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Abstract:

Parvulins are a conserved group of peptidyl-prolyl cis/trans isomerases (PPIases) that catalyze the cis/trans isomerization of proline-preceding peptide bonds. Parvulin-class PPIases are structurally unrelated to cyclophilins and FK506-binding proteins that are defined as receptors for immunosuppressive drugs. In Trypanosoma cruzi we identified parvulin TcPIN1 as a homolog of the human hPin1 PPIase. The 117 amino acids of the TcPIN1 display 40% identity with the catalytic core of hPin1 and exhibit prolyl cis/trans isomerase activity. TcPIN1 lacks the WW domain at the N-terminus, and is able to rescue the temperature-sensitive phenotype on a mutation in the Saccharomyces cerevisiae hPin1 homolog, ESS1/PTF1. Western blot analysis revealed that the enzyme was present both in dividing and non-dividing forms of T. cruzi. In epimastigote cells neither cell growth kinetics nor cell morphology was affected by the overexpression of the small parvulin TcPIN1. These results suggest the occurrence of a supplementary conserved level of post-translational control in trypanosomatids. © 2007 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae
Autor:Erben, E.D.; Daum, S.; Téllez-Iñón, M.T.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120 Halle, Germany
Palabras clave:Cell cycle; Parvulin; Pin1; Prolyl cis/trans isomerization; Trypanosoma cruzi; Trypanosomatids; amino acid derivative; peptidylprolyl isomerase Pin1; protein parvulin; Saccharomyces cerevisiae protein; unclassified drug; amino terminal sequence; animal cell; article; catalysis; cell structure; gene mutation; isomerization; nonhuman; phenotype; priority journal; protein analysis; temperature; Trypanosoma cruzi; Western blotting; Amino Acid Sequence; Animals; Cell Cycle; Gene Expression Regulation; Genetic Complementation Test; Humans; Molecular Sequence Data; Peptidylprolyl Isomerase; Protozoan Proteins; Recombinant Proteins; Sequence Alignment; Sequence Analysis, DNA; Trypanosoma cruzi; Saccharomyces cerevisiae; Trypanosoma cruzi; Trypanosomatidae
Año:2007
Volumen:153
Número:2
Página de inicio:186
Página de fin:193
DOI: http://dx.doi.org/10.1016/j.molbiopara.2007.03.004
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:NIMA-interacting peptidylprolyl isomerase; Peptidylprolyl Isomerase, 5.2.1.8; Protozoan Proteins; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v153_n2_p186_Erben

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Citas:

---------- APA ----------
Erben, E.D., Daum, S. & Téllez-Iñón, M.T. (2007) . The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae. Molecular and Biochemical Parasitology, 153(2), 186-193.
http://dx.doi.org/10.1016/j.molbiopara.2007.03.004
---------- CHICAGO ----------
Erben, E.D., Daum, S., Téllez-Iñón, M.T. "The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae" . Molecular and Biochemical Parasitology 153, no. 2 (2007) : 186-193.
http://dx.doi.org/10.1016/j.molbiopara.2007.03.004
---------- MLA ----------
Erben, E.D., Daum, S., Téllez-Iñón, M.T. "The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae" . Molecular and Biochemical Parasitology, vol. 153, no. 2, 2007, pp. 186-193.
http://dx.doi.org/10.1016/j.molbiopara.2007.03.004
---------- VANCOUVER ----------
Erben, E.D., Daum, S., Téllez-Iñón, M.T. The Trypanosoma cruzi PIN1 gene encodes a parvulin peptidyl-prolyl cis/trans isomerase able to replace the essential ESS1 in Saccharomyces cerevisiae. Mol. Biochem. Parasitol. 2007;153(2):186-193.
http://dx.doi.org/10.1016/j.molbiopara.2007.03.004