Artículo

Raíces, M.; Gargantini, P.R.; Chinchilla, D.; Crespi, M.; Téllez-Iñón, M.T.; Ulloa, R.M. "Regulation of CDPK isoforms during tuber development" (2003) Plant Molecular Biology. 52(5):1011-1024
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Abstract:

CDPK activities present during tuber development were analysed. A high CDPK activity was detected in the soluble fraction of early stolons and a lower one was detected in soluble and particulate fractions of induced stolons. The early and late CDPK activities displayed diverse specificity for in vitro substrates and different subcellular distribution. Western blot analysis revealed two CDPKs of 55 and 60 kDa that follow a precise spatial and temporal profile of expression. The 55 kDa protein was only detected in early-elongating stolons and the 60 kDa one was induced upon stolon swelling, correlating with early and late CDPK activities. A new member of the potato CDPK family, StCDPK3, was identified from a stolon cDNA library. Gene specific RT-PCR demonstrated that this gene is only expressed in early stolons, while the previously identified StCDPK1 is expressed upon stolon swelling. This expression profile suggests that StCDPK3 could correspond to the 55 kDa isoform while StCDPK1 could encode the 60 kDa isoform present in swelling stolons. StCDPK1 has myristoylation and palmitoylation consensus possibly involved in its dual intracellular localization. Transient expression studies with wild-type and mutated forms of StCDPK1 fused to GFP were used to show that subcellular localization of this isoform is controlled by myristoylation and palmitoylation. Altogether, our data suggest that sequential activation of StCDPK3 and StCDPK1 and the subcellular localisation of StCDPK1 might be critical regulatory steps of calcium signalling during potato tuber development.

Registro:

Documento: Artículo
Título:Regulation of CDPK isoforms during tuber development
Autor:Raíces, M.; Gargantini, P.R.; Chinchilla, D.; Crespi, M.; Téllez-Iñón, M.T.; Ulloa, R.M.
Filiación:Inst. Invest. Ing. Genet. Biol. M., CONICET Fac. de Cie. Exact. y Nat., Universidad de Buenos Aires, Vuelta de Obligado 2490 2do piso, 1428 Buenos Aires, Argentina
Inst. des Sci. Vég., Ctr. Natl. de la Rech. Scientifique, 1 Avenue de la Terrasse, F-91198, Gif sur Yvette, France
Palabras clave:Calcium-dependent protein kinases; Differential expression; Myristoylation; Palmitoylation; Solanum tuberosum; Tuberization; Genes; Plants (botany); Swelling; Particulate fractions; Molecular biology; Amino Acid Sequence; Base Sequence; Blotting, Western; Cell Membrane; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Green Fluorescent Proteins; Isoenzymes; Luminescent Proteins; Membrane Proteins; Microscopy, Confocal; Molecular Sequence Data; Myristic Acid; Palmitic Acids; Plant Proteins; Protein Kinases; Recombinant Fusion Proteins; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Solanum tuberosum; Substrate Specificity; Eukaryota; Solanum; Solanum tuberosum
Año:2003
Volumen:52
Número:5
Página de inicio:1011
Página de fin:1024
DOI: http://dx.doi.org/10.1023/A:1025478315648
Título revista:Plant Molecular Biology
Título revista abreviado:Plant. Mol. Biol.
ISSN:01674412
CODEN:PMBID
CAS:calcium-dependent protein kinases, plant, EC 2.7.1.-; Green Fluorescent Proteins, 147336-22-9; Isoenzymes; Luminescent Proteins; Membrane Proteins; Myristic Acid, 544-63-8; Palmitic Acids; Plant Proteins; Protein Kinases, EC 2.7.1.37; Recombinant Fusion Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674412_v52_n5_p1011_Raices

Referencias:

