Registro:
Documento: |
Artículo
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Título: | Purification and partial characterization of the cytosolic malate dehydrogenase from protoscolices of Echinococcus granulosus |
Autor: | Agüero, F.; Repetto, Y.; Hellman, U.; Cazzulo, J.J. |
Filiación: | Instituto de Investigaciones Bioquímicas Luis F. Leloir, Fundación Campomar - CONICET - Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, A. Machado 151, 1405 Buenos Aires, Argentina Departamento de Bioquímica, Facultad de Medicina (Sede Norte), Universidad de Chile, Santiago, Chile Ludwig Institute for Cancer Research, Uppsala Branch, Uppsala, Sweden
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Palabras clave: | cMDH; Echinococcus granulosus; Malate dehydrogenase; Protoscolices; malate dehydrogenase; article; controlled study; echinococcus granulosus; enzyme analysis; enzyme purification; nonhuman; priority journal; Amino Acid Sequence; Animal; Cytosol; Echinococcosis, Hepatic; Echinococcus; Helminth Proteins; Kinetics; Malate Dehydrogenase; Molecular Sequence Data; Sheep; Sheep Diseases; Substrate Specificity; Support, Non-U.S. Gov't; Echinococcus granulosus |
Año: | 1995
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Volumen: | 72
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Número: | 1-2
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Página de inicio: | 247
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Página de fin: | 251
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DOI: |
http://dx.doi.org/10.1016/0166-6851(95)00093-G |
Título revista: | Molecular and Biochemical Parasitology
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Título revista abreviado: | Mol. Biochem. Parasitol.
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ISSN: | 01666851
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CODEN: | MBIPD
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CAS: | Helminth Proteins; Malate Dehydrogenase, EC 1.1.1.37
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v72_n1-2_p247_Aguero |
Referencias:
- Agosin, Repetto, Studies on the metabolism of Echinococcus granulosus. VII. Reactions of the tricarboxylic acid cycle in Echinococcus granulosus scolices (1963) Comp. Biochem. Physiol., 8, pp. 245-261
- Agosin, Repetto, Studies on the metabolism of Echinococcus granulosus VIII The pathway to succinate in Echinococcus granulosus scolices (1963) Comparative Biochemistry and Physiology, 14, pp. 299-309
- Dicowsky, Repetto, Agosin, Studies on the metabolism of Echinococcus granulosus X The mechanism of production of volatile fatty acids (1963) Comparative Biochemistry and Physiology, 24, pp. 763-772
- McManus, Smyth, Intermediary carbohydrate metabolism in protoscoleces of Echinococcus granulosus (horse and sheep strains) and E. multilocularis (1982) Parasitology, 84, pp. 351-366
- Banaszak, Bradshaw, Malate dehydrogenases (1975) The Enzymes, 11, pp. 369-396. , P.D. Boyer, 3rd. Edn., Academic Press, New York and London
- Tielens, Energy generation in parasitic helminths (1994) Parasitol. Today, 10, pp. 346-352
- McManus, Bryant, Biochemistry and physiology of Echinococcus (1986) The Biology of Echinococcus and Hidatid Disease, pp. 114-142. , R.C.A. Thompson, George Allen and Unwin, London
- Rodrigues, Ferreira, Zaha, Molecular cloning and characterization of an Echinococcus granulosus cDNA encoding malate dehydrogenase (1993) Mol. Biochem. Parasitol., 60, pp. 157-160
- Andrews, The gel filtration behaviour of proteins related to their molecular weights over a wide range (1965) Biochem. J., 96, pp. 595-606
- Weber, Osborn, The reliability of molecular weight determination by dodecylsulfate-polyacrylamide gel electrophoresis (1969) J. Biol. Chem., 244, pp. 4406-4412
- Hellman, Wernstedt, Gonez, Helldin, Improvement of an “in gel” digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing (1995) Anal. Biochem., 224, pp. 451-455
- Dupourque, Kun, Cytoplasmic and mitochondrial malate dehydrogenase from beef kidney (1969) Methods Enzymol., 13, pp. 116-122
- Vessal, Bambaea-Row, Partial purification and comparison of the kinetic properties of ovine liver Echinococcus granulosus hydatid cyst fluid malate dehydrogenase and the cytoplasmic enzyme from the host liver (1994) Comp. Biochem. Physiol., 107, pp. 447-451
- Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-695
- Oakley, Kirsch, Morris, A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels (1980) Anal. Biochem., 105, pp. 361-363
- Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254
- Wilkinson, Statistical estimations in enzyme kinetics (1961) Biochem. J., 80, pp. 324-332
Citas:
---------- APA ----------
Agüero, F., Repetto, Y., Hellman, U. & Cazzulo, J.J.
(1995)
. Purification and partial characterization of the cytosolic malate dehydrogenase from protoscolices of Echinococcus granulosus. Molecular and Biochemical Parasitology, 72(1-2), 247-251.
http://dx.doi.org/10.1016/0166-6851(95)00093-G---------- CHICAGO ----------
Agüero, F., Repetto, Y., Hellman, U., Cazzulo, J.J.
"Purification and partial characterization of the cytosolic malate dehydrogenase from protoscolices of Echinococcus granulosus"
. Molecular and Biochemical Parasitology 72, no. 1-2
(1995) : 247-251.
http://dx.doi.org/10.1016/0166-6851(95)00093-G---------- MLA ----------
Agüero, F., Repetto, Y., Hellman, U., Cazzulo, J.J.
"Purification and partial characterization of the cytosolic malate dehydrogenase from protoscolices of Echinococcus granulosus"
. Molecular and Biochemical Parasitology, vol. 72, no. 1-2, 1995, pp. 247-251.
http://dx.doi.org/10.1016/0166-6851(95)00093-G---------- VANCOUVER ----------
Agüero, F., Repetto, Y., Hellman, U., Cazzulo, J.J. Purification and partial characterization of the cytosolic malate dehydrogenase from protoscolices of Echinococcus granulosus. Mol. Biochem. Parasitol. 1995;72(1-2):247-251.
http://dx.doi.org/10.1016/0166-6851(95)00093-G