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Abstract:

In this study, the effects of sublethal concentrations of the carbamate carbaryl on the cholinesterase (ChE) and carboxylesterase (CES) activities present in the oligochaete Lumbriculus variegatus and in the pigmented Biomphalaria glabrata gastropod were investigated. The results showed that ChE activity from both species was inhibited by in vivo and in vitro exposure to carbaryl, with EC50 and IC50 values approximately 20 times lower for the oligochaete than for the gastropod. On the other hand, the recovery process in uncontaminated media was more efficient in oligochaetes than in snails. Thus, in only 2 h the oligochaetes showed no inhibition with respect to control values whereas the snails did not reach control values even after 48 h of being in pesticide-free water. CES activity was investigated in whole body soft tissue homogenates using three different substrates: p-nitrophenyl butyrate, 1-naphthyl acetate (NA) and 2-NA. In addition, the presence of multiple CES isozymes in L. variegatus and B. glabrata extracts, with activity towards 1- and 2-NA, was confirmed by native polyacrylamide electrophoresis. In both species, the activities measured using the naphthyl substrates were higher than the activity towards p-nitrophenyl butyrate. In addition, B. glabrata showed a higher CES activity than L. variegatus independently of the substrate used. In L. variegatus, in vivo CES activity towards the different substrates was less sensitive to carbaryl inhibition than ChE activity. In contrast, in B. glabrata, CES activity towards p-nitrophenyl butyrate was inhibited at lower insecticide concentrations than ChE. The results of this study contribute to the knowledge of the sensitivity of non-target freshwater invertebrate Type B-esterases towards pesticides. © 2009 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl
Autor:Kristoff, G.; Guerrero, N.R.V.; Cochón, A.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Nuñez, 1428, Buenos Aires, Argentina
Palabras clave:Biomphalaria glabrata; Carbaryl; Carboxylesterases; Cholinesterases; Lumbriculus variegatus; 1 naphthyl acetate; 2 naphthyl acetate; butyric acid 4 nitrophenyl ester; butyric acid derivative; carbaril; carboxylesterase; cholinesterase; naphthalene derivative; unclassified drug; annelid; carbamate; concentration (composition); electrokinesis; enzyme; gastropod; inhibition; pesticide; sensitivity analysis; sublethal effect; animal tissue; annelid worm; article; Biomphalaria glabrata; concentration response; controlled study; ecotoxicity; enzyme activity; enzyme assay; enzyme inhibition; enzyme substrate complex; IC 50; in vitro study; in vivo study; lethal dose; Lumbriculus variegatus; nonhuman; polyacrylamide gel electrophoresis; priority journal; species comparison; species difference; tissue homogenate; toxicity testing; Animals; Biomphalaria; Carbaryl; Carboxylic Ester Hydrolases; Cholinesterases; Enzyme Inhibitors; Inhibitory Concentration 50; Oligochaeta; Pesticides; Time Factors; Water Pollutants, Chemical; Biomphalaria glabrata; Gastropoda; Invertebrata; Lumbriculus variegatus; Oligochaeta (Metazoa)
Año:2010
Volumen:96
Número:2
Página de inicio:115
Página de fin:123
DOI: http://dx.doi.org/10.1016/j.aquatox.2009.10.001
Título revista:Aquatic Toxicology
Título revista abreviado:Aquat. Toxicol.
ISSN:0166445X
CODEN:AQTOD
CAS:1 naphthyl acetate, 830-81-9; carbaril, 63-25-2; carboxylesterase, 59536-71-9, 83380-83-0, 9016-18-6, 9028-01-7; cholinesterase, 9001-08-5; Carbaryl, 63-25-2; Carboxylic Ester Hydrolases, 3.1.1.-; Cholinesterases, 3.1.1.8; Enzyme Inhibitors; Pesticides; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v96_n2_p115_Kristoff

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Citas:

---------- APA ----------
Kristoff, G., Guerrero, N.R.V. & Cochón, A.C. (2010) . Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl. Aquatic Toxicology, 96(2), 115-123.
http://dx.doi.org/10.1016/j.aquatox.2009.10.001
---------- CHICAGO ----------
Kristoff, G., Guerrero, N.R.V., Cochón, A.C. "Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl" . Aquatic Toxicology 96, no. 2 (2010) : 115-123.
http://dx.doi.org/10.1016/j.aquatox.2009.10.001
---------- MLA ----------
Kristoff, G., Guerrero, N.R.V., Cochón, A.C. "Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl" . Aquatic Toxicology, vol. 96, no. 2, 2010, pp. 115-123.
http://dx.doi.org/10.1016/j.aquatox.2009.10.001
---------- VANCOUVER ----------
Kristoff, G., Guerrero, N.R.V., Cochón, A.C. Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl. Aquat. Toxicol. 2010;96(2):115-123.
http://dx.doi.org/10.1016/j.aquatox.2009.10.001