Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The effects of cadmium and copper on the hormonal control of ovarian growth were evaluated on the estuarine crab Chasmagnathus granulata, by means of both in vivo (14 days exposure) and in vitro (24 h) assays. For both kind of assays, heavy metal concentrations of 0 (control), 0.5 mg/L of cadmium or 0.1 mg/L of copper were used. No significant (P > 0.05) change of the gonadosomatic index was observed in the in vivo assays with intact females exposed to heavy metals, while eyestalk-ablated exposed females showed significantly (P < 0.05) lower gonadosomatic index values than their respective controls. This latter result led us to consider the possibility that the interfered with extra-eyestalk hormones. In this sense, no differences were noted between control and heavy metals-exposed groups after co-incubating ovary with thoracic ganglion (the source of the gonad stimulating hormone). However, when ovary was incubated with methyl farnesoate or 17-hydroxyprogesterone, 3H-leucine incorporation was significantly lower in the heavy metals-exposed groups than in the controls, indicating a possible interference of cadmium and copper with the transduction pathway of those hormones. On the other hand, ovaries co-incubated in vitro with eyestalk tissue and exposed to either heavy metal showed significantly higher 3H-leucine incorporation than did the controls, suggesting an inhibitory effect of both heavy metals on the secretion of the gonad inhibiting hormone from the eyestalk tissue. Interference by copper and cadmium with the transduction mechanisms of gonad inhibiting hormone at the ovarian level does not appear to be a viable hypothesis, because the addition of eyestalk extracts to the incubation medium reversed the effect caused by each heavy metal. The results from the in vitro assays were in accordance with those obtained with the intact crabs in vivo. © 2004 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Interference of cadmium and copper with the endocrine control of ovarian growth, in the estuarine crab Chasmagnathus granulata
Autor:Medesani, D.A.; López Greco, L.S.; Rodríguez, E.M.
Filiación:Animal Physiology Laboratory, Dept. of Biodiv. and Exp. Biology, Univ. of Buenos Aires Cd. Univ., C1428EHA Buenos Aires, Argentina
Palabras clave:Cadmium; Copper; Crabs; Hormones; Reproduction; cadmium; copper; follistatin; gonadotropin; heavy metal; hydroxyprogesterone; leucine; bioassay; cadmium; copper; endocrine system; heavy metal; hormone; animal experiment; article; concentration (parameters); controlled study; crab; endocrine gland; environmental exposure; estuary; female; hormonal regulation; hormone release; nonhuman; ovary; ovary development; priority journal; reproductive toxicity; signal transduction; 17-alpha-Hydroxyprogesterone; Animals; Argentina; Brachyura; Cadmium; Copper; Fatty Acids, Unsaturated; Female; Ganglia, Invertebrate; Leucine; Muscle, Skeletal; Ovary; Tritium; Chasmagnathus granulata; Decapoda (Crustacea)
Año:2004
Volumen:69
Número:2
Página de inicio:165
Página de fin:174
DOI: http://dx.doi.org/10.1016/j.aquatox.2004.05.003
Título revista:Aquatic Toxicology
Título revista abreviado:Aquatic Toxicol.
ISSN:0166445X
CODEN:AQTOD
CAS:cadmium, 22537-48-0, 7440-43-9; copper, 15158-11-9, 7440-50-8; follistatin, 117628-82-7; gonadotropin, 63231-54-9; hydroxyprogesterone, 68-96-2; leucine, 61-90-5, 7005-03-0; 17-alpha-Hydroxyprogesterone, 68-96-2; Cadmium, 7440-43-9; Copper, 7440-50-8; Fatty Acids, Unsaturated; Leucine, 61-90-5; methyl farnesoate, 10485-70-8; Tritium, 10028-17-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v69_n2_p165_Medesani

Referencias:

  • (1995) Standard Methods for the Examination of Water and Wastewater, 19th Ed., , American Public Health Association, Washington, DC
  • Bigi, R., Verrengia-Guerrero, N., Rodríguez, E.M., Kesten, E., Medesani, D.A., Acute lethal toxicity and bioaccumulation of cadmium in the estuarine crab Chasmagnathus granulata (Decapoda, Brachyura) (1996) Pollution Processes in Coastal Environments, pp. 292-295. , J. Marcovecchio (Ed.), 2-6 December 1996, Mar del Plata, Argentina
