Artículo

Espinosa, J.M.; Portal, D.; Lobo, G.S.; Pereira, C.A.; Alonso, G.D.; Gómez, E.B.; Lan, G.H.; Pomar, R.V.R.; Flawiá, M.M.; Torres, H.N. "Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein" (2003) Molecular and Biochemical Parasitology. 131(1):35-44
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Abstract:

A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)2Cys(X)4His(X)4Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of about 42 and 63kDa. Western blot analysis of cell extracts using an anti-PZFP1 antibody recognized a major band of 41kDa. Electrophoretic mobility shift analysis demonstrated that both, recombinant and native PZFP1, specifically interact with single-stranded DNA or RNA oligonucleotides carrying recognition sequences of other CCHC proteins. The protein was localized mainly in the cytoplasm and nucleus as observed by indirect immunofluorescence analysis. PZFP1 interacted specifically with a T. cruzi serine-arginine-rich protein (TcSR) in a yeast two-hybrid assay, suggesting a role in pre-mRNA processing. © 2003 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein
Autor:Espinosa, J.M.; Portal, D.; Lobo, G.S.; Pereira, C.A.; Alonso, G.D.; Gómez, E.B.; Lan, G.H.; Pomar, R.V.R.; Flawiá, M.M.; Torres, H.N.
Filiación:Fac. de Ciencias Exactas y Naturales, Inst. Invest. Ing. Genet. Biol. M., Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Molecular Biology, Max Planck Inst. Biophysical Chem., Am Fassberg 11, D-37077 Göttingen, Germany
Palabras clave:CCHC-type zinc finger; Poly-zinc finger protein; Serine-arginine-rich protein; TcPZFP1; TcSR; Trypanosoma cruzi; arginine; cell extract; double stranded DNA; oligonucleotide; protein; protein antibody; protozoal protein; recombinant protein; serine; zinc finger protein; amino acid sequence; article; cell nucleus; cellular distribution; cytoplasm; Escherichia coli; gel mobility shift assay; immunofluorescence; molecular recognition; molecular weight; nonhuman; nucleotide sequence; priority journal; protein analysis; protein DNA interaction; protein induction; protein localization; protein motif; protein protein interaction; protein purification; protein RNA binding; Trypanosoma cruzi; two hybrid system; Western blotting; yeast; Trypanosoma; Trypanosoma cruzi
Año:2003
Volumen:131
Número:1
Página de inicio:35
Página de fin:44
DOI: http://dx.doi.org/10.1016/S0166-6851(03)00187-7
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; protein, 67254-75-5; serine, 56-45-1, 6898-95-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v131_n1_p35_Espinosa

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Citas:

---------- APA ----------
Espinosa, J.M., Portal, D., Lobo, G.S., Pereira, C.A., Alonso, G.D., Gómez, E.B., Lan, G.H.,..., Torres, H.N. (2003) . Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein. Molecular and Biochemical Parasitology, 131(1), 35-44.
http://dx.doi.org/10.1016/S0166-6851(03)00187-7
---------- CHICAGO ----------
Espinosa, J.M., Portal, D., Lobo, G.S., Pereira, C.A., Alonso, G.D., Gómez, E.B., et al. "Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein" . Molecular and Biochemical Parasitology 131, no. 1 (2003) : 35-44.
http://dx.doi.org/10.1016/S0166-6851(03)00187-7
---------- MLA ----------
Espinosa, J.M., Portal, D., Lobo, G.S., Pereira, C.A., Alonso, G.D., Gómez, E.B., et al. "Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein" . Molecular and Biochemical Parasitology, vol. 131, no. 1, 2003, pp. 35-44.
http://dx.doi.org/10.1016/S0166-6851(03)00187-7
---------- VANCOUVER ----------
Espinosa, J.M., Portal, D., Lobo, G.S., Pereira, C.A., Alonso, G.D., Gómez, E.B., et al. Trypanosoma cruzi poly-zinc finger protein: A novel DNA/RNA-binding CCHC-zinc finger protein. Mol. Biochem. Parasitol. 2003;131(1):35-44.
http://dx.doi.org/10.1016/S0166-6851(03)00187-7