  • Ames, J.B., Ishima, R., Tanaka, T., Gordon, J.I., Stryer, L., Ikura, M., Molecular mechanics of calcium-myristoyl switches (1997) Nature, 389, pp. 198-202
  • Anil, V.S., Harmon, A.C., Rao, K.S., Spatio-temporal accumulation and activity of calcium-dependent kinases during embryogenesis, seed development, and germination in sandal-wood (2000) Plant Physiol., 122, pp. 1035-1043
  • Bachmann, M., Shiraishi, N., Campbell, W.H., Yoo, B.-C., Harmon, A.C., Huber, S.C., Identification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase (1996) Plant Cell, 8, pp. 505-517
  • Balamani, V., Veluthambi, K., Poovaiah, B.W., Effect of calcium on tuberization in potato (1986) Plant Physiol., 80, pp. 856-858
  • Bauer, P., Crespi, M.D., Szecsi, J., Allison, L.A., Schultze, M., Ratet, P., Kondorosi, E., Kondorosi, A., Alfalfa Enod12 genes are differentially regulated during nodule development by Nod factors and Rhizobium invasion (1994) Plant Physiol., 105, pp. 585-592
  • Botella, J.R., Arteca, J.M., Somodevilla, M., Arteca, R.N., Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata) (1996) Plant Mol. Biol., 30, pp. 1129-1137
  • Bradford, M.M., A rapid and sensitive method for the quantitation of micrograms of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254
  • Bush, D.S., Calcium regulation in plant cells and its role in signaling (1995) Annu. Rev. Plant Physiol. Plant Mol. Biol., 46, pp. 95-122
  • Calvert, P.D., Klenchin, V.A., Bownds, M.D., Rhodopsin kinase inhibition by recoverin. Function of recoverin myristoylation (1995) J. Biol. Chem., 270, pp. 24127-24129
  • Catchpole, A.H., Hillman, J., Effect of ethylene on tuber initiation in Solanum tuberosum L. (1969) Nature, 223, p. 1387
  • Cheng, S.H., Willmann, M.R., Chen, H.C., Sheen, J., Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family (2002) Plant Physiol., 129, pp. 469-485
  • Chico, J.M., Raices, M., Téllez-Iñón, M.T., Ulloa, R.M., A Calcium-dependent protein kinase is systemically induced upon wounding in tomato plants (2002) Plant Physiol., 128, pp. 256-270
  • Cutter, E.G., Structure and development of the potato plant (1978) The Growth of the Potato, pp. 99-113. , J.D. Ivins and F.L. Milthorpe (Eds.), Butterworth, London
  • Despres, B., Delseny, M., Devic, M., Partial complementation of embryo defective mutations: A general strategy to elucidate gene function (2001) Plant J., 27, pp. 149-159
  • Estruch, J.J., Kadwell, S., Merlin, E., Crossland, L., Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 8837-8841
  • Evans, N.H., McAinsh, M.R., Hetherington, A.M., Calcium oscillations in higher plants (2001) Curr. Opin. Plant Biol., 4, pp. 415-420
  • Ewing, E.E., Struik, P.C., Tuber formation in potato: Induction, initiation and growth (1992) Hort. Rev., 14, pp. 89-198
  • Godsel, L.M., Engman, D.M., Flagellar protein localization mediated by a calcium-myristoyl/palmitoyl switch mechanism (1999) EMBO J., 18, pp. 2057-2065
  • Grabski, S., Arnoys, E., Busch, B., Schindler, M., Regulation of actin tension in plant cells by kinases and phosphatases (1998) Plant Physiol., 116, pp. 279-290
  • Harmon, A.C., Putnam-Evans, C., Cormier, M.J., Calcium dependent but calmodulin independent protein kinase from soybean (1986) Plant Physiol., 83, pp. 830-837
  • Harmon, A.C., Gribskov, M., Harper, J.F., CDPKs: A kinase for every Ca2+ signal? (2000) Trends Plant. Sci., 5, pp. 154-159
  • Harmon, A.C., Gribskov, M., Gubrium, E., Harper, J.F., The CDPK superfamily of protein kinases (2001) New Phytol., 151, pp. 175-183
  • Harper, J.F., Sussman, M.R., Schaller, G.E., Putnam-Evans, C., Charbonneau, H., Harmon, A.C., A calcium-dependent protein kinase with a regulatory domain similar to calmodulin (1991) Science, 252, pp. 951-954
  • Hepler, P.K., Vidali, L., Cheung, A.Y., Polarized cell growth in higher plants (2001) Annu. Rev. Cell Dev. Biol., 17, pp. 159-187
  • Hrabak, E.M., Calcium-dependent protein kinases and their relatives (2000) Adv. Bot. Res., 32, pp. 185-223
  • Jackson, S.D., Multiple signaling pathways controls tuber induction in potato (1999) Plant Physiol., 119, pp. 1-8
  • Lee, J.-Y., Yoo, B.-C., Harmon, A.C., Kinetic and calcium-binding properties of three calcium-dependent protein kinase isoenzymes from soybean (1998) Biochemistry, 37, pp. 6801-6809
  • Lu, S.X., Hrabak, E.M., An Arabidopsis calcium-dependent protein kinase is associated with the endoplasmic reticulum (2002) Plant Physiol., 128, pp. 1008-1021
  • MacIntosh, G.C., Ulloa, R.M., Raices, M., Tellez-Iñón, M.T., Changes in calcium-dependent protein kinase activity in in vitro tuberization in potato (1996) Plant Physiol., 112, pp. 1541-1550
  • Martin, M.L., Busconi, L., Membrane localization of a rice calcium-dependent protein kinase (CDPK) is mediated by myristoylation and palmitoylation (2000) Plant J., 24, pp. 429-435
  • McCabe, J.B., Berthiaume, L.G., Functional roles for fatty acylated aminoterminal domains in subcellular localization (1999) Mol. Biol. Cell, 10, pp. 3771-3786