  • Charniaux-Cotton, H., Payen, G., Crustacean reproduction (1988) Endocrinology of Selected Invertebrate Types, pp. 279-303. , H. Laufer, R.C.G. Downer (Eds.), Alan R. Liss, New York
  • (1990) Estudio para la Evaluación de la Contaminación en El Río de la Plata, Informe de Avance, , Argentine Hydrographic Navy Service, Buenos Aires, Argentina
  • Cooke, I.M., Haylett, B.A., Weatherby, T.M., Electrically elicited neurosecretory and electrical responses of the isolated crab sinus gland in normal and reduced calcium salines (1977) J. Exp. Biol., 101, pp. 125-149
  • De Fur, P.L., Crane, M., Ingersoll, C., Tattersfield, L., (1999) Endocrine Disruption in Invertebrates: Endocinology, Testing and Assessment, , SETAC Technical Publications Series, SETAC Press, Pensacola, FL
  • De Kleijn, D.P.V., Van Herp, F., Involvement of the hyperglycemic neurohormone family in the control of reproduction in decapod crustaceans (1998) Invert. Reprod. Dev., 33, pp. 263-272
  • Depledge, M.H., Forbes, T.L., Forbes, V.E., Evaluation of cadmium, copper, zinc, and iron concentrations and tissue distributions in the benthic crab, Dorippe granulata (De Haan, 1841) from Tolo Harbour, Hong Kong (1993) Environ. Pollut., 81, pp. 15-19
  • Eastman-Reks, S., Fingerman, M., Effects of neuroendocrine tissue and cyclic AMP on ovarian growth in vivo and in vitro in the fiddler crab Uca pugilator (1984) Comput. Biochem. Physiol., 79, pp. 679-684
  • Fingerman, M., Nagabhushanam, R., Sarojini, R., Vertebrate-type hormones in crustaceans: Localization, identification and functional significance (1993) Zool. Sci., 10, pp. 13-29
  • Fingerman, M., Devi, M., Reddy, P.S., Katyayani, R., Impact of heavy metal exposure on the nervous system and endocrine-mediated processes in crustaceans (1996) Zool. Stud., 35, pp. 1-8
  • Fingerman, M., Roles of neurotransmitters in regulating reproductive hormone release and gonadal maturation in decapod crustaceans (1997) Invert. Reprod. Dev., 31, pp. 47-54
  • Fingerman, M., Jackson, N.C., Nagabhushanam, R., Hormonally-regulated functions in crustaceans as biomarkers of environmental pollution (1998) Comput. Biochem. Physiol., 120, pp. 343-350
  • Homola, E., Chang, E.S., Methyl farnesoate: Crustacean juvenile hormone in search of function (1997) Comput. Biochem. Physiol., 117, pp. 347-356
  • Hutchinson, T.H., Reproductive and developmental effects of endocrine disrupters in invertebrates: In vitro and in vivo approaches (2002) Toxicol. Lett., 131, pp. 75-81
  • Kendall, R., Dickerson, R., Giesy, J., Suk, W., (1998) Principles and Processes for Evaluating Endocrine Disruption in Wildlife, , SETAC Technical Publications Series, SETAC Press, Pensacola, FL
  • Keller, R., Crustacean neuropeptides: Structures, functions and comparative aspects (1992) Experientia, 48, pp. 439-448
  • Kulkarni, G.K., Glade, L., Fingerman, M., Oogenesis and effects of neuroendocrine tissues on in vitro synthesis of protein by the ovary of the red swamp crayfish Procambarus clarkii (Girard) (1991) J. Crust. Biol., 11, pp. 513-522
  • Laufer, H., Ahl, J.S.B., Sagi, A., The role of juvenile hormones in crustacean reproduction (1993) Am. Zool., 33, pp. 365-374
  • López Greco, L.S., Rodríguez, E.M., Annual reproduction and growth of adult crabs Chasmagnathus granulata (Brachyura, Grapsidae) (1999) Cahiers de Biologie Marine, 40, pp. 155-164
  • López Greco, L.S., Sánchez, V., Nicoloso, G.L., Medesani, D.A., Rodríguez, E.M., Toxicity of cadmium and copper on larval and juvenile stages of the estuarine crab Chasmagnathus granulata (Decapoda, Grapsidae) (2001) Arch. Environ. Contamin. Toxicol., 41, pp. 333-338
  • Olmstead, A.W., Leblanc, G.L., Low exposure concentration effects of methoprene on endocrine-regulated processes in the crustacean Daphnia magna (2001) Toxicol. Sci., 62, pp. 268-273
  • Otsu, T., Biohormonal control of sexual cycle in the freshwater crab, Potamon dehaani (1963) Embryologia, 8, pp. 1-20
  • Quackenbush, L.S., Lobster reproduction: A review (1994) Crustaceana, 67, pp. 82-94
