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Abstract:

VIPER was initially characterized as a 2326 bp LTR-like retroelement associated to SIRE, a short interspersed repetitive element specific of Trypanosoma cruzi. It carried a single ORF that coded for a putative reverse transcriptase-RNAse H protein, suggesting that it could be a truncated copy of a longer retroelement. Herein we report the identification and characterization of a complete 4480 bp long VIPER in the T. cruzi genome. The complete VIPER harbored three non-overlapped domains encoding for a GAG-like, a tyrosine recombinase and a reverse transcriptase-RNAse H proteins. VIPER elements were also found in the genomes of Trypanosoma brucei and Trypanosoma vivax, but not in Leishmania sp. On the basis of its reverse transcriptase phylogeny, VIPER was classified as an LTR retroelement. However, VIPER was structurally related to the tyrosine recombinase encoding retroelements, DIRS and Ngaro. Phylogenetic analysis showed that VIPER's tyrosine recombinase grouped with the transposases RCI1 of Escherichia coli and Ye24 and Ye72 of Haemophilus influenzae within a major branch of prokaryotic recombinases. Taken together, VIPER's structure, the nature of its tyrosine recombinase, the unique features of its reverse transcriptase catalytic consensus motif and the fact that it was found in Trypanosomes, an early branching eukaryote, suggest that VIPER may be the closest relative of the founder element of the tyrosine recombinase encoding retrotransposons known up to date. Our analysis revealed that tyrosine recombinase-encoding retroelements were originated as early in evolution as non-LTR retroelements and suggests that VIPER, Ngaro and DIRS elements may constitute a third group of retrotransposons, distinct from both LTR and non-LTR retroelements. © 2005 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:The VIPER elements of trypanosomes constitute a novel group of tyrosine recombinase-enconding retrotransposons
Autor:Lorenzi, H.A.; Robledo, G.; Levin, M.J.
Filiación:Laboratorio de Biologia Molecular de la Enfermedad de Chagas (LaBMECh) INGEBI, Centro de Genomica Aplicada (CeGA), University of Buenos Aires, Vuelta de Obligado 2490 2P, 1428, Buenos Aires, Argentina
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, United States
Institut Cochin, Département Maladies Infectieuses, INSERM U567, Paris F-75014, France
Palabras clave:LTR-retrotransposon; Trypanosomes; Tyrosine recombinase; Gag protein; recombinase; ribonuclease H; RNA directed DNA polymerase; tyrosine; article; controlled study; Escherichia coli; genome; Haemophilus influenzae; nonhuman; nucleotide sequence; phylogeny; priority journal; prokaryote; retroposon; Trypanosoma brucei; Trypanosoma cruzi; Trypanosoma vivax; unindexed sequence; Escherichia coli; Eukaryota; Haemophilus influenzae; Leishmania sp.; Prokaryota; Trypanosoma brucei; Trypanosoma cruzi; Trypanosoma vivax
Año:2006
Volumen:145
Número:2
Página de inicio:184
Página de fin:194
DOI: http://dx.doi.org/10.1016/j.molbiopara.2005.10.002
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:ribonuclease H, 9050-76-4; RNA directed DNA polymerase, 37213-50-6, 9068-38-6; tyrosine, 16870-43-2, 55520-40-6, 60-18-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v145_n2_p184_Lorenzi

Referencias:

  • Kazazian Jr., H.H., Mobile elements: Drivers of genome evolution (2004) Science, 303 (5664), pp. 1626-1632
  • Xiong, Y., Eickbush, T.H., Origin and evolution of retroelements based upon their reverse transcriptase sequences (1990) EMBO J, 9 (10), pp. 3353-3362
  • Goodwin, T.J., Poulter, R.T., A new group oftyrosine recombinase-encoding retrotransposons (2004) Mol Biol Evol, 21 (4), pp. 746-759
  • Goodwin, T.J., Poulter, R.T., The DIRS1 group of retrotransposons (2001) Mol Biol Evol, 18 (11), pp. 2067-2082
  • Boeke, J.D., The unusual phylogenetic distribution of retrotransposons: A hypothesis (2003) Genome Res, 13 (9), pp. 1975-1983
  • Andersson, B., Complete sequence of a 93.4-kb contig from chromosome 3 of Trypanosoma cruzi containing a strand-switch region (1998) Genome Res, 8 (8), pp. 809-816
  • Malik, H.S., Eickbush, T.H., Phylogenetic analysis of ribonuclease H domains suggests a late, chimeric origin of LTR retrotransposable elements and retroviruses (2001) Genome Res, 11 (7), pp. 1187-1197
  • Requena, J.M., Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3′-end of a poly(A)+ transcript (1994) Gene, 146 (2), pp. 245-250
  • Martin, F., Characterization of a non-long terminal repeat retrotransposon cDNA (L1Tc) from Trypanosoma cruzi: Homology of the first ORF with the ape family of DNA repair enzymes (1995) J Mol Biol, 247 (1), pp. 49-59
  • Kimmel, B.E., Ole-Moiyoi, O.K., Young, J.R., Ingi, a 5.2-kb dispersed sequence element from Trypanosoma brucei that carries half of a smaller mobile element at either end and has homology with mammalian LINEs (1987) Mol Cell Biol, 7 (4), pp. 1465-1475
  • Villanueva, M.S., A new member of a family of site-specific retrotransposons is present in the spliced leader RNA genes of Trypanosoma cruzi (1991) Mol Cell Biol, 11 (12), pp. 6139-6148
  • Vazquez, M., The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons (2000) Proc Natl Acad Sci USA, 97 (5), pp. 2128-2133
  • Vazquez, M., Analysis of the distribution of SIRE in the nuclear genome of Trypanosoma cruzi (1999) Gene, 239 (2), pp. 207-216
  • Ghedin, E., Gene synteny and evolution of genome architecture in trypanosomatids (2004) Mol Biochem Parasitol, 134 (2), pp. 183-191
  • Orlinsky, K.J., Mutations in the Ty3 major homology region affect multiple steps in Ty3 retrotransposition (1996) J Virol, 70 (6), pp. 3440-3448
  • Copeland, C.S., Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni (2003) J Virol, 77 (11), pp. 6153-6166
  • Gorelick, R.J., Point mutants of Moloney murine leukemia virus that fail to package viral RNA: Evidence for specific RNA recognition by a "zinc finger-like" protein sequence (1988) Proc Natl Acad Sci USA, 85 (22), pp. 8420-8424
  • Muriaux, D., Role of murine leukemia virus nucleocapsid protein in virus assembly (2004) J Virol, 78 (22), pp. 12378-12385
  • Nunes-Duby, S.E., Similarities and differences among 105 members of the Int family of site-specific recombinases (1998) Nucleic Acids Res, 26 (2), pp. 391-406
  • Esposito, D., Scocca, J.J., The integrase family oftyrosine recombinases: Evolution of a conserved active site domain (1997) Nucleic Acids Res, 25 (18), pp. 3605-3614
  • Kwon, H.J., Flexibility in DNA recombination: Structure of the lambda integrase catalytic core (1997) Science, 276 (5309), pp. 126-131
  • Poch, O., Identification of four conserved motifs among the RNA-dependent polymerase encoding elements (1989) EMBO J, 8 (12), pp. 3867-3874
  • Vazquez, M.P., Schijman, A.G., Levin, M.J., A short interspersed repetitive element provides a new 3′-acceptor site for trans-splicing in certain ribosomal P2 beta protein genes of Trypanosoma cruzi (1994) Mol Biochem Parasitol, 64 (2), pp. 327-336
  • Bringaud, F., Identification of non-autonomous non-LTR retrotransposons in the genome of Trypanosoma cruzi (2002) Mol Biochem Parasitol, 124 (1-2), pp. 73-78
  • Bringaud, F., The ingi and RIME non-LTR retrotransposons are not randomly distributed in the genome of Trypanosoma brucei (2004) Mol Biol Evol, 21 (3), pp. 520-528
  • El-Sayed, N.M., The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease (2005) Science, 309 (5733), pp. 409-415
  • Shi, H., Argonaute protein in the early divergent eukaryote Trypanosoma brucei: Control of small interfering RNA accumulation and retroposon transcript abundance (2004) Mol Cell Biol, 24 (1), pp. 420-427
  • Darocha, W.D., Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi (2004) Mol Biochem Parasitol, 133 (2), pp. 175-186

Citas:

---------- APA ----------
Lorenzi, H.A., Robledo, G. & Levin, M.J. (2006) . The VIPER elements of trypanosomes constitute a novel group of tyrosine recombinase-enconding retrotransposons. Molecular and Biochemical Parasitology, 145(2), 184-194.
http://dx.doi.org/10.1016/j.molbiopara.2005.10.002
---------- CHICAGO ----------
Lorenzi, H.A., Robledo, G., Levin, M.J. "The VIPER elements of trypanosomes constitute a novel group of tyrosine recombinase-enconding retrotransposons" . Molecular and Biochemical Parasitology 145, no. 2 (2006) : 184-194.
http://dx.doi.org/10.1016/j.molbiopara.2005.10.002
---------- MLA ----------
Lorenzi, H.A., Robledo, G., Levin, M.J. "The VIPER elements of trypanosomes constitute a novel group of tyrosine recombinase-enconding retrotransposons" . Molecular and Biochemical Parasitology, vol. 145, no. 2, 2006, pp. 184-194.
http://dx.doi.org/10.1016/j.molbiopara.2005.10.002
---------- VANCOUVER ----------
Lorenzi, H.A., Robledo, G., Levin, M.J. The VIPER elements of trypanosomes constitute a novel group of tyrosine recombinase-enconding retrotransposons. Mol. Biochem. Parasitol. 2006;145(2):184-194.
http://dx.doi.org/10.1016/j.molbiopara.2005.10.002