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Jäger, A.V.; De Gaudenzi, J.G.; Mild, J.G.; Cormack, B.M.; Pantano, S.; Altschuler, D.L.; Edreira, M.M. "Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi" (2014) Molecular and Biochemical Parasitology. 198(2):104-112
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Abstract:

Cyclic AMP has been implicated as second messenger in a wide range of cellular processes. In the protozoan parasite Trypanosoma cruzi, cAMP is involved in the development of the parasite's life cycle. While cAMP effectors have been widely studied in other eukaryotic cells, little is known about cAMP's mechanism of action in T. cruzi. To date, only a cAMP-dependent protein kinase A (PKA) has been cloned and characterised in this parasite; however experimental evidence indicates the existence of cAMP-dependent, PKA-independent events. In order to identify new cAMP binding proteins as potential cAMP effectors, we carried out in silico studies using the predicted T. cruzi proteome. Using a combination of search methods 27 proteins with putative cNMP binding domains (CBDs) were identified. Phylogenetic analysis of the CBDs presented a homogeneous distribution, with sequences segregated into two main branches: one containing kinases-like proteins and the other gathering hypothetical proteins with different function or no other known. Comparative modelling of the strongest candidates provides support for the hypothesis that these proteins may give rise to structurally viable cyclic nucleotide binding domains. Pull-down and nucleotide displacement assays strongly suggest that TcCLB.508523.80 could bind cAMP and eventually be a new putative PKA-independent cAMP effector in T. cruzi. © 2015 Elsevier B.V.

Registro:

Documento: Artículo
Título:Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi
Autor:Jäger, A.V.; De Gaudenzi, J.G.; Mild, J.G.; Cormack, B.M.; Pantano, S.; Altschuler, D.L.; Edreira, M.M.
Filiación:Instituto de Investigaciones Biotecnológicas, Instituto Tecnológico de Chascomús, UNSAM-CONICET, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad de Buenos Aires, 1428, Argentina
IQUIBICEN-CONICET, Ciudad de Buenos Aires, Argentina
Institut Pasteur de Montevideo, Uruguay
Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, United States
Palabras clave:cAMP binding proteins; cAMP novel effectors; cAMP signaling; Trypanosoma cruzi; cyclic AMP binding protein; cyclic AMP dependent protein kinase catalytic subunit; proteome; protozoal protein; carrier protein; cyclic nucleotide; protein binding; protozoal protein; amino acid sequence; Article; computer model; controlled study; gene segregation; molecular model; molecular phylogeny; nonhuman; priority journal; protein analysis; protein expression; protein function; protein localization; proteomics; sequence analysis; Trypanosoma cruzi; biology; cluster analysis; genetics; metabolism; phylogeny; sequence homology; Trypanosoma cruzi; Eukaryota; Protozoa; Trypanosoma cruzi; Carrier Proteins; Cluster Analysis; Computational Biology; Nucleotides, Cyclic; Phylogeny; Protein Binding; Protozoan Proteins; Sequence Homology, Amino Acid; Trypanosoma cruzi
Año:2014
Volumen:198
Número:2
Página de inicio:104
Página de fin:112
DOI: http://dx.doi.org/10.1016/j.molbiopara.2015.02.002
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:carrier protein, 80700-39-6; Carrier Proteins; Nucleotides, Cyclic; Protozoan Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v198_n2_p104_Jager

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Citas:

---------- APA ----------
Jäger, A.V., De Gaudenzi, J.G., Mild, J.G., Cormack, B.M., Pantano, S., Altschuler, D.L. & Edreira, M.M. (2014) . Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi. Molecular and Biochemical Parasitology, 198(2), 104-112.
http://dx.doi.org/10.1016/j.molbiopara.2015.02.002
---------- CHICAGO ----------
Jäger, A.V., De Gaudenzi, J.G., Mild, J.G., Cormack, B.M., Pantano, S., Altschuler, D.L., et al. "Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi" . Molecular and Biochemical Parasitology 198, no. 2 (2014) : 104-112.
http://dx.doi.org/10.1016/j.molbiopara.2015.02.002
---------- MLA ----------
Jäger, A.V., De Gaudenzi, J.G., Mild, J.G., Cormack, B.M., Pantano, S., Altschuler, D.L., et al. "Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 198, no. 2, 2014, pp. 104-112.
http://dx.doi.org/10.1016/j.molbiopara.2015.02.002
---------- VANCOUVER ----------
Jäger, A.V., De Gaudenzi, J.G., Mild, J.G., Cormack, B.M., Pantano, S., Altschuler, D.L., et al. Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi. Mol. Biochem. Parasitol. 2014;198(2):104-112.
http://dx.doi.org/10.1016/j.molbiopara.2015.02.002