Artículo

Portal, D.; Lobo, G.S.; Kadener, S.; Prasad, J.; Espinosa, J.M.; Pereira, C.A.; Tang, Z.; Lin, R.-J.; Manley, J.L.; Kornblihtt, A.R.; Flawiá, M.M.; Torres, H.N. "Trypanosoma cruzi TcSRPK, the first protozoan member of the SRPK family, is biochemically and functionally conserved with metazoan SR protein-specific kinases" (2003) Molecular and Biochemical Parasitology. 127(1):9-21
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Abstract:

A novel SR protein-specific kinase (SRPK) from the SRPK family was identified for the first time in a protozoan organism. The primary structure of the protein, named TcSRPK, presents a significant degree of identity with other metazoan members of the family. In vitro phosphorylation experiments showed that TcSRPK has the same substrate specificity relative to other SRPKs. TcSRPK was able to generate a mAb104-recognized phosphoepitope, a SRPK landmark. Expression of TcSRPK in different Schizosaccharomyces pombe strains lead to conserved phenotypes, indicating that TcSRPK is a functional homologue of metazoan SRPKs. In functional alternative splicing assays in vivo in HeLa cells, TcSRPK enhanced SR protein-dependent inclusion of the EDI exon of the fibronectin minigene. When tested in vitro, it inhibited splicing either on nuclear extracts or on splicing-deficient S100 extracts complemented with ASF/SF2. This inhibition was similar to that observed with human SRPK1. This work constitutes the first report of a member of this family of proteins and the existence of an SR-network in a protozoan organism. The implications in the origins and control of splicing are discussed. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi TcSRPK, the first protozoan member of the SRPK family, is biochemically and functionally conserved with metazoan SR protein-specific kinases
Autor:Portal, D.; Lobo, G.S.; Kadener, S.; Prasad, J.; Espinosa, J.M.; Pereira, C.A.; Tang, Z.; Lin, R.-J.; Manley, J.L.; Kornblihtt, A.R.; Flawiá, M.M.; Torres, H.N.
Filiación:Fac. de Ciencias Exactas y Naturales, Inst. Invest. Ingenieria Genet. B., Universidad de Buenos Aires, Buenos Aires, Argentina
Lab. de Fisiol. y Biol. Molecular, Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Department of Biological Sciences, Columbia University, New York, NY 10027, United States
Division of Molecular Biology, Beckman Res. Inst. the City of Hope, Duarte, CA, United States
Palabras clave:SR protein kinase; SR-network; SRPK; Trans-splicing; Trypanosoma cruzi; cell extract; epitope; fibronectin; isoenzyme; monoclonal antibody; monoclonal antibody 104; protein kinase; protein S 100; protozoal protein; ribonucleoprotein; RNA; serine arginine protein; serine arginine protein kinase; splicing factor 2; unclassified drug; alternative RNA splicing; article; cell nucleus; controlled study; enzyme phosphorylation; enzyme specificity; exon; fungal strain; gene; HeLa cell; human; human cell; in vitro study; in vivo study; inhibition kinetics; metazoon; molecular recognition; nonhuman; nucleotide sequence; phenotype; priority journal; protein expression; protein family; protein function; protein structure; Schizosaccharomyces pombe; sequence homology; Trypanosoma cruzi; Amino Acid Sequence; Animals; Antibodies, Monoclonal; Cloning, Molecular; Genes, Protozoan; Globins; Hela Cells; Humans; Molecular Sequence Data; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases; Schizosaccharomyces; Sequence Alignment; Sequence Homology, Amino Acid; Species Specificity; Trypanosoma cruzi; Metazoa; Protozoa; Schizosaccharomyces; Schizosaccharomyces pombe; Trypanosoma; Trypanosoma cruzi
Año:2003
Volumen:127
Número:1
Página de inicio:9
Página de fin:21
DOI: http://dx.doi.org/10.1016/S0166-6851(02)00299-2
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:fibronectin, 86088-83-7; protein kinase, 9026-43-1; RNA, 63231-63-0; Antibodies, Monoclonal; Globins, 9004-22-2; Protein-Serine-Threonine Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v127_n1_p9_Portal

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Citas:

---------- APA ----------
Portal, D., Lobo, G.S., Kadener, S., Prasad, J., Espinosa, J.M., Pereira, C.A., Tang, Z.,..., Torres, H.N. (2003) . Trypanosoma cruzi TcSRPK, the first protozoan member of the SRPK family, is biochemically and functionally conserved with metazoan SR protein-specific kinases. Molecular and Biochemical Parasitology, 127(1), 9-21.
http://dx.doi.org/10.1016/S0166-6851(02)00299-2
---------- CHICAGO ----------
Portal, D., Lobo, G.S., Kadener, S., Prasad, J., Espinosa, J.M., Pereira, C.A., et al. "Trypanosoma cruzi TcSRPK, the first protozoan member of the SRPK family, is biochemically and functionally conserved with metazoan SR protein-specific kinases" . Molecular and Biochemical Parasitology 127, no. 1 (2003) : 9-21.
http://dx.doi.org/10.1016/S0166-6851(02)00299-2
---------- MLA ----------
Portal, D., Lobo, G.S., Kadener, S., Prasad, J., Espinosa, J.M., Pereira, C.A., et al. "Trypanosoma cruzi TcSRPK, the first protozoan member of the SRPK family, is biochemically and functionally conserved with metazoan SR protein-specific kinases" . Molecular and Biochemical Parasitology, vol. 127, no. 1, 2003, pp. 9-21.
http://dx.doi.org/10.1016/S0166-6851(02)00299-2
---------- VANCOUVER ----------
Portal, D., Lobo, G.S., Kadener, S., Prasad, J., Espinosa, J.M., Pereira, C.A., et al. Trypanosoma cruzi TcSRPK, the first protozoan member of the SRPK family, is biochemically and functionally conserved with metazoan SR protein-specific kinases. Mol. Biochem. Parasitol. 2003;127(1):9-21.
http://dx.doi.org/10.1016/S0166-6851(02)00299-2