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Abstract:

We demonstrate that cruzipain, the major cysteine proteinase of Trypanosoma cruzi epimastigotes, is encoded by a large number of tandemly arranged genes. Restriction enzyme analysis of 20 clones containing complete repeat units of the gene, as well as sequencing of 2 of these clones, and comparison with previously published partial sequences, indicated that the sequence is conserved among the repeat units, although polymorphisms clearly exist. The repeat units contain an intergenic region of 528 bp and coding regions for pre- and pro-enzyme, a central domain and a C-terminal extension. The predicted amino acid sequences of these regions indicated a sequence identity of 30, 60, 70 and 36%, respectively, when the T. cruzi sequence was compared with the sequence of a similar cysteine proteinase from Trypanosoma brucei [14]. Studies by pulsed field gel electrophoresis, complemented with restriction analysis, indicated that the clusters are located on 2 4 different chromosomes in several parasite isolates. © 1992.

Registro:

Documento: Artículo
Título:The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes
Autor:Campetella, O.; Henriksson, J.; Åslund, U.; Frasch, A.C.C.; Pettersson, U.; Cazzulo, J.J.
Filiación:Instituto de Investigaciones Bioquímicas Fundación Campomar, CONICET, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Department of Medical Genetics, Biomedical Center, Uppsala University, Uppsala, Sweden
Palabras clave:Chromosomal localization; Cruzipain; Cysteine proteinase; Tandemly repeated genes; Trypanosoma cruzi; cysteine proteinase; amino acid sequence; animal cell; article; chromosome; epimastigote; genetic analysis; immunoblotting; nonhuman; priority journal; trypanosoma cruzi; Amino Acid Sequence; Animal; Base Sequence; Blotting, Northern; Blotting, Southern; Chromosome Mapping; Cloning, Molecular; Cysteine Endopeptidases; DNA; DNA Restriction Enzymes; Electrophoresis, Gel, Pulsed-Field; Molecular Sequence Data; Multigene Family; Polymorphism (Genetics); Repetitive Sequences, Nucleic Acid; RNA, Messenger; Support, Non-U.S. Gov't; Trypanosoma cruzi; Animalia; Trypanosoma; Trypanosoma brucei; Trypanosoma cruzi
Año:1992
Volumen:50
Número:2
Página de inicio:225
Página de fin:234
DOI: http://dx.doi.org/10.1016/0166-6851(92)90219-A
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:cruzipain, EC 3.4.22.-; Cysteine Endopeptidases, EC 3.4.22; DNA Restriction Enzymes, EC 3.1.21; DNA, 9007-49-2; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v50_n2_p225_Campetella

Referencias:

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  • Cazzulo, Hellman, Couso, Parodi, Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi (1990) Mol. Biochem. Parasitol., 38, pp. 41-48
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Citas:

---------- APA ----------
Campetella, O., Henriksson, J., Åslund, U., Frasch, A.C.C., Pettersson, U. & Cazzulo, J.J. (1992) . The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes. Molecular and Biochemical Parasitology, 50(2), 225-234.
http://dx.doi.org/10.1016/0166-6851(92)90219-A
---------- CHICAGO ----------
Campetella, O., Henriksson, J., Åslund, U., Frasch, A.C.C., Pettersson, U., Cazzulo, J.J. "The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes" . Molecular and Biochemical Parasitology 50, no. 2 (1992) : 225-234.
http://dx.doi.org/10.1016/0166-6851(92)90219-A
---------- MLA ----------
Campetella, O., Henriksson, J., Åslund, U., Frasch, A.C.C., Pettersson, U., Cazzulo, J.J. "The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes" . Molecular and Biochemical Parasitology, vol. 50, no. 2, 1992, pp. 225-234.
http://dx.doi.org/10.1016/0166-6851(92)90219-A
---------- VANCOUVER ----------
Campetella, O., Henriksson, J., Åslund, U., Frasch, A.C.C., Pettersson, U., Cazzulo, J.J. The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes. Mol. Biochem. Parasitol. 1992;50(2):225-234.
http://dx.doi.org/10.1016/0166-6851(92)90219-A