Artículo

Portal, D.; Espinosa, J.M.; Lobo, G.S.; Kadener, S.; Pereira, C.A.; De La Mata, M.; Tang, Z.; Lin, R.-J.; Kornblihtt, A.R.; Baralle, F.E.; Flawiá, M.M.; Torres, H.N. "An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing" (2003) Molecular and Biochemical Parasitology. 127(1):37-46
La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

A novel serine-arginine-rich protein designated TcSR was identified in Trypanosoma cruzi. The deduced amino acid sequence reveals that TcSR is a member of the SR protein family of splicing factors that contains two RNA-binding domains at the N-terminal side and several serine-arginine repeats at the COOH-terminus. Over expression of either TcSR or the human SR-protein associated splicing factor/splicing factor 2 (ASF/SF2) in wild-type Schizosaccharomyces pombe, provoked an elongated phenotype similar to that of fission yeast over expressing the SR-containing splicing factor Prp2, a U2AF65 orthologue. When a double mutant strain lacking two SR protein-specific protein kinases was used, expression of TcSR or human SR ASF/SF2 splicing factor reverted the mutant to a wild-type phenotype. Transient expression of TcSR in HeLa cells stimulated the inclusion of the EDI exon of human fibronectin in an in vivo functional alternative cis-splicing assay. Inclusion was dependent on a splicing enhancer sequence present in the EDI exon. In addition, TcSR and peptides carrying TcSR-RS domain sequences were phosphorylated by a human SR protein kinase. These results indicate that TcSR is a member of the SR splicing network and that some components common to the trans- and cis-splicing machineries evolved from the early origins of the eukaryotic lineage. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing
Autor:Portal, D.; Espinosa, J.M.; Lobo, G.S.; Kadener, S.; Pereira, C.A.; De La Mata, M.; Tang, Z.; Lin, R.-J.; Kornblihtt, A.R.; Baralle, F.E.; Flawiá, M.M.; Torres, H.N.
Filiación:Fac. de Ciencias Exactas y Naturales, Inst. Invest. Ing. Genet. Biol. M., Universidad de Buenos Aires, Buenos Aires, Argentina
Lab. de Fisiol. y Biol. Molecular, Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Department of Molecular Biology, Beckman Res. Inst. the City of Hope, Duarte, CA, United States
Intl. Ctr. for Genetic Eng./Biotech., Trieste, Italy
Palabras clave:cis-Splicing; Evolution; SR protein; trans-Splicing; Trypanosoma cruzi; arginine; fibronectin; isoprotein; peptide; protein kinase; protein Prp2; protozoal protein; ribonucleoprotein; serine; serine arginine protein; serine arginine protein kinase; splicing factor 2; unclassified drug; amino acid sequence; amino terminal sequence; article; assay; controlled study; enhancer region; eukaryote evolution; exon; fungus mutant; gene overexpression; HeLa cell; human; human cell; in vivo study; molecular evolution; nonhuman; nucleotide sequence; phenotype; priority journal; protein family; protein phosphorylation; RNA splicing; Schizosaccharomyces pombe; trans splicing; Trypanosoma cruzi; wild type; yeast; Amino Acid Sequence; Animals; Arginine; Evolution, Molecular; Hela Cells; Humans; Protozoan Proteins; RNA Splicing; Schizosaccharomyces; Sequence Alignment; Serine; Trypanosoma cruzi; Eukaryota; Schizosaccharomyces; Schizosaccharomyces pombe; Trypanosoma; Trypanosoma cruzi
Año:2003
Volumen:127
Número:1
Página de inicio:37
Página de fin:46
DOI: http://dx.doi.org/10.1016/S0166-6851(02)00301-8
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; fibronectin, 86088-83-7; protein kinase, 9026-43-1; serine, 56-45-1, 6898-95-9; Arginine, 74-79-3; Protozoan Proteins; Serine, 56-45-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v127_n1_p37_Portal

