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Abstract:

The carbohydrate moiety of the Tc-85 surface glycoprotein from the infective trypomastigote form of Trypanosoma cruzi was analysed. Tc-85 could be metabolically labeled by incubation of the cells with d-[14C]mannose or d-[14C]glucose. Degradation techniques were performed directly on the polyacrylamide gel band containing labeled Tc-85. A mannobiose was cleaved by β-elimination and further treatment of the remaining material under conditions which liberate N-asparaginyl linkages, released a complex oligosaccharide. The presence of sialic acid was demonstrated by: mild acid hydrolysis, neuraminidase treatment and periodate oxidation under mild conditions followed by NaB3H4 reduction, hydrolysis, and detection of NANA7 by paper electrophoresis. In addition, the chromatographic behavior of the asialooligosaccharide was significantly different from that of the original sample. Galactose, mannose and glucosamine are the other monosaccharide components of the sialooligosaccharide. © 1987.

Registro:

Documento: Artículo
Título:Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi
Autor:Couto, A.S.; Katzin, A.M.; Colli, W.; de Lederkremer, R.M.
Filiación:Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Glycoprotein carbohydrate composition; Sialic acid; Trypanosoma cruzi; Trypomastigote; glycoprotein; membrane protein; n acetylneuraminic acid; oligosaccharide; sialic acid derivative; affinity chromatography; animal; article; gel chromatography; paper chromatography; paper electrophoresis; Trypanosoma cruzi; Animal; Chromatography, Affinity; Chromatography, Gel; Chromatography, Paper; Electrophoresis, Paper; Glycoproteins; Membrane Proteins; N-Acetylneuraminic Acid; Oligosaccharides; Sialic Acids; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1987
Volumen:26
Número:1-2
Página de inicio:145
Página de fin:153
DOI: http://dx.doi.org/10.1016/0166-6851(87)90138-1
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:n acetylneuraminic acid, 131-48-6; Glycoproteins; Membrane Proteins; N-Acetylneuraminic Acid, 131-48-6; Oligosaccharides; Sialic Acids
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v26_n1-2_p145_Couto

Referencias:

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  • Lederkremer, Casal, Couto, Colli, Structural studies on the oligosaccharide moiety of the lipopeptidophosphoglycan from Trypanosoma cruzi (1985) Eur. J. Biochem., 151, pp. 539-542
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  • Katzin, Colli, Lectin receptors in Trypanosoma cruzi: an N-acetyl-d-glucosamine containing surface glycoprotein specific for the trypomastigote stage (1983) Biochim. Biophys. Acta, 727, pp. 403-411
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Citas:

---------- APA ----------
Couto, A.S., Katzin, A.M., Colli, W. & de Lederkremer, R.M. (1987) . Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi. Molecular and Biochemical Parasitology, 26(1-2), 145-153.
http://dx.doi.org/10.1016/0166-6851(87)90138-1
---------- CHICAGO ----------
Couto, A.S., Katzin, A.M., Colli, W., de Lederkremer, R.M. "Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi" . Molecular and Biochemical Parasitology 26, no. 1-2 (1987) : 145-153.
http://dx.doi.org/10.1016/0166-6851(87)90138-1
---------- MLA ----------
Couto, A.S., Katzin, A.M., Colli, W., de Lederkremer, R.M. "Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 26, no. 1-2, 1987, pp. 145-153.
http://dx.doi.org/10.1016/0166-6851(87)90138-1
---------- VANCOUVER ----------
Couto, A.S., Katzin, A.M., Colli, W., de Lederkremer, R.M. Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi. Mol. Biochem. Parasitol. 1987;26(1-2):145-153.
http://dx.doi.org/10.1016/0166-6851(87)90138-1