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Abstract:

The size of a lysosomal cysteine proteinase from epimastigotes of Trypanosoma cruzi decreased from 60 to 54 kDa upon treatment with endo β N acetylglucosaminidase H. A lower-molecular weight component (30-35 kDa), which usually accompanies the 60-kDa protein also increased its electrophoretic mobility, and seems to consist of a mixture of degradation products of the enzyme, since both the larger and the smaller components had the same N-terminal sequence as the 60-kDa protein. The amino acid composition of the protein moiety and the composition of the oligosaccharide chains have been determined. The oligosaccharide chains are of the high-mannose type, and contain 6, 7, 8 or 9 mannose residues, as shown both by in vivo labelling with [U-14C]glucose, and by labeling the endo β N acetylglucosaminidase H-sensitive oligosaccharides of the purified enzyme with tritiated sodium borohydride. The oligosaccharide chains did not contain phosphate residues. Further studies with [U-14C]-labeled total glycoproteins of T. cruzi, and enzyme assays, suggest that T. cruzi and other trypanosomatids do not target their lysosomal enzymes to the organelle through the mannose-6-phosphate marker pathway. © 1990.

Registro:

Documento: Artículo
Título:Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues
Autor:Cazzulo, J.J.; Hellman, U.; Couso, R.; Parodi, A.J.A.
Filiación:Instituto de Investigaciones Bioquímicas Fundacíon Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires - CONICET, Buenos Aires, Argentina
Ludwig Institute for Cancer Research, Uppsala Branch, Uppsala, Sweden
Palabras clave:Amino acid composition; Cysteine proteinase; Lysosomal enzyme; Oligosaccharide composition; Trypanosoma cruzi; amino acid; carbohydrate; cysteine proteinase; mannose; article; lysosome; nonhuman; priority journal; protozoon; trypanosoma cruzi; Amino Acid Sequence; Amino Acids; Animal; Carbohydrate Sequence; Carbohydrates; Cysteine Endopeptidases; Lysosomes; Mannosephosphates; Molecular Sequence Data; Molecular Weight; Support, Non-U.S. Gov't; Trypanosoma cruzi; Protozoa; Trypanosoma; Trypanosoma cruzi
Año:1990
Volumen:38
Número:1
Página de inicio:41
Página de fin:48
DOI: http://dx.doi.org/10.1016/0166-6851(90)90203-X
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:Amino Acids; Carbohydrates; Cysteine Endopeptidases, EC 3.4.22; Mannosephosphates
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v38_n1_p41_Cazzulo

Referencias:

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Citas:

---------- APA ----------
Cazzulo, J.J., Hellman, U., Couso, R. & Parodi, A.J.A. (1990) . Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues. Molecular and Biochemical Parasitology, 38(1), 41-48.
http://dx.doi.org/10.1016/0166-6851(90)90203-X
---------- CHICAGO ----------
Cazzulo, J.J., Hellman, U., Couso, R., Parodi, A.J.A. "Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues" . Molecular and Biochemical Parasitology 38, no. 1 (1990) : 41-48.
http://dx.doi.org/10.1016/0166-6851(90)90203-X
---------- MLA ----------
Cazzulo, J.J., Hellman, U., Couso, R., Parodi, A.J.A. "Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues" . Molecular and Biochemical Parasitology, vol. 38, no. 1, 1990, pp. 41-48.
http://dx.doi.org/10.1016/0166-6851(90)90203-X
---------- VANCOUVER ----------
Cazzulo, J.J., Hellman, U., Couso, R., Parodi, A.J.A. Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues. Mol. Biochem. Parasitol. 1990;38(1):41-48.
http://dx.doi.org/10.1016/0166-6851(90)90203-X