Referencias:

  • (1988) Weekly Epidemiological Record, 1-2, pp. 1-4
  • Brenner, Z., Biology of Trypanosoma cruzi (1973) Annu. Rev. Microbiol., 27, pp. 347-382
  • Vanhamme, L., Pays, E., Control of gene expression in trypanosomes (1995) Microbiol. Rev., 59, pp. 223-240
  • Myler, P.J., Audleman, L., DeVos, T., Hixson, G., Kiser, P., Lemley, C., Leishmania major Friedlin chromosome 1 has an unusual distribution of protein-coding genes (1999) Proc. Natl. Acad. Sci. U.S.A., 96, pp. 2902-2906
  • Teixeira, S.M., Kirchhoff, L.V., Donelson, J.E., Post-transcriptional elements regulating expression of mRNAs from amastin/tuzin gene cluster in Trypanosoma cruzi (1995) J. Biol. Chem., 270, pp. 22586-22594
  • Hotz, H.R., Hartmann, C., Huober, K., Hug, M., Clayton, C., Mechanisms of developmental regulation in Trypanosoma brucei: A polypyrimidine tract in the 3′ untranslated region of a surface protein mRNA affects RNA abundance and translation (1997) Nucleic Acids Res., 25, pp. 3017-3025
  • Nozaki, T., Cross, G.A., Effects of 3′ untranslated and intergenic regions on gene expression in Trypanosoma cruzi (1995) Mol. Biochem. Parasitol., 75, pp. 55-67
  • Agabian, N., Trans-splicing of nuclear pre-mRNAs (1990) Cell, 61, pp. 1157-1160
  • Nilsen, T.W., Evolutionary origin of SL-addition trans-splicing: Still an enigma (2001) Trends Genet., 17, pp. 678-680
  • Vandenberghe, A.E., Meedel, T.H., Hastings, K.E.M., MRNA 5′-leader trans-splicing in the chordates (2001) Genes Dev., 15, pp. 294-303
  • Stover, N.A., Steele, R.E., Trans-spliced leader addition to mRNAs in a cnidarian (2001) Proc. Natl. Acad. Sci. U.S.A., 98, pp. 5693-5698
  • Nilsen, T.W., Trans-splicing of nematode pre-messenger RNA (1993) Trends Genet., 17, pp. 678-680
  • Mair, G., Shi, H., Li, H., Djikeng, A., Aviles, H.O., Bishop, J.R., A new twist in trypanosome RNA metabolism: Cis-splicing of pre-mRNA (2000) RNA, 6, pp. 163-169
  • Gunzl, A., Cross, M., Bindereif, A., Domain structure of U2 and U4/U6 small nuclear ribonucleoprotein particles from Trypanosoma brucei: Identification of trans-spliceosomal specific RNA-protein interactions (1992) Mol. Cell Biol., 12, pp. 468-479
  • Tschudi, C., Ullu, E., Destruction of U2, U4, or U6 small nuclear RNA blocks trans splicing in trypanosome cells (1990) Cell, 61, pp. 459-466
  • Palfi, Z., Lane, W.S., Bindereif, A., Biochemical and functional characterization of the cis-spliceosomal U1 small nuclear RNP from Trypanosoma brucei (2002) Mol. Biochem. Parasitol., 121, pp. 233-243
  • Bruzik, J.P., Maniatis, T., Spliced leader RNAs from lower eukaryotes are trans-spliced in mammalian cells (1992) Nature, 360, pp. 692-695
  • Krämer, A., The structure and function of proteins involved in mammalian pre-mRNA splicing (1996) Annu. Rev. Biochem., 65, pp. 367-409
  • Fu, X.D., The superfamily of arginine/serine-rich splicing factors (1995) RNA, 1, pp. 663-680
  • Manley, J.L., Tacke, R., SR proteins and splicing control (1996) Genes Dev., 9, pp. 284-293
  • Valcárcel, J., Green, M.R., The SR protein family: Pleiotropic functions in pre-mRNA splicing (1996) Trends Biochem. Sci., 21, pp. 296-301
  • Hastings, M.L., Krainer, A.R., Splicing in the new millennium (2001) Curr. Opin. Cell Biol., 13, pp. 302-308
  • Fu, X.D., Specific commitment of different pre-RNAs to splicing by single SR proteins (1993) Nature, 365, pp. 82-85
  • Jamison, S.F., Pasman, Z., Wang, J., Will, C., Lührmann, R., Manley, J.L., U1 snRNP-ASF/SF2 interaction and 5′ splice-site recognition: Characterization of requirements (1995) Nucleic Acids Res., 23, pp. 3260-3267
