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Abstract:

Four Trypanosoma cruzi genomic DNA fragments carrying different TcP2β genes have been isolated and sequenced. Three of them had a single TcP2β gene, while the 3.8-kb-long DNA segment encoding the TcP2β-H1.8 locus showed two TcP2β genes arranged in tandem. These genes were physically connected by a 428-bp-long DNA sequence that was also located immediately 5′ to the first gene and immediately 3′ to the second. Comparison of the 4 TcP2β gene loci, suggested that the insertion of this repeated element originated the duplication of its target sequence, a poly(dT) stretch. Approximately 1200 copies of this short sequence, named short interspersed repetitive element (SIRE), were found scattered in the genome. Analysis of the 5′ non-coding regions of different TcP2β mRNAs, and RNA-PCR experiments suggested that the insertion of a SIRE upstream of a TcP2β-H 1.8 gene introduced a new 3′ spliced leader (SL) acceptor site in the TcP2β-H1.8 pre-mRNAs, encoded within the SIRE. Consequently, in the mature H1.8 mRNA the SL sequence is followed by 38 bases directly transcribed from the SIRE. Structural and functional features of this repeated element reveal similarity to the short interspersed repetitive DNA sequences detected in the genomes of several microorganisms. © 1994.

Registro:

Documento: Artículo
Título:A short interspersed repetitive element provides a new 3′ acceptor site for trans-splicing in certain ribosomal P2β protein genes of Trypanosoma cruzi
Autor:Vazquez, M.P.; Schijman, A.G.; Levin, M.J.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biologia Molecular (INGEBI), Vuelta de Obligado 2490, 1428 Buenos, Aires, Argentina
Palabras clave:Repetitive sequence; Ribosomal protein; Spliced leader; Trans-splicing; Trypanosoma cruzi; repetitive dna; ribosome protein; animal experiment; article; controlled study; dna sequence; genetic transcription; nonhuman; priority journal; rna splicing; trypanosoma cruzi; Animal; Base Sequence; Binding Sites; DNA, Protozoan; Genes, Protozoan; Molecular Sequence Data; Multigene Family; Phosphoproteins; Repetitive Sequences, Nucleic Acid; Ribosomal Proteins; RNA Splicing; RNA, Messenger; RNA, Protozoan; Support, Non-U.S. Gov't; Trypanosoma cruzi; Animalia; Trypanosoma; Trypanosoma cruzi
Año:1994
Volumen:64
Número:2
Página de inicio:327
Página de fin:336
DOI: http://dx.doi.org/10.1016/0166-6851(94)00026-3
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:DNA, Protozoan; phosphoprotein P2; Phosphoproteins; Ribosomal Proteins; RNA, Messenger; RNA, Protozoan
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v64_n2_p327_Vazquez

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Citas:

---------- APA ----------
Vazquez, M.P., Schijman, A.G. & Levin, M.J. (1994) . A short interspersed repetitive element provides a new 3′ acceptor site for trans-splicing in certain ribosomal P2β protein genes of Trypanosoma cruzi. Molecular and Biochemical Parasitology, 64(2), 327-336.
http://dx.doi.org/10.1016/0166-6851(94)00026-3
---------- CHICAGO ----------
Vazquez, M.P., Schijman, A.G., Levin, M.J. "A short interspersed repetitive element provides a new 3′ acceptor site for trans-splicing in certain ribosomal P2β protein genes of Trypanosoma cruzi" . Molecular and Biochemical Parasitology 64, no. 2 (1994) : 327-336.
http://dx.doi.org/10.1016/0166-6851(94)00026-3
---------- MLA ----------
Vazquez, M.P., Schijman, A.G., Levin, M.J. "A short interspersed repetitive element provides a new 3′ acceptor site for trans-splicing in certain ribosomal P2β protein genes of Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 64, no. 2, 1994, pp. 327-336.
http://dx.doi.org/10.1016/0166-6851(94)00026-3
---------- VANCOUVER ----------
Vazquez, M.P., Schijman, A.G., Levin, M.J. A short interspersed repetitive element provides a new 3′ acceptor site for trans-splicing in certain ribosomal P2β protein genes of Trypanosoma cruzi. Mol. Biochem. Parasitol. 1994;64(2):327-336.
http://dx.doi.org/10.1016/0166-6851(94)00026-3