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Abstract:

A protein kinase C activity from epimastigote forms of Trypanosoma cruzi was characterized. Cytosolic extracts were chromatographed on DEAE-cellulose columns giving two peaks of kinase activity which were eluted at 0.1 and 0.15 M NaCl. The first activity peak requires Ca2+ and phosphatidylserine for activity. Further kinase purification was performed by chromatography on phenyl Sepharose columns. In these columns the enzyme activity was adsorbed in the presence of Ca2+ and eluted with a EGTA-containing buffer. T. cruzi protein kinase C activity preferentially phosphorylated histone H1. It was stimulated by diacylglycerol and phorbol myristate acetate, and inhibited by polymyxin B and staurosporine. After subcellular fractionation of epimastigote cells, the kinase was found to be associated with microsomal and cytosolic fractions. © 1989.

Registro:

Documento: Artículo
Título:Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization
Autor:Gomez, M.L.; Erijman, L.; Arauzo, S.; Torres, H.N.; Tellez-Inon, M.T.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Buenos Aires, Argentina
Palabras clave:Diacylglycerol; Phorbol ester; Protein kinase C; Staurosporine; Trypanosoma cruzi; protein kinase c; animal cell; enzyme activity; epimastigote; nonhuman; protozoon; trypanosoma cruzi; Animal; Calcium; Chromatography, Agarose; Chromatography, DEAE-Cellulose; Cytosol; Diglycerides; Egtazic Acid; Histones; Microsomes; Phosphatidylserines; Phosphorylation; Protein Kinase C; Support, Non-U.S. Gov't; Tetradecanoylphorbol Acetate; Trypanosoma cruzi
Año:1989
Volumen:36
Número:2
Página de inicio:101
Página de fin:108
DOI: http://dx.doi.org/10.1016/0166-6851(89)90182-5
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:protein kinase C, 141436-78-4; Calcium, 7440-70-2; Diglycerides; Egtazic Acid, 67-42-5; Histones; Phosphatidylserines; Protein Kinase C, EC 2.7.1.37; Tetradecanoylphorbol Acetate, 16561-29-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v36_n2_p101_Gomez

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Citas:

---------- APA ----------
Gomez, M.L., Erijman, L., Arauzo, S., Torres, H.N. & Tellez-Inon, M.T. (1989) . Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization. Molecular and Biochemical Parasitology, 36(2), 101-108.
http://dx.doi.org/10.1016/0166-6851(89)90182-5
---------- CHICAGO ----------
Gomez, M.L., Erijman, L., Arauzo, S., Torres, H.N., Tellez-Inon, M.T. "Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization" . Molecular and Biochemical Parasitology 36, no. 2 (1989) : 101-108.
http://dx.doi.org/10.1016/0166-6851(89)90182-5
---------- MLA ----------
Gomez, M.L., Erijman, L., Arauzo, S., Torres, H.N., Tellez-Inon, M.T. "Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization" . Molecular and Biochemical Parasitology, vol. 36, no. 2, 1989, pp. 101-108.
http://dx.doi.org/10.1016/0166-6851(89)90182-5
---------- VANCOUVER ----------
Gomez, M.L., Erijman, L., Arauzo, S., Torres, H.N., Tellez-Inon, M.T. Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization. Mol. Biochem. Parasitol. 1989;36(2):101-108.
http://dx.doi.org/10.1016/0166-6851(89)90182-5