  • Cáceres, J.F., Stamm, S., Helfman, D.M., Krainer, A.R., Regulation of alternative splicing in vivo by overexpression of antagonistic pre-mRNAs spicing factors (1994) Science, 265, pp. 1706-1709
  • Wang, J., Manley, J.L., Overexpression of SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways (1995) RNA, 1, pp. 335-346
  • Mardon, H.J., Sebastio, G., Baralle, F.E., A role for exon sequences in alternative splicing of the human fibronectin gene (1987) Nucleic Acids Res., 15, pp. 7725-7733
  • Laviguer, A., LaBranche, H., Kornblihtt, A.R., Chabot, B., A splicing enhancer in the human fibronectin alternative EDI exon interacts with SR proteins and stimulates U2 snRNP binding (1993) Genes Dev., 7, pp. 2405-2417
  • Tian, M., Maniatis TA splicing enhancer complex controls alternative splicing of double-sex pre-mRNA (1993) Cell, 74, pp. 105-114
  • Tacke, R., Manley, J.L., The human splicing factors ASF/SF2 and SC35 possess distinct, functional significant RNA binding specificities (1995) EMBO J., 14, pp. 3540-3551
  • Muro, A.F., Caputi, M., Pariyarath, R., Pagani, F., Buratti, E., Baralle, F.E., Regulation of fibronectin EDA exon alternative splicing: Possible role of RNA secondary structure (1999) Mol. Cell Biol., 19, pp. 2657-2671
  • Ismaili, N., Pérez-Morga, D., Walsh, P., Mayeda, A., Pays, A., Tebabi, P., Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins (1999) Mol. Biochem. Parasitol., 102, pp. 103-115
  • Espinosa, J., Martinetto, H., Portal, D., D'Angelo, M., Torres, H.N., Flawiá, M.M., Trypanosoma cruzi factors interacting with AP-1 sequences (1999) J. Eukar. Microbiol., 46, pp. 516-521
  • Voojtek, A.B., Cooper, J.A., Hollenberg, S.M., (1997) The Yeast Two-Hybrid System, pp. 29-42. , Bartel PL, Fields S, editors. New York, NY: Oxford University Press
  • Gómez, E.B., Santori, M.I., Laria, S., Engel, J.C., Swindle, J., Eisen, H., Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins (2001) Mol. Biochem. Parasitol., 113, pp. 97-108
  • Paabo, S., Higuchi, R.G., Wilson, A.C., Ancient DNA and the polymerase chain reaction (1989) J. Biol. Chem., 264, pp. 9709-9712
  • Moreno, S., Klar, S., Nurse, P., Molecular genetics analysis of fission yeast Schizosaccharomyces pombe (1991) Methods Enzymol., 194, pp. 795-823
  • Maundrell, K., Thiamine-repressible expression vectors pREP and pRIP for fission yeast (1993) Gene, 123, pp. 127-130
  • Tang, Z., Kuo, T., Shen, J., Lin, R.-J., Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1 (2000) Mol. Cell Biol., 20, pp. 816-824
  • Caputi, M., Casari, G., Guenzi, S., Tagliabue, R., Sidoli, A., Melo, C.A., A novel bipartite splicing enhancer modulates the differential processing of human fibronectin EDA exon (1994) Nucleic Acids Res., 22, pp. 1018-1022
  • Cramer, P., Cáceres, J.F., Cazalla, D., Kadener, S., Muro, A.F., Baralle, F.E., Coupling of transcription with alternative splicing: RNA Pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer (1999) Mol. Cell, 4, pp. 251-258
  • Papoutsopoulou, S., Nikolakaki, E., Giannakouros, T., SRPK1 and LBR protein kinases show identical substrate specificities (1999) Biochem. Biophys. Res. Commun., 255, pp. 602-607
  • Birney, E., Kumar, S., Krainer, A.R., Analysis of the RNA-recognition motif and RS and RGG domains: Conservation in metazoan pre-mRNA splicing factors (1993) Nucleic Acids Res., 21, pp. 5803-5816
  • Zuo, P., Manley, J.L., Functional domains of the human splicing factor ASF/SF2 (1993) EMBO J., 12, pp. 4727-4737
  • Cáceres, J.F., Krainer, A.R., Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains. Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains (1993) EMBO J., 12, pp. 4715-4726
