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Abstract:

In animal cells, the exon junction complex (EJC) is deposited onto mRNAs during the second step of splicing, 20-24 nt upstream of the exon-exon junction. The EJC core contains four proteins: Mago, Y14, eIF4AIII and Btz. In trypanosomes, cis-splicing is very rare but all mRNAs are subject to 5′ trans-splicing of a 39-nt RNA sequence. Here we show that trypanosomes have a conserved Mago and a divergent Y14 protein, but we were unable to identify a Btz orthologue. We demonstrate that Mago and Y14 form a stable heterodimer using yeast two hybrid analyses. We also show that this complex co-purifies in vivo in trypanosomes with a protein containing an NTF2 domain, typically involved in mRNA transport. © 2009 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Identification of core components of the exon junction complex in trypanosomes
Autor:Bercovich, N.; Levin, M.J.; Clayton, C.; Vazquez, M.P.
Filiación:INGEBI-CONICET, Vuelta de Obligado 2490, 2P, Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, Argentina
Zentrum für Molekulare Biologie, Heidelberg Universität, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
Palabras clave:EJC; Mago; mRNA export; Trypanosomes; Y14; heterodimer; messenger RNA; protozoal protein; article; evolutionary homology; exon; in vivo study; nonhuman; nucleotide sequence; priority journal; protein domain; RNA sequence; RNA splicing; trans splicing; Trypanosoma; yeast; Amino Acid Sequence; Animals; Exons; Humans; Molecular Sequence Data; Protein Binding; Protozoan Proteins; RNA Splicing; RNA Transport; RNA-Binding Proteins; Sequence Alignment; Trypanosoma brucei brucei; Trypanosoma cruzi; Trypanosomiasis; Animalia; Mago
Año:2009
Volumen:166
Número:2
Página de inicio:190
Página de fin:193
DOI: http://dx.doi.org/10.1016/j.molbiopara.2009.03.008
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:Protozoan Proteins; RNA-Binding Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v166_n2_p190_Bercovich

Referencias:

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Citas:

---------- APA ----------
Bercovich, N., Levin, M.J., Clayton, C. & Vazquez, M.P. (2009) . Identification of core components of the exon junction complex in trypanosomes. Molecular and Biochemical Parasitology, 166(2), 190-193.
http://dx.doi.org/10.1016/j.molbiopara.2009.03.008
---------- CHICAGO ----------
Bercovich, N., Levin, M.J., Clayton, C., Vazquez, M.P. "Identification of core components of the exon junction complex in trypanosomes" . Molecular and Biochemical Parasitology 166, no. 2 (2009) : 190-193.
http://dx.doi.org/10.1016/j.molbiopara.2009.03.008
---------- MLA ----------
Bercovich, N., Levin, M.J., Clayton, C., Vazquez, M.P. "Identification of core components of the exon junction complex in trypanosomes" . Molecular and Biochemical Parasitology, vol. 166, no. 2, 2009, pp. 190-193.
http://dx.doi.org/10.1016/j.molbiopara.2009.03.008
---------- VANCOUVER ----------
Bercovich, N., Levin, M.J., Clayton, C., Vazquez, M.P. Identification of core components of the exon junction complex in trypanosomes. Mol. Biochem. Parasitol. 2009;166(2):190-193.
http://dx.doi.org/10.1016/j.molbiopara.2009.03.008