Abstract:
A recent addition to the arsenal of tools for glycome analysis is the use of metabolic labels that allow covalent tagging of glycans with imaging probes. In this work we show that N-azidoglucosamine was successfully incorporated into glycolipidic structures of Plasmodium falciparum intraerythrocytic stages. The ability to tag glycoconjugates selectively with a fluorescent reporter group permits TLC detection of the glycolipids providing a new method to quantify dynamic changes in the glycosylation pattern and facilitating direct mass spectrometry analyses. Presence of glycosylphosphatidylinositol and glycosphingolipid structures was determined in the different extracts. Furthermore, the fluorescent tag was used as internal matrix for the MALDI experiment making even easier the analysis. © 2012 Elsevier B.V.
Registro:
Documento: |
Artículo
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Título: | Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry |
Autor: | Piñero, T.; Peres, V.J.; Katzin, A.; Couto, A.S. |
Filiación: | CIHIDECAR, Departamento de Química Orgánica, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina Departamento de Parasitología, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil
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Palabras clave: | Glycolipids; Metabolic oligosaccharide engineering; P. falciparum; glucosamine derivative; glycolipid; glycosphingolipid; glycosylphosphatidylinositol; n azidoglucosamine; oligosaccharide; unclassified drug; article; erythrocyte; fluorescence analysis; glycosylation; mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; Plasmodium falciparum; priority journal; structure analysis; thin layer chromatography; ultraviolet radiation; Chromatography, Thin Layer; Erythrocytes; Fluorescent Dyes; Glycolipids; Glycosphingolipids; Glycosylation; Glycosylphosphatidylinositols; Mass Spectrometry; Metabolic Engineering; Oligosaccharides; Plasmodium falciparum; Staining and Labeling; Plasmodium falciparum |
Año: | 2012
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Volumen: | 182
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Número: | 1-2
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Página de inicio: | 88
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Página de fin: | 92
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DOI: |
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008 |
Título revista: | Molecular and Biochemical Parasitology
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Título revista abreviado: | Mol. Biochem. Parasitol.
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ISSN: | 01666851
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CODEN: | MBIPD
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CAS: | Fluorescent Dyes; Glycolipids; Glycosphingolipids; Glycosylphosphatidylinositols; Oligosaccharides
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v182_n1-2_p88_Pinero |
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Citas:
---------- APA ----------
Piñero, T., Peres, V.J., Katzin, A. & Couto, A.S.
(2012)
. Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry. Molecular and Biochemical Parasitology, 182(1-2), 88-92.
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008---------- CHICAGO ----------
Piñero, T., Peres, V.J., Katzin, A., Couto, A.S.
"Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry"
. Molecular and Biochemical Parasitology 182, no. 1-2
(2012) : 88-92.
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008---------- MLA ----------
Piñero, T., Peres, V.J., Katzin, A., Couto, A.S.
"Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry"
. Molecular and Biochemical Parasitology, vol. 182, no. 1-2, 2012, pp. 88-92.
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008---------- VANCOUVER ----------
Piñero, T., Peres, V.J., Katzin, A., Couto, A.S. Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry. Mol. Biochem. Parasitol. 2012;182(1-2):88-92.
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008