  • Xiao, S.H., Manley, J.L., Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2 (1998) EMBO J., 17, pp. 6359-6367
  • Wang, J., Xiao, S.H., Manley, J.L., Genetic analysis of the SR protein ASF/SF2: Interchangeability of RS domains and negative control of splicing (1998) Genes Dev., 12, pp. 2222-2233
  • Potashkin, J., Kim, D., Fons, M., Humphrey, T., Frendewey, D., Cell-division-cycle dependent defects associated with fission yeast pre-mRNA splicing mutants (1998) Curr. Genet., 34, pp. 153-163
  • Zhu, J., Krainer, A.R., Pre-mRNA splicing in the absence of a SR protein SR domain (2000) Genes Dev., 14, pp. 3166-3178
  • Kornblihtt, A.R., Vibe-Pedersen, K., Baralle, F.E., Human fibronectin: Molecular cloning evidence for two mRNA species differing by and internal segment coding for a structural domain (1984) EMBO J., 3, pp. 221-226
  • Vibe-Pedersen, K., Kornblihtt, A.R., Baralle, F.E., Expression of a human alpha-globin/fibronectin gene hybrid generates two mRNAs by alternative splicing (1984) EMBO J., 3, pp. 2511-2516
  • Gui, J.F., Lane, W.S., Fu, X.D., A serine kinase regulates intracellular localization of splicing factors in the cell cycle (1994) Nature, 369, pp. 678-682
  • Flink, I.L., Morkin, E., Alternatively processed isoforms of cellular nucleic acid-binding protein interact with a suppressor region of the human beta-myosin heavy chain gene (1995) J. Biol. Chem., 270, pp. 6959-6965
  • Pellizzoni, L., Lotti, F., Maras, B., Pierandrei-Amaldi, P., Cellular nucleic acid binding protein binds a conserved region of the 5′ UTR of Xenopus laevis ribosomal protein mRNAs (1997) J. Mol. Biol., 267, pp. 264-275
  • Taylor, F.M., Mertindale, D.W., Retroviral-type zinc fingers and glycine-rich repeats in a protein encoded by cnjB, a Tetrahymena gene active during meiosis (1993) Nucleic Acids Res., 21, pp. 4610-4614
  • Frank, D., Guthrie, C., An essential splicing factor, SLU7, mediates 3 splice site choice in yeast (1992) Genes Dev., 6, pp. 2112-2124
  • Cavaloc, Y., Popielarz, M., Fuchs, J.P., Gattoni, R., Stévenin, J., Characterization and cloning of the human splicing factor 9G8: A novel 35 kDa factor of the serine/arginine protein family (1994) EMBO J., 13, pp. 2639-2649
  • Ismaili, N., Perez-Morga, D., Walsh, P., Cadogan, M., Pays, A., Tebabi, P., Characterization of a Trypanosoma brucei SR domain-containing protein bearing homology to cis-spliceosomal U1 70 kDa proteins (2000) Mol. Biochem. Parasitol., 106, pp. 109-120

Citas:

---------- APA ----------
Portal, D., Espinosa, J.M., Lobo, G.S., Kadener, S., Pereira, C.A., De La Mata, M., Tang, Z.,..., Torres, H.N. (2003) . An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing. Molecular and Biochemical Parasitology, 127(1), 37-46.
http://dx.doi.org/10.1016/S0166-6851(02)00301-8
---------- CHICAGO ----------
Portal, D., Espinosa, J.M., Lobo, G.S., Kadener, S., Pereira, C.A., De La Mata, M., et al. "An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing" . Molecular and Biochemical Parasitology 127, no. 1 (2003) : 37-46.
http://dx.doi.org/10.1016/S0166-6851(02)00301-8
---------- MLA ----------
Portal, D., Espinosa, J.M., Lobo, G.S., Kadener, S., Pereira, C.A., De La Mata, M., et al. "An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing" . Molecular and Biochemical Parasitology, vol. 127, no. 1, 2003, pp. 37-46.
http://dx.doi.org/10.1016/S0166-6851(02)00301-8
---------- VANCOUVER ----------
Portal, D., Espinosa, J.M., Lobo, G.S., Kadener, S., Pereira, C.A., De La Mata, M., et al. An early ancestor in the evolution of splicing: A Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing. Mol. Biochem. Parasitol. 2003;127(1):37-46.
http://dx.doi.org/10.1016/S0166-6851(02)00301-8