Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Amastigote forms of T. cruzi express the specific Ssp-4 surface antigen which is progressively shed, by the action of an endogenous phosphatidylinositol-phospholipase C, during their development into epimastigotes (Andrews et al., J. Exp. Med., 167 (1988) 300-314). We show now that the lipid moiety of the anchor of Ssp-4 is a ceramide which was metabolically labelled with [[3H]palmitic acid. The lipid could be cleaved by PI-PLC digestion in vitro, and was identified by methanolysis and reverse phase thin layer chromatography of the products, as palmitoyldihydrosphingosine. Also, the free biosynthesized lipids were investigated in parasites obtained after 0, 24, 48 and 72 h differentiation of trypomastigotes and further incubated with [3H]palmitic acid for 2 h. A maximum of free ceramide was found in the 24 h point, in accordance with the maximum of amastigote forms. In contrast only traces of free ceramide were found in trypomastigotes. The major ceramide (more than 90%) is palmitoyldihydrosphingosine, which is the same as found in the anchor of Ssp-4. The ceramide could play-an important role in the cell biology of the parasite as previously found for mammalian cells.

Registro:

Documento: Artículo
Título:Developmentally regulated expression of ceramide in Trypanosoma cruzi
Autor:Bertello, L.E.; Andrews, N.W.; De Lederkremer, R.M.
Filiación:Depto. de Quimica Orgánica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Cd. Universitaria, 1428 Buenos Aires, Argentina
Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520-8002, United States
Palabras clave:Ceramide; GPI-anchor; Ssp-4; Trypanosoma cruzi; ceramide; membrane antigen; n palmitoyldihydrosphingosine; palmitic acid; phosphatidylinositol; phospholipase C; tritium; unclassified drug; amastigote; article; controlled study; epimastigote; lipid analysis; nonhuman; priority journal; Trypanosoma cruzi; Mammalia; Tritium; Trypanosoma; Trypanosoma cruzi
Año:1996
Volumen:79
Número:2
Página de inicio:143
Página de fin:151
DOI: http://dx.doi.org/10.1016/0166-6851(96)02645-X
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:MOL. BIOCHEM. PARASITOL.
ISSN:01666851
CODEN:MBIPD
CAS:palmitic acid, 57-10-3; phospholipase C, 9001-86-9; tritium, 10028-17-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v79_n2_p143_Bertello

Referencias:

