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Cruzi, T.; Ogueta, S.; Intosh, G.M.; Téllez-Iñon, Ma.T. "Regulation of Ca2+/calmodulin-dependent protein kinase from" (1996) Molecular and Biochemical Parasitology. 78(1-2):171-183
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Abstract:

A multifunctional Ca2+/calmodulin-dependent protein kinase (TcCaM K) was purified and characterized from the cytosol of Trypanosoma cruzi epimastigote forms. Like mammalian CaM KII, TcCaM K has a broad substrate specificity and a similar subunit composition. Western blot analysis revealed that this TcCaM K possesses two subunits of 50 and 60 kDa, which exhibited autophosphorylating activity. A panel of monoclonal and polyclonal antibodies raised against rat brain CaM KII could also recognize TcCaM K. However, experimental evidence suggests a different conformational arrangement of the TcCaM K subunits. Like its mammalian counterpart, two highly active autonomous, Ca2+-independent, states of TcCaM K can be isolated. These states, caused by high phosphate incorporation, differ only in their extent of Ca2+/CaM-dependence. About 15-20% of the autophosphorylated TcCaM K can be reverted using protein phosphatase 2A, and, consequently, its Ca2+-dependent activity is also partially restored. The situation is somewhat different when the enzyme is linked to the cytoskeleton, as we have previously shown. The membrane-bound form is present only in the native form. Activation increases its protein kinase activity from 5-to 14-fold. In this study, we provide evidence of another form of TcCaM K present in soluble fractions of the parasite that can be isolated in autonomous states. Our results suggest that autophosphorylation of membrane-bound TcCaM K may be responsible for kinase release in a Ca2+/CaM-independent state. These properties of TcCaM K may play an important role in regulating Ca2+-dependent processes in the parasite.

Registro:

Documento: Artículo
Título:Regulation of Ca2+/calmodulin-dependent protein kinase from
Autor:Cruzi, T.; Ogueta, S.; Intosh, G.M.; Téllez-Iñon, Ma.T.
Filiación:Inst. Invest. en Ing. Genet. Y Biol., Molec. and Fac. de Cie. Exac. y Nat., UBA, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
INGEBI, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Jules Stein Eye Institute, UCLA School of Medicine, 100 Stein Plaza, Los Angeles, CA 90024-7008, United States
Palabras clave:/cam-dependent protein kinase; Ca2+; Regulation; Trypanosoma cruzi; Mammalia; Trypanosoma; Trypanosoma cruzi; protein kinase (calcium,calmodulin); monoclonal antibody; peptide; protein kinase (calcium,calmodulin); protozoon antibody; syntide 2; syntide-2; article; autophosphorylation; calcium transport; enzyme purification; enzyme regulation; enzyme specificity; enzyme subunit; membrane binding; priority journal; trypanosoma cruzi; amino acid sequence; animal; biological model; chemistry; enzymology; immunology; isolation and purification; kinetics; metabolism; molecular genetics; molecular weight; phosphorylation; protein conformation; rat; solubility; Trypanosoma cruzi; Amino Acid Sequence; Animals; Antibodies, Monoclonal; Antibodies, Protozoan; Ca(2+)-Calmodulin Dependent Protein Kinase; Kinetics; Models, Biological; Molecular Sequence Data; Molecular Weight; Peptides; Phosphorylation; Protein Conformation; Rats; Solubility; Substrate Specificity; Trypanosoma cruzi
Año:1996
Volumen:78
Número:1-2
Página de inicio:171
Página de fin:183
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:protein kinase (calcium,calmodulin), 97350-82-8; Antibodies, Monoclonal; Antibodies, Protozoan; Ca(2+)-Calmodulin Dependent Protein Kinase, EC 2.7.1.123; Peptides; syntide-2, 108334-68-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v78_n1-2_p171_Cruzi

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Citas:

---------- APA ----------
Cruzi, T., Ogueta, S., Intosh, G.M. & Téllez-Iñon, Ma.T. (1996) . Regulation of Ca2+/calmodulin-dependent protein kinase from. Molecular and Biochemical Parasitology, 78(1-2), 171-183.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v78_n1-2_p171_Cruzi [ ]
---------- CHICAGO ----------
Cruzi, T., Ogueta, S., Intosh, G.M., Téllez-Iñon, Ma.T. "Regulation of Ca2+/calmodulin-dependent protein kinase from" . Molecular and Biochemical Parasitology 78, no. 1-2 (1996) : 171-183.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v78_n1-2_p171_Cruzi [ ]
---------- MLA ----------
Cruzi, T., Ogueta, S., Intosh, G.M., Téllez-Iñon, Ma.T. "Regulation of Ca2+/calmodulin-dependent protein kinase from" . Molecular and Biochemical Parasitology, vol. 78, no. 1-2, 1996, pp. 171-183.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v78_n1-2_p171_Cruzi [ ]
---------- VANCOUVER ----------
Cruzi, T., Ogueta, S., Intosh, G.M., Téllez-Iñon, Ma.T. Regulation of Ca2+/calmodulin-dependent protein kinase from. Mol. Biochem. Parasitol. 1996;78(1-2):171-183.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v78_n1-2_p171_Cruzi [ ]