  • Quinitio, E.T., Hara, A., Yamauchi, K., Nakao, S., Changes in the steroid hormone and vitellogenin levels during the gametogenic cycle of the giant tiger shrimp, Penaeus monodon (1994) Comput. Biochem. Physiol., 109, pp. 21-26
  • Reddy, P.S., Tuberty, S.R., Fingerman, M., Effects of cadmium and mercury on ovarian maturation in the red swamp crayfish, Procambarus clarkii (1997) Ecotoxicol. Environ. Saf., 37, pp. 62-65
  • Rodríguez, E.M., Monserrat, J.M., Amín, O.A., Chronic toxicity of ethyl parathion and isobutoxyethanol ester of 2,4-dichlorophenoxyacetic acid to estuarine juvenile and adult crabs (1992) Arch. Environ. Contamin. Toxicol., 22, pp. 140-145
  • Rodríguez, E.M., López Greco, L.S., Fingerman, M., Inhibition of ovarian growth by cadmium, in the fiddler crab Uca pugilator (Decapoda, Ocypodidae) (2000) Ecotoxicol. Environ. Saf., 46, pp. 202-206
  • Rodríguez, E.M., López Greco, L.S., Medesani, D.A., Laufer, H., Fingerman, M., Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis (2002) Gen. Comp. Endocrinol., 125, pp. 34-40
  • Rodríguez, E.M., Medesani, D.A., López Greco, L.S., Fingerman, M., Effects of some steroids and other compounds on ovarian growth of the swamp crayfish, Procambarus clarkii, during early vitellogenesis (2002) J. Exp. Zool., 292, pp. 82-87
  • Sarojini, R., Nagabhushanam, R., Fingerman, M., Mode of action of the neurotransmitter 5-hydroxytryptamine in stimulating ovarian maturation in the red swamp crayfish, Procambarus clarkii: An in vivo and in vitro study (1995) J. Exp. Zool., 271, pp. 395-400
  • Shih, J.T., Sex steroid-like substances in the ovaries, hepatopancreas, and body fluid of female Mictyris brevidactylus (1997) Zool. Stud., 36, pp. 136-145
  • Sokal, R.R., Rohlf, F.J., (1981) Biometry, Second Ed., , Freeman, New York
  • Tsukimura, B., Kamemoto, F.I., In vitro stimulation of oocytes by presumptive mandibular organ secretions in the shrimp, Penaeus vannamei (1991) Aquaculture, 92, pp. 59-66
  • Wainwright, G., Rees, H.H., Hormonal regulation of reproductive development in crustaceans (2001) Environment and Animal Development, pp. 71-84. , D. Atkinson, M. Thorndyke. (Eds.), Bios Scientific Publishers Ltd., New York
  • Webster, S.G., Neuropeptides inhibiting growth and reproduction in crustaceans (1998) Recent Advances in Arthropod Endocrinology, pp. 33-52. , G.M. Coast, S.G. Webster (Eds.), Cambridge University Press, Cambridge
  • Winer, B.J., (1971) Statistical Principles in Experimental Design, Second Ed., , MacGraw Hill, New York
  • Yano, I., Endocrine control of reproductive maturation in penaeid shrimp (2000) Recent Advances in Marine Biotechnology, Vol. 4: Aquaculture, Part A, 4, pp. 161-176. , M. Fingerman and R. Nagabhushanam (Eds.), Science Publishers Inc., Enfield, New Hampshire
  • Zapata, V., López Greco, L.S., Medesani, D., Rodríguez, E.M., Ovarian growth in the crab Chasmagnathus granulata induced by hormone and neuroregulators throughout the year. in vivo and in vitro studies (2003) Aquaculture, 224, pp. 339-352

Citas:

---------- APA ----------
Medesani, D.A., López Greco, L.S. & Rodríguez, E.M. (2004) . Interference of cadmium and copper with the endocrine control of ovarian growth, in the estuarine crab Chasmagnathus granulata. Aquatic Toxicology, 69(2), 165-174.
http://dx.doi.org/10.1016/j.aquatox.2004.05.003
---------- CHICAGO ----------
Medesani, D.A., López Greco, L.S., Rodríguez, E.M. "Interference of cadmium and copper with the endocrine control of ovarian growth, in the estuarine crab Chasmagnathus granulata" . Aquatic Toxicology 69, no. 2 (2004) : 165-174.
http://dx.doi.org/10.1016/j.aquatox.2004.05.003
---------- MLA ----------
Medesani, D.A., López Greco, L.S., Rodríguez, E.M. "Interference of cadmium and copper with the endocrine control of ovarian growth, in the estuarine crab Chasmagnathus granulata" . Aquatic Toxicology, vol. 69, no. 2, 2004, pp. 165-174.
http://dx.doi.org/10.1016/j.aquatox.2004.05.003
---------- VANCOUVER ----------
Medesani, D.A., López Greco, L.S., Rodríguez, E.M. Interference of cadmium and copper with the endocrine control of ovarian growth, in the estuarine crab Chasmagnathus granulata. Aquatic Toxicol. 2004;69(2):165-174.
http://dx.doi.org/10.1016/j.aquatox.2004.05.003