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Abstract:

The major free glycoinositolphospholipids and protein-linked glycoinositolphospholipids in Trypanosoma cruzi contain ceramide as the lipid moiety. Ceramide was not found in mammalian glycosylphosphatidylinositol (GPI)-anchors. An alkylglycerol, either as a lyso species or acylated has been also found in T. cruzi anchors. However, unlike African trypanosomes, no diacylglycerol was detected in the GPI-anchors. Using a membrane preparation from epimastigotes upon labelling with UDP[3H]GlcNAc we identified [3H]GlcNAcPI as the first step of GPI biosynthesis. Both, alkylacylglycerol (major) and diacylglycerol are constituents of the lipid. Although inositolphosphoceramide is the main inositolphospholipid in epimastigotes, it does not incorporate GlcNAc. The de-N-acetylation step afforded [3H]GlcN(alkylacylglycerol)PI and we also detected the [3H]GlcN(lysoacyl)PI. A new metabolite, phosphoGlcN(lysoacyl)PI, which was formed on long incorporation times, was characterized by chemical and enzymatic degradations. Several [3H]-Man labelled GPI precursors were obtained by in vitro GDP[3H]-Man labelling in the presence of UDPGlcNAc. All of them were sensitive to PI-PLC and to saponification conditions, thus, supporting a glycerolipid structure. © 2003 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi
Autor:Bertello, L.E.; Alves, M.J.M.; Colli, W.; De Lederkremer, R.M.
Filiación:CIHIDECAR, Depto. de Quím. Orgán., Ciudad Universitaria, 1428 Buenos Aires, Argentina
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, C.P. 26077, 05599-970 São Paulo, Brazil
Palabras clave:Ceramide; Glycoinositolphospholipids; GPI anchors; Inositolphosphoceramide; T. cruzi; ceramide derivative; inositol derivative; inositol phosphoceramide; unclassified drug; acetylation; article; biosynthesis; chemical structure; controlled study; enzyme degradation; epimastigote; nonhuman; priority journal; saponification; Trypanosoma cruzi; Mammalia; Trypanosoma; Trypanosoma cruzi
Año:2004
Volumen:133
Número:1
Página de inicio:71
Página de fin:80
DOI: http://dx.doi.org/10.1016/j.molbiopara.2003.09.007
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v133_n1_p71_Bertello

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Citas:

---------- APA ----------
Bertello, L.E., Alves, M.J.M., Colli, W. & De Lederkremer, R.M. (2004) . Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi. Molecular and Biochemical Parasitology, 133(1), 71-80.
http://dx.doi.org/10.1016/j.molbiopara.2003.09.007
---------- CHICAGO ----------
Bertello, L.E., Alves, M.J.M., Colli, W., De Lederkremer, R.M. "Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi" . Molecular and Biochemical Parasitology 133, no. 1 (2004) : 71-80.
http://dx.doi.org/10.1016/j.molbiopara.2003.09.007
---------- MLA ----------
Bertello, L.E., Alves, M.J.M., Colli, W., De Lederkremer, R.M. "Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 133, no. 1, 2004, pp. 71-80.
http://dx.doi.org/10.1016/j.molbiopara.2003.09.007
---------- VANCOUVER ----------
Bertello, L.E., Alves, M.J.M., Colli, W., De Lederkremer, R.M. Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi. Mol. Biochem. Parasitol. 2004;133(1):71-80.
http://dx.doi.org/10.1016/j.molbiopara.2003.09.007