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Schoijet, A.C.; Miranda, K.; Sternlieb, T.; Barrera, N.M.; Girard-Dias, W.; de Souza, W.; Alonso, G.D. "TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei" (2018) Molecular and Biochemical Parasitology. 219:33-41
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Abstract:

The class III phosphatidylinositol 3-kinase (PI3K) Vps34 is an important regulator of key cellular functions, including cell growth, survival, intracellular trafficking, autophagy and nutrient sensing. In yeast, Vps34 is associated with the putative serine/threonine protein kinase Vps15, however, its role in signaling has not been deeply evaluated. Here, we have identified the Vps15 orthologue in Trypanosoma brucei, named TbVps15. Knockdown of TbVps15 expression by interference RNA resulted in inhibition of cell growth and blockage of cytokinesis. Scanning electron microcopy revealed a variety of morphological abnormalities, with enlarged parasites and dividing cells that often exhibited a detached flagellum. Transmission electron microscopy analysis of TbVps15 RNAi cells showed an increase in intracellular vacuoles of the endomembrane system and some cells displayed an enlargement of the flagellar pocket, a common feature of cells defective in endocytosis. Moreover, uptake of dextran, transferrin and Concanavalin A was impaired. Finally, TbVps15 downregulation affected the PI3K activity, supporting the hypothesis that TbVps15 and TbVps34 form a complex as occurs in other organisms. In summary, we propose that TbVps15 has a role in the maintenance of cytokinesis, endocytosis and intracellular trafficking in T. brucei. © 2017 Elsevier B.V.

Registro:

Documento: Artículo
Título:TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei
Autor:Schoijet, A.C.; Miranda, K.; Sternlieb, T.; Barrera, N.M.; Girard-Dias, W.; de Souza, W.; Alonso, G.D.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres” (INGEBI-CONICET), Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular, Buenos Aires, Argentina
Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho and Centro Nacional de Biologia Estrutural e Bioimagem (CENABIO), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
Instituto Nacional de Biologia Estrutural e Bioimagem, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
Plataforma de Microscopia Eletrônica Rudolf Barth IOC, Fiocruz, Rio de Janeiro, Brazil
Palabras clave:Cell cycle; Endocytosis; TbVps15 protein kinase; Trypanosoma brucei; concanavalin A; dextran; phosphatidylinositol 3 kinase; transferrin; phosphatidylinositol 3 kinase; protein binding; protein serine threonine kinase VPS15; animal cell; Article; cell growth; cell structure; cell vacuole; controlled study; cytokinesis; down regulation; endocytosis; enzyme activity; flagellum; growth inhibition; nonhuman; priority journal; protein expression; protein function; RNA interference; scanning electron microscopy; transmission electron microscopy; Trypanosoma brucei; atomic force microscopy; cytology; disease transmission; endocytosis; enzymology; gene knockdown; genetics; metabolism; physiology; Trypanosoma brucei brucei; Class III Phosphatidylinositol 3-Kinases; Cytokinesis; Disease Transmission, Infectious; Endocytosis; Gene Knockdown Techniques; Microscopy, Atomic Force; Microscopy, Electron, Transmission; Phosphatidylinositol 3-Kinase; Protein Binding; Trypanosoma brucei brucei; Vacuolar Sorting Protein VPS15
Año:2018
Volumen:219
Página de inicio:33
Página de fin:41
DOI: http://dx.doi.org/10.1016/j.molbiopara.2017.11.004
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:concanavalin A, 11028-71-0; dextran, 87915-38-6, 9014-78-2; phosphatidylinositol 3 kinase, 115926-52-8; transferrin, 82030-93-1; Class III Phosphatidylinositol 3-Kinases; Phosphatidylinositol 3-Kinase; Vacuolar Sorting Protein VPS15
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v219_n_p33_Schoijet

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Citas:

---------- APA ----------
Schoijet, A.C., Miranda, K., Sternlieb, T., Barrera, N.M., Girard-Dias, W., de Souza, W. & Alonso, G.D. (2018) . TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei. Molecular and Biochemical Parasitology, 219, 33-41.
http://dx.doi.org/10.1016/j.molbiopara.2017.11.004
---------- CHICAGO ----------
Schoijet, A.C., Miranda, K., Sternlieb, T., Barrera, N.M., Girard-Dias, W., de Souza, W., et al. "TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei" . Molecular and Biochemical Parasitology 219 (2018) : 33-41.
http://dx.doi.org/10.1016/j.molbiopara.2017.11.004
---------- MLA ----------
Schoijet, A.C., Miranda, K., Sternlieb, T., Barrera, N.M., Girard-Dias, W., de Souza, W., et al. "TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei" . Molecular and Biochemical Parasitology, vol. 219, 2018, pp. 33-41.
http://dx.doi.org/10.1016/j.molbiopara.2017.11.004
---------- VANCOUVER ----------
Schoijet, A.C., Miranda, K., Sternlieb, T., Barrera, N.M., Girard-Dias, W., de Souza, W., et al. TbVps15 is required for vesicular transport and cytokinesis in Trypanosoma brucei. Mol. Biochem. Parasitol. 2018;219:33-41.
http://dx.doi.org/10.1016/j.molbiopara.2017.11.004