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Abstract:

Labeled sialoglycolipids were purified from tissue culture-derived trypomastigotes incubated with [3H]fetuin. Thin layer chromatography of [3H]sialoglycolipids showed three components with the same migration as gangliosides extracted from parasites incubated with [3H]palmitic acid. Neuraminidase treatment or mild acid hydrolysis confirmed the presence of [3H]sialyl residues in sialoglycolipids synthesized after [3H]fetuin incubation. Labeling was not observed when parasites were incubated with free [3H]sialic acid (C7 derivative), suggesting that sialyl residues are directly transferred in vivo to gangliosides, by an enzymatic reaction possibly catalysed by a sialyl transferase (transglycosylase). Sonicated extracts of trypomastigotes incubated with [3H]fetuin catalysed the labeling of endogenous glycoconjugates as well as of bovine brain gangliosides. The transglycosylase activity was found associated with the particulate fraction and could be solubilized with Triton X-100. The specific activity of the sialic acid transglycosylase in epimastigotes is 17% of that found in trypomastigotes. Addition of an excess free sialic acid did not inhibit the reaction, suggesting that transfer does not occur via a pool of free sialic acid. © 1987.

Registro:

Documento: Artículo
Título:Direct sialic acid transfer from a protein donor to glycolipids of trypomastigote forms of Trypanosoma cruzi
Autor:Zingales, B.; Carniol, C.; de Lederkremer, R.M.; Colli, W.
Filiación:Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Sialic acid; Sialic acid transglycosylase; Sialidase; Sialoglycolipid; Sialyl transferase; Trypanosoma cruzi; alpha fetoprotein; beta D galactoside alpha 2 6 sialyltransferase; beta-D-galactoside alpha 2-6-sialyltransferase; glycoconjugate; glycolipid; sialic acid derivative; sialoglycolipids; sialyltransferase; animal; article; enzymology; metabolism; paper chromatography; thin layer chromatography; Trypanosoma cruzi; alpha-Fetoproteins; Animal; Chromatography, Paper; Chromatography, Thin Layer; Glycoconjugates; Glycolipids; Sialic Acids; Sialyltransferases; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1987
Volumen:26
Número:1-2
Página de inicio:135
Página de fin:144
DOI: http://dx.doi.org/10.1016/0166-6851(87)90137-X
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:sialyltransferase, 9075-81-4; alpha-Fetoproteins; beta-D-galactoside alpha 2-6-sialyltransferase, EC 2.4.99.1; Glycoconjugates; Glycolipids; Sialic Acids; sialoglycolipids; Sialyltransferases, EC 2.4.99.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v26_n1-2_p135_Zingales

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Citas:

---------- APA ----------
Zingales, B., Carniol, C., de Lederkremer, R.M. & Colli, W. (1987) . Direct sialic acid transfer from a protein donor to glycolipids of trypomastigote forms of Trypanosoma cruzi. Molecular and Biochemical Parasitology, 26(1-2), 135-144.
http://dx.doi.org/10.1016/0166-6851(87)90137-X
---------- CHICAGO ----------
Zingales, B., Carniol, C., de Lederkremer, R.M., Colli, W. "Direct sialic acid transfer from a protein donor to glycolipids of trypomastigote forms of Trypanosoma cruzi" . Molecular and Biochemical Parasitology 26, no. 1-2 (1987) : 135-144.
http://dx.doi.org/10.1016/0166-6851(87)90137-X
---------- MLA ----------
Zingales, B., Carniol, C., de Lederkremer, R.M., Colli, W. "Direct sialic acid transfer from a protein donor to glycolipids of trypomastigote forms of Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 26, no. 1-2, 1987, pp. 135-144.
http://dx.doi.org/10.1016/0166-6851(87)90137-X
---------- VANCOUVER ----------
Zingales, B., Carniol, C., de Lederkremer, R.M., Colli, W. Direct sialic acid transfer from a protein donor to glycolipids of trypomastigote forms of Trypanosoma cruzi. Mol. Biochem. Parasitol. 1987;26(1-2):135-144.
http://dx.doi.org/10.1016/0166-6851(87)90137-X