  • Mingo-Castel, A.M., Smith, O.E., Kumamoto, J., Studies on the carbon dioxide promotion and ethylene inhibition of tuberization in potato explants cultured in vitro (1976) Plant Physiol., 57, pp. 480-485
  • Mochly-Rosen, D., Localization of protein kinases by anchoring proteins: A theme in signal transduction (1995) Science, 268, pp. 247-251
  • Moutinho, A., Trewavas, A.J., Malhó, R., Relocation of a Ca2+-dependent protein kinase activity during tube reorientation (1998) Plant Cell, 10, pp. 1499-1509
  • Müller-Röber, B., Sonnewald, U., Willmitzer, L., Inhibition of the ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes (1992) EMBO J., 11, pp. 1229-1238
  • Nishiyama, R., Mizuno, H., Okada, S., Yamaguchi, T., Takenaka, M., Fukuzawa, H., Ohyama, K., Two mRNA species encoding calcium-dependent protein kinases are differentially expressed in sexual organs of Marchantia polymorpha through alternative splicing (1999) Plant Cell Physiol., 40, pp. 205-212
  • Park, W.D., Molecular approaches to tuberization in potato (1990) The Molecular and Cellular Biology of the Potato, pp. 43-56. , M.E. Vayda and W.D. Park (Eds.), C.A.B. International, Wallingford, CT
  • Pestenacz, A., Erdei, L., Calcium-dependent protein kinase in maize and sorghum induced by polyethylene glycol (1996) Physiol. Plant., 97, pp. 360-364
  • Poovaiah, B.W., Reddy, A.S.N., Calcium and signal transduction in plants (1993) Crit. Rev. Plant. Sci., 12, pp. 185-211
  • Prat, S., Frommer, W.B., Hofgen, R., Keil, M., Kossmann, J., Koster-Topfer, M., Liu, X.J., Willmitzer, L., Gene expression during tuber development in potato plants (1990) FEBS Lett., 268, pp. 334-338
  • Raíces, M., Chico, J.M., Téllez-Iñón, M.T., Ulloa, R.M., Molecular characterization of StCDPK1, a calcium-dependent protein kinase of Solanum tuberosum that is induced at the onset of tuber development (2001) Plant Mol. Biol., 46, pp. 591-601
  • Ritchie, S., Gilroy, S., Calcium-dependent protein phosphorylation may mediate the gibberellic acid response in barley aleurone (1998) Plant Physiol., 116, pp. 765-776
  • Roberts, D.M., Harmon, A.C., Calcium-modulated proteins: Targets of intracellular calcium signals in higher plants (1992) Annu. Rev. Plant Physiol. Plant Mol. Biol., 43, pp. 375-414
  • Romeis, T., Piedras, P., Jones, J.D.G., Resistance gene-dependent activation of a calcium-dependent protein kinase in the plant defense response (2000) Plant Cell, 12, pp. 803-815
  • Rutschmann, F., Stalder, U., Piotrowski, M., Oecking, C., Schaller, A., LeCPK1, a calcium-dependent protein kinase from tomato, plasma membrane targeting and biochemical characterization (2002) Plant Physiol., 129, pp. 156-168
  • Sanders, D., Pelloux, J., Brownlee, C., Harper, J.F., Calcium at the crossroads of signaling (2002) Plant Cell, 14, pp. 401-417
  • Scott, A., Wyatt, S., Tsou, P.-L., Robertson, D., Allen, N.S., Model system for plant cell biology: GFP imaging in living onion epidermal cells (1999) BioTechniques, 26, pp. 1125-1132
  • Sheen, J., Ca2+-dependent protein kinases and stress signal transduction in plants (1996) Science, 274, pp. 1900-1902
  • Shinozaki, K., Yamaguchi-Shinozaki, K., Gene expression and signal transduction in water-stress response (1997) Plant Physiol., 115, pp. 327-334
  • Short, J.M., Fernandez, J.M., Sorge, J.A., Huse, W.D., Lambda ZAP: A bacteriophage lambda expression vector with in vivo excision properties (1988) Nucl. Acids Res., 16, pp. 7583-7600
  • Sopory, S.K., Munshi, M., Protein kinases and phosphatases and their role in cellular signaling in plants (1998) Crit. Rev. Plant Sci., 17, pp. 245-318
  • Tanaka, T., Ames, J.B., Harvey, T.S., Stryer, L., Ikura, M., Sequestration of the membrane-targeting myristoyl group of recoverin in the calcium-free state (1995) Nature, 376, pp. 444-447
  • Taylor, M.A., George, L.A., Ross, H.A., Davies, H.V., cDNA cloning and characterisation of an α-glucosidase gene from potato (Solanum tuberosum L.) (1998) Plant J., 13, pp. 419-425
  • Thompson, G.A., Okuyama, H., Lipid-linked proteins of plants (2000) Prog. Lipid Res., 39, pp. 19-39
  • Ulloa, R.M., Raíces, M., MacIntosh, G., Téllez-Iñón, M.T., Jasmonic acid affects plant morphology and the expression and activity of a calcium-dependent protein kinase in Solanum tuberosum (2002) Physiol. Plant., 115, pp. 417-427
  • Visser, R.G.F., Vreugdenhil, D., Hendriks, T., Jacobsen, E.J., Gene expression and carbohydrate content during stolon to tuber transition in potatoes (Solanum tuberosum) (1994) Physiol. Plant., 90, pp. 285-292
  • Vreugdenhil, D., Struik, P.C., An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum) (1989) Physiol. Plant., 75, pp. 525-531
  • Yoon, G.M., Cho, H.S., Jung Ha, H., Liu, J.R., Lee, H.P., Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein (1999) Plant Mol. Biol., 39, pp. 991-1001
  • Zozulya, S., Stryer, L., Calcium-myristoyl protein switch (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 11569-11573

