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Abstract:

Gene expression in trypanosomes is controlled mainly by post-transcriptional processes. This study was designed to analyse HX1, one of the TcP2β upstream intergenic regions. It is an efficient pre-mRNA processing region that has been widely and successfully used in Trypanosoma cruzi transfection vectors. Herein we compared its performance with other regions within the same locus, and we identified the sequence elements responsible for the HX1 efficiency in trans-splicing and protein synthesis. Our mutational analysis showed the flexibility of the branch point site selection for HX1 trans-splicing process. We demonstrated also that its 12 nt 5′UTR sequence contributes to both trans-splicing and translation efficiency. The natural insertion of the repetitive element short interspersed repetitive element (SIRE) in one of the HX1 polypyrimidine tracts decreases the translated protein level by 40%. In this report, we demonstrated that this reduction is a consequence of a decrease of five-fold in the level of processed mRNA balanced by an increased efficiency of translation due to the inclusion of a 38 nt SIRE specific sequence in the 5′UTR of the mRNA. © 2005 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi
Autor:Ben-Dov, C.P.; Levin, M.J.; Vázquez, M.P.
Filiación:Lab. Biol. Molec. Enfermedad Chagas, INGEBI-CONICET, University of Buenos Aires, Vuelta de Obligado 2490 2P, 1428 Buenos Aires, Argentina
Programme of Gene Regulation, Centre for Genomic Regulation, 08003 Barcelona, Spain
Palabras clave:Chagas disease; Post-transcriptional regulation; Repetitive sequences; trans-Splicing; messenger RNA; ribosome protein; 5' untranslated region; article; gene locus; mutational analysis; nonhuman; priority journal; protein analysis; protein synthesis; RNA processing; trans splicing; transcription regulation; Trypanosoma cruzi; insertion sequences; Trypanosoma; Trypanosoma cruzi; Trypanosoma cruzi
Año:2005
Volumen:140
Número:1
Página de inicio:97
Página de fin:105
DOI: http://dx.doi.org/10.1016/j.molbiopara.2005.01.001
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v140_n1_p97_BenDov

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Citas:

---------- APA ----------
Ben-Dov, C.P., Levin, M.J. & Vázquez, M.P. (2005) . Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi. Molecular and Biochemical Parasitology, 140(1), 97-105.
http://dx.doi.org/10.1016/j.molbiopara.2005.01.001
---------- CHICAGO ----------
Ben-Dov, C.P., Levin, M.J., Vázquez, M.P. "Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi" . Molecular and Biochemical Parasitology 140, no. 1 (2005) : 97-105.
http://dx.doi.org/10.1016/j.molbiopara.2005.01.001
---------- MLA ----------
Ben-Dov, C.P., Levin, M.J., Vázquez, M.P. "Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 140, no. 1, 2005, pp. 97-105.
http://dx.doi.org/10.1016/j.molbiopara.2005.01.001
---------- VANCOUVER ----------
Ben-Dov, C.P., Levin, M.J., Vázquez, M.P. Analysis of the highly efficient pre-mRNA processing region HX1 of Trypanosoma cruzi. Mol. Biochem. Parasitol. 2005;140(1):97-105.
http://dx.doi.org/10.1016/j.molbiopara.2005.01.001