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Abstract:

Calmodulin has been purified from Trypanosoma cruzi epimastigote forms by ion-exchange chromatography, gel filtration and affinity chromatography on 2-chloro-10-(3-aminopropyl)phenotiazine-Sepharose. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the factor showed a polypeptide band with an apparent molecular weight of 16 000. In addition, cyclic AMP phosphodiesterase activity from T. cruzi epimastigote forms was purified by ion-exchange chromatography and affinity chromatography on a brain calmodulin-Sepharose column. The enzyme was activated by homologous calmodulin as well as by bovine brain and Neurospora crassa calmodulins. The activation required micromolar concentrations of Ca2+ and it was blocked by EGTA and by some neuroleptic drugs such as chlorpromazine, fluphenazine and compound 48 80. Activations were observed at micromolar concentrations of cyclic AMP as substrate. In addition, T. cruzi calmodulin was also active in bringing about the stimulation of brain phosphodiesterase. © 1985.

Registro:

Documento: Artículo
Título:Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi
Autor:Téllez-iñón, M.T.; Ulloa, R.M.; Torruella, M.; Torres, H.N.
Filiación:Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Instituto de Investigaciones Bioquímicas, Facultad de Ciencías Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Calcium; Calmodulin; Cyclic AMP; Phosphodiesterase; Trypanosoma cruzi; adenosine phosphate; calcium; calmodulin; enzyme; phosphodiesterase; 3',5' cyclic nucleotide phosphodiesterase; calcium; calmodulin; animal cell; metabolism; nonhuman; priority journal; protozoon; trypanosoma cruzi; animal; article; brain cortex; cattle; comparative study; enzymology; isolation and purification; kinetics; Neurospora crassa; Trypanosoma cruzi; 3',5'-Cyclic-Nucleotide Phosphodiesterase; Animal; Calcium; Calmodulin; Cattle; Cerebral Cortex; Comparative Study; Kinetics; Neurospora crassa; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1985
Volumen:17
Número:2
Página de inicio:143
Página de fin:153
DOI: http://dx.doi.org/10.1016/0166-6851(85)90013-1
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:adenosine phosphate, 61-19-8, 8063-98-7; calcium, 7440-70-2; 3',5' cyclic nucleotide phosphodiesterase, 9040-59-9; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Calcium, 7440-70-2; Calmodulin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v17_n2_p143_Tellezinon

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Citas:

---------- APA ----------
Téllez-iñón, M.T., Ulloa, R.M., Torruella, M. & Torres, H.N. (1985) . Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi. Molecular and Biochemical Parasitology, 17(2), 143-153.
http://dx.doi.org/10.1016/0166-6851(85)90013-1
---------- CHICAGO ----------
Téllez-iñón, M.T., Ulloa, R.M., Torruella, M., Torres, H.N. "Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi" . Molecular and Biochemical Parasitology 17, no. 2 (1985) : 143-153.
http://dx.doi.org/10.1016/0166-6851(85)90013-1
---------- MLA ----------
Téllez-iñón, M.T., Ulloa, R.M., Torruella, M., Torres, H.N. "Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 17, no. 2, 1985, pp. 143-153.
http://dx.doi.org/10.1016/0166-6851(85)90013-1
---------- VANCOUVER ----------
Téllez-iñón, M.T., Ulloa, R.M., Torruella, M., Torres, H.N. Calmodulin and Ca2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi. Mol. Biochem. Parasitol. 1985;17(2):143-153.
http://dx.doi.org/10.1016/0166-6851(85)90013-1