Citas:

---------- APA ----------
Raíces, M., Gargantini, P.R., Chinchilla, D., Crespi, M., Téllez-Iñón, M.T. & Ulloa, R.M. (2003) . Regulation of CDPK isoforms during tuber development. Plant Molecular Biology, 52(5), 1011-1024.
http://dx.doi.org/10.1023/A:1025478315648
---------- CHICAGO ----------
Raíces, M., Gargantini, P.R., Chinchilla, D., Crespi, M., Téllez-Iñón, M.T., Ulloa, R.M. "Regulation of CDPK isoforms during tuber development" . Plant Molecular Biology 52, no. 5 (2003) : 1011-1024.
http://dx.doi.org/10.1023/A:1025478315648
---------- MLA ----------
Raíces, M., Gargantini, P.R., Chinchilla, D., Crespi, M., Téllez-Iñón, M.T., Ulloa, R.M. "Regulation of CDPK isoforms during tuber development" . Plant Molecular Biology, vol. 52, no. 5, 2003, pp. 1011-1024.
http://dx.doi.org/10.1023/A:1025478315648
---------- VANCOUVER ----------
Raíces, M., Gargantini, P.R., Chinchilla, D., Crespi, M., Téllez-Iñón, M.T., Ulloa, R.M. Regulation of CDPK isoforms during tuber development. Plant. Mol. Biol. 2003;52(5):1011-1024.
http://dx.doi.org/10.1023/A:1025478315648