Abstract:
The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [ 14 C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding to the lectin was lost on treatment of Tc-85 with neuraminidase. The N-linked asialo-oligosaccharide was released by endo-β-N-acetylglucosaminidase F digestion of asialo-Tc-85 and was further analyzed using specific exoglycosidases. [ 14 C]fucose was detected after α-l-fucosidase treatment or mild acid hydrolysis. The afucosyl oligosaccharide was 3 H-labeled by the galactose oxidase-NaB 3 H 4 method. [ 3 H]Galactose was released by α-galactosidase, and only then was β-galactosidase effective in removing another galactose. The gal(α1-3)gal unit was demonstrated by periodate oxidation studies on the [ 3 H]galactose-labeled asialo-glycoprotein. The presence of gal(α1-3)gal in Tc-85 could be related to the recent finding of elevated antibody levels against this epitope in patients with Chagas' disease. © 1990.
Registro:
Documento: |
Artículo
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Título: | The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units |
Autor: | Couto, A.S.; Goncalves, M.F.; Colli, W.; De Lederkremer, R.M. |
Filiación: | Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
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Palabras clave: | Gal(α1-3)gal; Glycoprotein; Tc-85; Trypanosoma cruzi; epitope; membrane antigen; animal cell; article; chromatography; glycoprotein structure; nonhuman; priority journal; protozoon; trypanosoma cruzi; trypomastigote; Acetylglucosaminidase; Animal; Disaccharides; Fucose; Glycoproteins; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; Molecular Structure; Protozoan Proteins; Sialic Acids; Support, Non-U.S. Gov't; Trypanosoma cruzi; Animalia; Protozoa; Triticum aestivum; Trypanosoma; Trypanosoma cruzi |
Año: | 1990
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Volumen: | 39
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Número: | 1
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Página de inicio: | 101
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Página de fin: | 107
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DOI: |
http://dx.doi.org/10.1016/0166-6851(90)90012-B |
Título revista: | Molecular and Biochemical Parasitology
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Título revista abreviado: | Mol. Biochem. Parasitol.
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ISSN: | 01666851
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CODEN: | MBIPD
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CAS: | Acetylglucosaminidase, EC 3.2.1.30; Disaccharides; Fucose, 3713-31-3; galactosyl-(1-3)galactose, 13168-24-6; Glycoproteins; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, EC 3.2.1.96; Protozoan Proteins; Sialic Acids
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v39_n1_p101_Couto |
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Citas:
---------- APA ----------
Couto, A.S., Goncalves, M.F., Colli, W. & De Lederkremer, R.M.
(1990)
. The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units. Molecular and Biochemical Parasitology, 39(1), 101-107.
http://dx.doi.org/10.1016/0166-6851(90)90012-B---------- CHICAGO ----------
Couto, A.S., Goncalves, M.F., Colli, W., De Lederkremer, R.M.
"The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units"
. Molecular and Biochemical Parasitology 39, no. 1
(1990) : 101-107.
http://dx.doi.org/10.1016/0166-6851(90)90012-B---------- MLA ----------
Couto, A.S., Goncalves, M.F., Colli, W., De Lederkremer, R.M.
"The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units"
. Molecular and Biochemical Parasitology, vol. 39, no. 1, 1990, pp. 101-107.
http://dx.doi.org/10.1016/0166-6851(90)90012-B---------- VANCOUVER ----------
Couto, A.S., Goncalves, M.F., Colli, W., De Lederkremer, R.M. The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units. Mol. Biochem. Parasitol. 1990;39(1):101-107.
http://dx.doi.org/10.1016/0166-6851(90)90012-B