  • Andrews, N.W., Hong, K.S., Robbins, E., Nussenzweig, V., Stage-specific surface antigens expressed during the morphogenesis of vertebrate forms of Trypanosoma cruzi (1987) Exp. Parasitol., 64, pp. 474-484
  • Andrews, N.W., Robbins, E.S., Ley, V., Hong, K.S., Nussenzweig, V., Developmentally regulated, phospholipase C-mediated release of the major surface glycoprotein of amastigotes of Trypanosoma cruzi (1988) J. Exp. Med., 167, pp. 300-314
  • McConville, M.J., Ferguson, M.A.J., The structure, biosynthesis and function of glycosylated phosphatidylinositols in parasitic protozoa and higher eukaryotes (1993) Biochem. J., 294, pp. 305-324
  • Heise, N., Cardoso De Almeida, M.L., Ferguson, M.A.J., Characterization of the lipid moiety of the glycosylphosphatidyl inositol anchor of Trypanosoma cruzi 1G7-antigen (1995) Mol. Biochem. Parasitol., 70, pp. 71-84
  • Schenkman, S., Ferguson, M.A.J., Heise, N., Cardoso De Almeida, M.L., Mortara, R.A., Yoshida, N., Mucin-like glycoproteins linked to the membrane by glycosylphosphatidylinositol anchor are the major acceptors of sialic acid in a reaction catalyzed by trans-sialidase in metacyclic forms of Trypanosoma cruzi (1993) Mol. Biochem. Parasitol., 59, pp. 293-304
  • Couto, A.S., Lederkremer, R.M., Colli, W., Manso Alves, M.J., The glycosylphosphatidylinositol anchor of the trypomastigote-specific Tc-85 glycoprotein from Trypanosoma cruzi. Metabolic-labelling and structural studies (1993) Eur. J. Biochem., 217, pp. 597-602
  • Da Silveira, J.F., Colli, W., Chemical composition of the plasma membrane from epimastigote forms of Trypanosoma cruzi (1981) Biochim. Biophys. Acta, 644, pp. 341-350
  • Lederkremer, R.M., Lima, C., Ramirez, M.I., Ferguson, M.A.J., Homans, S.W., Thomas-Oates, J., Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes (1991) J. Biol. Chem., 266, pp. 23670-23675
  • Previato, J.O., Gorin, P.A.J., Mazurek, M., Xavier, M.T., Fournet, B., Wieruszesk, J.M., Mendonça-Previato, L., Primary structure of the oligosaccharide chain of lipopeptidophosphoglycan of epimastigote forms of Trypanosoma cruzi (1990) J. Biol. Chem., 265, pp. 2518-2526
  • Lederkremer, R.M., Lima, C., Ramirez, M.I., Casal, O.L., Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors (1990) Eur. J. Biochem., 192, pp. 337-345
  • Lederkremer, R.M., Lima, C., Ramirez, M.I., Gonçalves, M.F., Colli, W., Hexadecylpalmitoylglycerol or ceramide is linked to similar glycophosphoinositol anchor-like structures in Trypanosoma cruzi (1993) Eur. J. Biochem., 218, pp. 929-936
  • Serrano, A.A., Schenkman, S., Yoshida, N., Mehlert, A., Richardson, J.M., Ferguson, M.A.J., The lipid structure of the glycosylphosphatidylinositol-anchored mucin-like sialic acid acceptors of Trypanosoma cruzi changes during parasite differentiation from epimastigotes to infective metacyclic trypomastigote forms (1995) J. Biol. Chem., 270, pp. 27244-27253
  • Stadler, J., Keenan, T.W., Bauer, G., Gerish, G., The contact site a glycoprotein of Dictyostelium discoideum carries a phospholipid anchor of a novel type (1989) EMBO J., 8, pp. 371-377
  • Haynes, P.A., Gooley, A.A., Ferguson, M.A.J., Redmond, J.W., Williams, K.L., Post-translational modifications of the Dictyostelium discoideum glycoprotein PsA. Glycosylphosphatidylinositol membrane anchor and composition of O-linked oligosaccharides (1993) Eur. J. Biochem., 216, pp. 729-737
  • Conzelmann, A., Puoti, A., Lester, R.L., Desponds, C., Two different types of lipid moieties are present in glycosylphosphoinositol-anchored membrane proteins of Saccharomyces cerevisiae (1992) EMBO J., 11, pp. 457-466
  • Fankhauser, C., Homans, S.W., Thomas-Oates, J.E., McConville, M.J., Desponds, C., Conzelmann, A., Ferguson, M.A.J., Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae (1993) J. Biol. Chem., 268, pp. 26365-26374
  • Hannun, Y.A., The sphingomyelin cycle and the second messenger function of ceramide (1994) J. Biol. Chem., 269, pp. 3125-3128
  • Sweeley, C.C., Lipids and the modulation of cell function (1994) Curr. Top. Membr., 40, pp. 357-360
  • Castellani, O., Ribeiro, L.V., Fernandes, J.F., Differentiation of Trypanosoma cruzi in culture (1967) J. Protozool., 14, pp. 447-454
  • Bertello, L.E., Gonçalves, M.F., Colli, W., Lederkremer, R.M., Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms (1995) Biochem. J., 310, pp. 255-261
  • Ferguson, M.A.J., Glycosyl-phosphatidylinositol membrane anchors: The tale of a tail (1992) Biochem. Soc. Trans., 20, pp. 243-256
  • Uhrig, M.L., Couto, A.S., Colli, W., Lederkremer, R.M., Characterization of inositolphospholipids in Trypanosoma cruzi trypomastigote forms Biochim. Biophys. Acta.
  • Doering, T.L., Pessin, M.S., Hart, G.W., Raben, D.M., Englund, P.T., The fatty acids in unremodelled trypanosome glycosyl-phosphatidylinositols (1994) Biochem. J., 299, pp. 741-746
  • Olivera, A., Buckley, N.E., Spiegel, S., Sphingomyelinase and cell-permeable ceramide analogs stimulate cellular proliferation in Quiescent Swiss 3T3 fibroblasts (1992) J. Biol. Chem., 267, pp. 26121-26127

Citas:

---------- APA ----------
Bertello, L.E., Andrews, N.W. & De Lederkremer, R.M. (1996) . Developmentally regulated expression of ceramide in Trypanosoma cruzi. Molecular and Biochemical Parasitology, 79(2), 143-151.
http://dx.doi.org/10.1016/0166-6851(96)02645-X
---------- CHICAGO ----------
Bertello, L.E., Andrews, N.W., De Lederkremer, R.M. "Developmentally regulated expression of ceramide in Trypanosoma cruzi" . Molecular and Biochemical Parasitology 79, no. 2 (1996) : 143-151.
http://dx.doi.org/10.1016/0166-6851(96)02645-X
---------- MLA ----------
Bertello, L.E., Andrews, N.W., De Lederkremer, R.M. "Developmentally regulated expression of ceramide in Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 79, no. 2, 1996, pp. 143-151.
http://dx.doi.org/10.1016/0166-6851(96)02645-X
---------- VANCOUVER ----------
Bertello, L.E., Andrews, N.W., De Lederkremer, R.M. Developmentally regulated expression of ceramide in Trypanosoma cruzi. MOL. BIOCHEM. PARASITOL. 1996;79(2):143-151.
http://dx.doi.org/10.1016/0166-6851(96)02645-X