Artículo

Salto, M.L.; Kuhlenschmidt, T.; Kuhlenschmidt, M.; de Lederkremer, R.M.; Docampo, R. "Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi" (2008) Molecular and Biochemical Parasitology. 158(2):120-130
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Abstract:

Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [3H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition. © 2007 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
Autor:Salto, M.L.; Kuhlenschmidt, T.; Kuhlenschmidt, M.; de Lederkremer, R.M.; Docampo, R.
Filiación:Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61802, United States
CIHIDECAR, Departmento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Buenos Aires, 1428, Argentina
Center for Tropical and Emerging Global Diseases, Department of Cellular Biology, University of Georgia, Athens, GA 30602, United States
Palabras clave:Acidocalcisomes; Glycolipids; Phospholipids; Trypanosoma cruzi; 3beta hydroxysterol derivative; glycolipid; phosphatidylcholine; phosphatidylethanolamine; phosphatidylinositol; phospholipid; sterol derivative; unclassified drug; article; controlled study; electrospray mass spectrometry; epimastigote; lipid analysis; lipid composition; nonhuman; priority journal; Trypanosoma cruzi; Animals; Calcium; Centrifugation, Density Gradient; Glycolipids; Hydrogen-Ion Concentration; Organelles; Phospholipids; Spectrometry, Mass, Electrospray Ionization; Trypanosoma cruzi; Trypanosoma cruzi
Año:2008
Volumen:158
Número:2
Página de inicio:120
Página de fin:130
DOI: http://dx.doi.org/10.1016/j.molbiopara.2007.12.001
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:phosphatidylcholine, 55128-59-1, 8002-43-5; phosphatidylethanolamine, 1405-71-6; Calcium, 7440-70-2; Glycolipids; Phospholipids
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v158_n2_p120_Salto

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Citas:

---------- APA ----------
Salto, M.L., Kuhlenschmidt, T., Kuhlenschmidt, M., de Lederkremer, R.M. & Docampo, R. (2008) . Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi. Molecular and Biochemical Parasitology, 158(2), 120-130.
http://dx.doi.org/10.1016/j.molbiopara.2007.12.001
---------- CHICAGO ----------
Salto, M.L., Kuhlenschmidt, T., Kuhlenschmidt, M., de Lederkremer, R.M., Docampo, R. "Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi" . Molecular and Biochemical Parasitology 158, no. 2 (2008) : 120-130.
http://dx.doi.org/10.1016/j.molbiopara.2007.12.001
---------- MLA ----------
Salto, M.L., Kuhlenschmidt, T., Kuhlenschmidt, M., de Lederkremer, R.M., Docampo, R. "Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 158, no. 2, 2008, pp. 120-130.
http://dx.doi.org/10.1016/j.molbiopara.2007.12.001
---------- VANCOUVER ----------
Salto, M.L., Kuhlenschmidt, T., Kuhlenschmidt, M., de Lederkremer, R.M., Docampo, R. Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi. Mol. Biochem. Parasitol. 2008;158(2):120-130.
http://dx.doi.org/10.1016/j.molbiopara.2007.12.001