Artículo

Muñoz, M.J.; Santori, M.I.; Rojas, F.; Gómez, E.B.; Téllez-Iñón, M.T. "Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant" (2006) Molecular and Biochemical Parasitology. 147(2):154-162
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Abstract:

The complex mechanism of cell division in trypanosomatids is not completely fully understood. CRKs (cdc2-related kinases), Cyclins and CKSs (cdc2-kinase subunit) are involved in the progression through the cell cycle. The CKS proteins were first described as components of the cell cycle machinery in yeast and their action has been implicated in the regulation of CDK function. In the present work we identified Tcp12CKS1 a member of the CKS family in the parasite Trypanosoma cruzi. TcCKS1 is expressed in the three forms of T. cruzi. By using anti-Tcp12CKS1 antiserum, protein kinase (PK) activities were immunoprecipitated. The PK activity level varies depending on the stage analyzed, being lower in trypomastigotes and thus suggesting that different stages have different CKS-CRK complexes. Moreover, these PK activities were inhibited by using Flavopiridol, a known CDKs inhibitor. Western blot analyses demonstrated that in the epimastigote stage, p12CKS1 stably interacts with TcCRK1 and TcCRK3. In addition, Tcp12CKS1 was able to rescue the p13SUC1 null mutant of S. pombe. The functional complementation between the CKS proteins of two evolutionary distant organisms supports the role of Tcp12CKS1 as a key regulator in T. cruzi cell cycle. © 2006 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant
Autor:Muñoz, M.J.; Santori, M.I.; Rojas, F.; Gómez, E.B.; Téllez-Iñón, M.T.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Cell cycle; CKS; CRKs; Cyclins; Trypanosoma cruzi; antiserum; cyclin dependent kinase; cyclin dependent kinase 1; cyclin dependent kinase inhibitor; cycline; protein kinase; protozoal protein; Schizosaccharomyces pombe protein; cell cycle protein; CRK protein, protozoan; protein kinase; Suc1 protein, S pombe; article; cell cycle; enzyme activity; enzyme inhibition; epimastigote; immunoprecipitation; nonhuman; nucleotide sequence; priority journal; protein analysis; protein interaction; Schizosaccharomyces pombe; Trypanosoma cruzi; trypomastigote; Western blotting; yeast; amino acid sequence; animal; chemistry; DNA sequence; enzymology; gene expression regulation; genetics; growth, development and aging; metabolism; molecular genetics; mutation; Schizosaccharomyces; Schizosaccharomyces pombe; Trypanosoma cruzi; Trypanosomatidae; Amino Acid Sequence; Animals; CDC2 Protein Kinase; Cell Cycle Proteins; Gene Expression Regulation; Molecular Sequence Data; Mutation; Protein Kinases; Protozoan Proteins; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Sequence Analysis, DNA; Trypanosoma cruzi
Año:2006
Volumen:147
Número:2
Página de inicio:154
Página de fin:162
DOI: http://dx.doi.org/10.1016/j.molbiopara.2006.02.006
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:cyclin dependent kinase, 150428-23-2; protein kinase, 9026-43-1; CDC2 Protein Kinase, 2.7.1.37; CRK protein, protozoan; Cell Cycle Proteins; Protein Kinases, 2.7.1.37; Protozoan Proteins; Schizosaccharomyces pombe Proteins; Suc1 protein, S pombe
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v147_n2_p154_Munoz

Referencias:

  • De Souza, W., Cell biology of Trypanosoma cruzi (1984) Int Rev Cytol, 86, pp. 197-283
  • Tyler, K.M., Engman, D.M., The life cycle of Trypanosoma cruzi revisited (2001) Int J Parasitol, 31, pp. 472-481
  • Morgan, D., Cyclin-dependent kinases: engines, clocks and microprocessors (1997) Annu Cell Dev Biol, 13, pp. 261-291
  • Dacks, J.B., Doolittle, W.F., Reconstructing/deconstructing the earliest eukaryotes: how comparative genomics can help (2001) Cell, 107, pp. 419-425
  • Mottram, J.C., Smith, G., A family of trypanosome Cdc2p-related protein kinases (1995) Gene, 162, pp. 147-152
  • Hammarton, T.C., Mottram, J.C., Doerig, C., The cell cycle of parasitic protozoa: potential for chemotherapeutic exploitation (2003) Progr Cell Cycle Res, 5, pp. 91-101
  • Hayles, J., Beach, D., Durkacz, B., Nurse, P., The fission yeast cell cycle control gene cdc2: isolation of a sequence suc1 that suppresses cdc2 mutant function (1986) Mol Gen Genet, 202, pp. 291-293
  • Harper, J.W., Adapting roles for Cks proteins (2001) Curr Biol, 11, pp. 431-435
  • Patra, D., Wang, S.X., Kumagai, A., Dunphy, W.G., The Xenopus Suc1/Cks protein promotes the phosphorylation of G2/M regulators (1999) J Biol Chem, 274, pp. 36839-38342
  • Patra, D., Dunphy, W.G., Xe-p9, a Xenopus Suc1/Cks protein, is essential for the Cdc2-dependent phosphorylation of the anaphase- promoting complex at mitosis (1998) Genes Dev, 12, pp. 2549-2559
  • Kaiser, P., Moncollin, V., Clarke, D.J., Cyclin-dependent kinase and Cks/Suc1 interact with the proteasome in yeast to control proteolysis of M-phase targets (1999) Genes Dev, 13, pp. 1190-1202
  • Polinko, E.S., Strome, S., Depletion of a Cks homolog in C. elegans embryos uncovers a post-metaphase role in meiosis and mitosis (2000) Curr Biol, 10, pp. 1471-1474
  • Nakayama, K., Nagahama, H., Minamishima, Y.A., Targeted disruption of Skp2 results in accumulation of cyclin E and p27(Kip1), polyploidy and centrosome overduplication (2000) EMBO J, 19, pp. 2069-2081
  • Pines, J., Cell Cycle: reaching for a role for CKS proteins (1996) Curr Biol, 6, pp. 1399-1402
  • Gómez, E.B., Kornblihtt, A.R., Téllez-Iñón, M.T., Cloning of a cdc2-related protein kinase from the parasite Trypanosoma cruzi that interacts with mammalian cyclins (1998) Mol Biochem Parasitol, 91, pp. 337-351
  • Gómez, E.B., Santori, M.I., Laría, S., Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins (2001) Mol Biochem Parasitol, 113, pp. 97-108
  • Andrews, N., Colli, W., Adhesion and interiorization of Trypanosoma cruzi in mammalian cells (1982) J Protozool, 29, pp. 264-269
  • Da Rocha, W.D., Silva, R.A., Bartholomeu, D.C., Pires, S.F., Freitas, J.M., Macedo, A.M., Expression of exogenous genes in Trypanosoma cruzi: improving vectors and electroporation protocols (2004) Parasitol Res, 92, pp. 113-120
  • Siam, R., Dolan, W.P., Forsburg, S.L., Choosing and using Schizosaccaromyces pombe plasmids (2004) Methods, 33, pp. 189-198
  • Basi, G., Schmid, E., Maundrell, K., TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility (1993) Gene, 123, pp. 131-136
  • Mottram, J.C., Grant, K., Leishmania mexicana p12cks1, a homologue of fission yeast p13suc1, associates with a stage-regulated histone H1 kinase (1996) Biochem J, 316, pp. 833-839
  • Hammarton, T.C., Clark, J., Douglas, F., Boshart, M., Mottram, J.C., Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin (2003) J Biol Chem, 278, pp. 22877-22886
  • Bourne, Y., Arvai, A.S., Bernstein, S.L., Crystal structure of the cell cycle-regulatory protein suc1 reveals a beta-hinge conformational switch (1995) Proc Natl Acad Sci, 92, pp. 10232-10236
  • Parge, H.E., Arvai, A.S., Murtari, D.J., Reed, S.I., Tainer, J.A., Human CksHs2 atomic structure: a role for its hexameric assembly in cell cycle control (1993) Science, 262, pp. 387-395
  • Kim, Y., Ohyama, H., Patel, V., Figueiredo, M., Wong, D.T., Mutation of Cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect (2005) J Biol Chem, 280, pp. 23273-23279
  • Santori, M.I., Laria, S., Gómez, E.B., Espinosa, I., Galanti, N., Téllez-Iñón, M.T., Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi (2002) Mol Biochem Parasitol, 121, pp. 225-232
  • Basi, G., Draetta, G., p13SUC1 of Schizosaccharomyces pombe regulates two distinct forms of the mitotic cdc2 kinase (1995) Mol Cell Biol, 15, pp. 2028-2036
  • Ducommun, B., Brambilla, P., Draetta, G., Mutations at sites involved in Suc1 binding inactivate Cdc2 (1991) Mol Cell Biol, 11, pp. 6177-6184
  • Bourne, Y., Watson, M.H., Hickey, M.J., Crystal structure and mutational analysis of the human CDK2 kinase complex with cell cycle regulatory protein CksHs1 (1996) Cell, 84, pp. 863-874
  • Natarajan, S.K., Stern, L.J., Sadegh-Nasseri, S., Sodium dodecylsulfate stability of HLA-DR1 complexes correlates with burial of hydrophobic residues in pocket1 (1999) J Immunol, 162, pp. 3463-3470
  • Morris, M.C., HeiTc, F., Divita, G., Kinetics of dimerization and interactions of p13 suc1 with cyclin-dependent kinases (1998) Biochemistry, 37, pp. 14257-14266
  • Sitry, D., Seeliger, M.A., Ko, T.K., Three different binding sites of Cks1 are requiered for p27-Ubiquitin ligation (2002) J Biol Chem, 277, pp. 42233-42240

Citas:

---------- APA ----------
Muñoz, M.J., Santori, M.I., Rojas, F., Gómez, E.B. & Téllez-Iñón, M.T. (2006) . Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant. Molecular and Biochemical Parasitology, 147(2), 154-162.
http://dx.doi.org/10.1016/j.molbiopara.2006.02.006
---------- CHICAGO ----------
Muñoz, M.J., Santori, M.I., Rojas, F., Gómez, E.B., Téllez-Iñón, M.T. "Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant" . Molecular and Biochemical Parasitology 147, no. 2 (2006) : 154-162.
http://dx.doi.org/10.1016/j.molbiopara.2006.02.006
---------- MLA ----------
Muñoz, M.J., Santori, M.I., Rojas, F., Gómez, E.B., Téllez-Iñón, M.T. "Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant" . Molecular and Biochemical Parasitology, vol. 147, no. 2, 2006, pp. 154-162.
http://dx.doi.org/10.1016/j.molbiopara.2006.02.006
---------- VANCOUVER ----------
Muñoz, M.J., Santori, M.I., Rojas, F., Gómez, E.B., Téllez-Iñón, M.T. Trypanosoma cruzi Tcp12CKS1 interacts with parasite CRKs and rescues the p13SUC1 fission yeast mutant. Mol. Biochem. Parasitol. 2006;147(2):154-162.
http://dx.doi.org/10.1016/j.molbiopara.2006.02.006