Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [ 14 C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding to the lectin was lost on treatment of Tc-85 with neuraminidase. The N-linked asialo-oligosaccharide was released by endo-β-N-acetylglucosaminidase F digestion of asialo-Tc-85 and was further analyzed using specific exoglycosidases. [ 14 C]fucose was detected after α-l-fucosidase treatment or mild acid hydrolysis. The afucosyl oligosaccharide was 3 H-labeled by the galactose oxidase-NaB 3 H 4 method. [ 3 H]Galactose was released by α-galactosidase, and only then was β-galactosidase effective in removing another galactose. The gal(α1-3)gal unit was demonstrated by periodate oxidation studies on the [ 3 H]galactose-labeled asialo-glycoprotein. The presence of gal(α1-3)gal in Tc-85 could be related to the recent finding of elevated antibody levels against this epitope in patients with Chagas' disease. © 1990.

Registro:

Documento: Artículo
Título:The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
Autor:Couto, A.S.; Goncalves, M.F.; Colli, W.; De Lederkremer, R.M.
Filiación:Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
Palabras clave:Gal(α1-3)gal; Glycoprotein; Tc-85; Trypanosoma cruzi; epitope; membrane antigen; animal cell; article; chromatography; glycoprotein structure; nonhuman; priority journal; protozoon; trypanosoma cruzi; trypomastigote; Acetylglucosaminidase; Animal; Disaccharides; Fucose; Glycoproteins; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; Molecular Structure; Protozoan Proteins; Sialic Acids; Support, Non-U.S. Gov't; Trypanosoma cruzi; Animalia; Protozoa; Triticum aestivum; Trypanosoma; Trypanosoma cruzi
Año:1990
Volumen:39
Número:1
Página de inicio:101
Página de fin:107
DOI: http://dx.doi.org/10.1016/0166-6851(90)90012-B
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:Acetylglucosaminidase, EC 3.2.1.30; Disaccharides; Fucose, 3713-31-3; galactosyl-(1-3)galactose, 13168-24-6; Glycoproteins; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, EC 3.2.1.96; Protozoan Proteins; Sialic Acids
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v39_n1_p101_Couto

Referencias:

  • Handman, Mc Conville, Goding, Carbohydrate antigens as possible parasite vaccines (1987) Immunol. Today, 8, pp. 181-185
  • Zingales, Katzin, Arruda, Colli, Correlation of tunicamycin sensitive surface glycoproteins from Trypanosoma cruzi with parasite interiorization into mammalian cells (1985) Mol. Biochem. Parasitol., 16, pp. 21-34
  • Alves, Abuin, Kuwajima, Colli, Partial inhibition of trypomastigote entry into cultured mammalian cells by monoclonal antibodies against a surface glycoprotein of Trypanosoma cruzi (1986) Mol. Biochem. Parasitol., 21, pp. 75-82
  • Katzin, Colli, Lectin receptors in Trypanosoma cruzi: an N-acetyl-d-glucosamine containing surface glycoprotein specific for the trypomastigote stage (1983) Biochim. Biophys. Acta, 727, pp. 403-411
  • Peterson, Wrightsman, Manning, Cloning of a major surface-antigen gene of Trypanosoma cruzi and identification of a nonapeptide repeat (1986) Nature, 322, pp. 566-568
  • Ibañez, Affranchino, Frasch, Antigenic determinants of Trypanosoma cruzi defined by cloning of parasite DNA (1987) Mol. Biochem. Parasitol., 25, pp. 175-184
  • Ibañez, Affranchino, Macina, Reyes, Leguizamon, Camargo, Aslund, Frasch, Multiple Trypanosoma cruzi antigens containing tandemly repeated aminoacid sequence motifs (1988) Mol. Biochem. Parasitol., 30, pp. 27-34
  • Couto, Katzin, Colli, Lederkremer, Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi (1987) Mol. Biochem. Parasitol., 26, pp. 145-154
  • Andrews, Katzin, Colli, Mapping of surface glycoproteins of Trypanosoma cruzi by two-dimensional electrophoresis (1984) A correlation with the cell invasion capacity, 140, pp. 599-604. , 2nd ed., Eur. J. Biochem
  • Hodges, Laine, Chan, Structure of the oligosaccharide chains in human α 1 -protease inhibitor (1979) J. Biol. Chem., 254, pp. 8208-8212
  • Ogata, Lloyd, Mild alkaline borohydride treatment of glycoproteins (1982) A method for liberating both N- and O-linked carbohydrate chains, 119, pp. 351-359. , 2nd ed., Anal. Biochem
  • Bhavanandan, Katlic, The interaction of wheat germ agglutinin with sialoglycoproteins (1979) The role of sialic acid, 254, pp. 4000-4008. , 2nd ed., J. Biol. Chem
  • Doyle, Canal, Engel, Parodi, Characterization of the mechanism of protein glycosylation and the structure of glycoconjugates in tissue culture trypomastigotes and intracellular amastigotes of Trypanosoma cruzi (1986) Mol. Biochem. Parasitol., 21, pp. 93-101
  • Maradufu, Perlin, A non-hydrogen bonding role for the 4-hydroxyl group of d-galactose in its reaction with d-galactose oxidase (1974) Carbohydr. Res., 32, pp. 93-99
  • Avila, Rojas, Towbin, Serological activity against galactosyl-α(1–3)galactose in sera from patients with several kinetoplastida infections (1988) J. Clin. Microbiol., 26, pp. 126-132
  • Pereira, A developmentally regulated neuraminidase activity in Trypanosoma cruzi (1983) Science, 219, pp. 1444-1446
  • Pereira, Hoff, Heterogeneous distribution of neuraminidase activity in strains and clones of Trypanosoma cruzi and its possible association with parasite myotropism (1986) Mol. Biochem. Parasitol., 20, pp. 183-189
  • Harth, Haidaris, So, Neuraminidase from Trypanosoma cruzi: analysis of enhanced expression of the enzyme in infectious forms (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 8320-8324. , 2nd ed
  • Zingales, Carniol, Lederkremer, Colli, Direct sialic acid transfer from a protein donor to glycolipids of trypomastigote forms of Trypanosoma cruzi (1987) Mol. Biochem. Parasitol., 26, pp. 135-144

Citas:

---------- APA ----------
Couto, A.S., Goncalves, M.F., Colli, W. & De Lederkremer, R.M. (1990) . The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units. Molecular and Biochemical Parasitology, 39(1), 101-107.
http://dx.doi.org/10.1016/0166-6851(90)90012-B
---------- CHICAGO ----------
Couto, A.S., Goncalves, M.F., Colli, W., De Lederkremer, R.M. "The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units" . Molecular and Biochemical Parasitology 39, no. 1 (1990) : 101-107.
http://dx.doi.org/10.1016/0166-6851(90)90012-B
---------- MLA ----------
Couto, A.S., Goncalves, M.F., Colli, W., De Lederkremer, R.M. "The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units" . Molecular and Biochemical Parasitology, vol. 39, no. 1, 1990, pp. 101-107.
http://dx.doi.org/10.1016/0166-6851(90)90012-B
---------- VANCOUVER ----------
Couto, A.S., Goncalves, M.F., Colli, W., De Lederkremer, R.M. The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units. Mol. Biochem. Parasitol. 1990;39(1):101-107.
http://dx.doi.org/10.1016/0166-6851(90)90012-B