Artículo

Musikant, D.; Ferri, G.; Durante, I.M.; Buscaglia, C.A.; Altschuler, D.L.; Edreira, M.M. "Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi" (2017) Molecular and Biochemical Parasitology. 211:67-70
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Abstract:

Mechanistic details of the modulation by cAMP of Trypanosoma cruzi host cell invasion remain ill-defined. Here we report that activation of host's Epac1 stimulated invasion, whereas specific pharmacological inhibition or maneuvers that alter Epac1 subcellular localization significantly reduced invasion. Furthermore, while specific activation of host cell PKA showed no effect, its inhibition resulted in an increased invasion, revealing a crosstalk between the PKA and Epac signaling pathways during the process of invasion. Therefore, our data suggests that subcellular localization of Epac might be playing an important role during invasion and that specific activation of the host cell cAMP/Epac1 pathway is required for cAMP-mediated invasion. © 2016 Elsevier B.V.

Registro:

Documento: Artículo
Título:Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi
Autor:Musikant, D.; Ferri, G.; Durante, I.M.; Buscaglia, C.A.; Altschuler, D.L.; Edreira, M.M.
Filiación:IQUIBICEN-CONICET-Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, UNSAM-CONICET, Buenos Aires, Argentina
Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, United States
Palabras clave:cAMP; Epac; Invasion; PKA; Trypanosoma cruzi; cyclic AMP; cyclic AMP dependent protein kinase; Epac1 protein; protozoal protein; unclassified drug; cyclic AMP; Epac-1 protein, rat; guanine nucleotide exchange factor; Article; cellular distribution; host cell; nonhuman; priority journal; protein localization; protein synthesis; signal transduction; Trypanosoma cruzi; animal; cell culture; host parasite interaction; metabolism; pathogenicity; physiology; protein transport; rat; Trypanosoma cruzi; Animals; Cells, Cultured; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Guanine Nucleotide Exchange Factors; Host-Parasite Interactions; Protein Transport; Rats; Signal Transduction; Trypanosoma cruzi
Año:2017
Volumen:211
Página de inicio:67
Página de fin:70
DOI: http://dx.doi.org/10.1016/j.molbiopara.2016.10.003
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:cyclic AMP, 60-92-4; cyclic AMP dependent protein kinase; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Epac-1 protein, rat; Guanine Nucleotide Exchange Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v211_n_p67_Musikant

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Citas:

---------- APA ----------
Musikant, D., Ferri, G., Durante, I.M., Buscaglia, C.A., Altschuler, D.L. & Edreira, M.M. (2017) . Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi. Molecular and Biochemical Parasitology, 211, 67-70.
http://dx.doi.org/10.1016/j.molbiopara.2016.10.003
---------- CHICAGO ----------
Musikant, D., Ferri, G., Durante, I.M., Buscaglia, C.A., Altschuler, D.L., Edreira, M.M. "Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi" . Molecular and Biochemical Parasitology 211 (2017) : 67-70.
http://dx.doi.org/10.1016/j.molbiopara.2016.10.003
---------- MLA ----------
Musikant, D., Ferri, G., Durante, I.M., Buscaglia, C.A., Altschuler, D.L., Edreira, M.M. "Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 211, 2017, pp. 67-70.
http://dx.doi.org/10.1016/j.molbiopara.2016.10.003
---------- VANCOUVER ----------
Musikant, D., Ferri, G., Durante, I.M., Buscaglia, C.A., Altschuler, D.L., Edreira, M.M. Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi. Mol. Biochem. Parasitol. 2017;211:67-70.
http://dx.doi.org/10.1016/j.molbiopara.2016.10.003