frankel, N., Nunes-Nesi, A., Balbo, I., Mazuch, J., Centeno, D., Iusem, N.D., et al. "ci21A/Asr1 expression influences glucose accumulation in potato tubers" . Plant Molecular Biology 63, no. 5 (2007) : 719-730.
http://dx.doi.org/10.1007/s11103-006-9120-0
---------- MLA ----------
Frankel, N., Nunes-Nesi, A., Balbo, I., Mazuch, J., Centeno, D., Iusem, N.D., et al. "ci21A/Asr1 expression influences glucose accumulation in potato tubers" . Plant Molecular Biology, vol. 63, no. 5, 2007, pp. 719-730.
http://dx.doi.org/10.1007/s11103-006-9120-0
---------- VANCOUVER ----------
Frankel, N., Nunes-Nesi, A., Balbo, I., Mazuch, J., Centeno, D., Iusem, N.D., et al. ci21A/Asr1 expression influences glucose accumulation in potato tubers. Plant. Mol. Biol. 2007;63(5):719-730.
http://dx.doi.org/10.1007/s11103-006-9120-0