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Santori, M.I.; Laría, S.; Gómez, E.B.; Espinosa, I.; Galanti, N.; Téllez-Iñón, M.T. "Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi" (2002) Molecular and Biochemical Parasitology. 121(2):225-232
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Abstract:

Trypanosoma cruzi CRK3 gene encodes a Cdc2p related protein kinase (CRK). To establish if it has a role in the regulation of the parasite cell cycle we studied CRK3 expression and activity throughout three life cycle stages. CRK3 from epimastigote soluble extracts interacted with p13suc1-beads. Endogenous CRK3 phosphorylated histone H1 and this activity was inhibited by specific CDK inhibitors: Olomoucine, Flavopiridol and Roscovitine. Flavopiridol partially inhibited the growth of T. cruzi epimastigotes at 50 nM, the lowest concentration used, but even with the highest (5 μM), cell growth was not completely arrested. CRK3 from Flavopiridol-inhibited epimastigote extracts exhibited a dose dependent inhibition of histone H1 phosphorylation. T. cruzi p13suc1-binding CRK displayed the same inhibition profile. This suggests that CRK3 is the enzyme responsible for the majority of the kinase activity associated with p13suc1. CRK3 activity of hydroxyurea (HU) synchronized epimastigotes peaked in G2/M boundary while the kinase activity associated to p13suc1-beads increased at the same time point but remained high until late G2/M. In addition, CRK3 expression was constant during the cell cycle. This is a common pattern of CDK activity regulation. Taken together, these results support the idea that CRK3 is involved in control of the cell cycle in T. cruzi. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi
Autor:Santori, M.I.; Laría, S.; Gómez, E.B.; Espinosa, I.; Galanti, N.; Téllez-Iñón, M.T.
Filiación:Instituto De Investigaciones En Ingeniería Genética Y Biología Molecular, Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires (UBA), Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Programa De Desarrollo De Biología Molecular Y Celular, Instituto De Ciencias Biomédicas (ICBM), Universidad De Chile, Santiago, Chile
Palabras clave:Cell cycle control; Cyclin-dependent kinase; Flavopiridol; Hydroxyurea synchronization; Trypanosoma cruzi; flavopiridol; histone H1; hydroxyurea; olomoucine; protein; protein kinase; protein kinase inhibitor; protein p13; roscovitine; unclassified drug; article; cell cycle; cell cycle G2 phase; cell division; cell synchronization; concentration response; developmental stage; drug effect; enzyme activity; enzyme binding; epimastigote; gene expression; growth inhibition; mitosis inhibition; nonhuman; parasite development; phosphorylation; priority journal; protein interaction; regulatory mechanism; Trypanosoma cruzi; Animals; CDC2 Protein Kinase; Cell Cycle; Cyclin-Dependent Kinases; Enzyme Inhibitors; Flavonoids; Gene Expression Regulation; Hydroxyurea; Piperidines; Protozoan Proteins; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi
Año:2002
Volumen:121
Número:2
Página de inicio:225
Página de fin:232
DOI: http://dx.doi.org/10.1016/S0166-6851(02)00039-7
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:CDC2 Protein Kinase, EC 2.7.1.37; CRK3 kinase, protozoa, EC 2.7.10.-; Cyclin-Dependent Kinases, EC 2.7.1.37; Enzyme Inhibitors; Flavonoids; flavopiridol, 146426-40-6; Hydroxyurea, 127-07-1; Piperidines; Protozoan Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v121_n2_p225_Santori

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Citas:

---------- APA ----------
Santori, M.I., Laría, S., Gómez, E.B., Espinosa, I., Galanti, N. & Téllez-Iñón, M.T. (2002) . Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi. Molecular and Biochemical Parasitology, 121(2), 225-232.
http://dx.doi.org/10.1016/S0166-6851(02)00039-7
---------- CHICAGO ----------
Santori, M.I., Laría, S., Gómez, E.B., Espinosa, I., Galanti, N., Téllez-Iñón, M.T. "Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi" . Molecular and Biochemical Parasitology 121, no. 2 (2002) : 225-232.
http://dx.doi.org/10.1016/S0166-6851(02)00039-7
---------- MLA ----------
Santori, M.I., Laría, S., Gómez, E.B., Espinosa, I., Galanti, N., Téllez-Iñón, M.T. "Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi" . Molecular and Biochemical Parasitology, vol. 121, no. 2, 2002, pp. 225-232.
http://dx.doi.org/10.1016/S0166-6851(02)00039-7
---------- VANCOUVER ----------
Santori, M.I., Laría, S., Gómez, E.B., Espinosa, I., Galanti, N., Téllez-Iñón, M.T. Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi. Mol. Biochem. Parasitol. 2002;121(2):225-232.
http://dx.doi.org/10.1016/S0166-6851(02)00039-7