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Abstract:

Carbamate insecticides such as carbaryl and organophosphates such as azinphos-methyl share the ability to inhibit the activity of B-esterases. This study aimed to (1) assess the inhibitory effects of carbaryl on B-esterase activity in soft tissues and hemolymph of Planorbarius corneus; (2) establish whether binary mixtures of carbaryl and azinphos-methyl depart or not from a model of concentration addition on the inhibition of cholinesterase activity; (3) determine the bioconcentration and elimination of the pesticides. The results showed that exposure of gastropods to increasing concentrations of carbaryl (0.1–5 mg L−1) for 48 h inhibited cholinesterase activity in a concentration-dependent manner, with an EC50 of 1.4 ± 0.3 mg L−1 and 1.2 ± 0.1 mg L−1 for soft tissue and hemolymph, respectively. Carboxylesterase activity, measured with the substrates p-nitrophenyl butyrate and p-nitrophenyl acetate, was between 2.3 and 25 times more sensitive to carbaryl inhibition than cholinesterase activity. Binary mixtures corresponding to 0.5 EC50 carbaryl + 0.5 EC50 azinphos-methyl and 0.75 EC50 carbaryl + 0.75 EC50 azinphos-methyl produced inhibitions of cholinesterase activity similar to those of individual pesticides, following a model of concentration addition. Bioconcentration was analyzed using a one-compartment model. The absorption kinetics (k1) for both pesticides alone (1.4 mg L−1 of carbaryl or 1.8 mg L−1 of azinphos-methyl) or mixed (1.4 mg L−1 of carbaryl + 1.8 mg L−1 of azinphos-methyl) were similar. The elimination kinetics ratio (k2) estimated for the pesticides alone or in the mixtures showed that carbaryl was eliminated 3.5 times faster than azinphos-methyl. These results suggest that exposure of Planorbarius corneus to binary mixtures of carbaryl and azinphos-methyl for 48 h follow a concentration addition model on inhibition of cholinesterase activity and that the pesticide mixtures do not change the toxicokinetic parameters of the parent compounds. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:Toxicokinetic and toxicodynamic studies of carbaryl alone or in binary mixtures with azinphos methyl in the freshwater gastropod Planorbarius corneus
Autor:Cacciatore, L.C.; Verrengia Guerrero, N.R.; Cochón, A.C.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Ciudad Universitaria, Nuñez, Buenos Aires, 1428, Argentina
Palabras clave:Anticholinesterase insecticides; B-esterases; Mixtures; Planorbarius corneus; Toxicokinetics; azinphos methyl; carbaril; carboxylesterase; cholinesterase; azinphos methyl; carbaril; carboxylesterase; cholinesterase; fresh water; absorption; azinphos methyl; bioaccumulation; carbaryl; enzyme activity; freshwater environment; gastropod; inhibition; reaction kinetics; toxicology; absorption; adult; animal experiment; animal tissue; Article; cholinesterase inhibition; controlled study; EC50; ecotoxicity; enzyme activity; enzyme inhibition; hemolymph; nonhuman; Planorbarius corneus; priority journal; snail; toxicokinetics; animal; drug effect; enzymology; gastropod; kinetics; metabolism; toxicity; water pollutant; Gastropoda; Planorbarius corneus; Animals; Azinphosmethyl; Carbaryl; Carboxylesterase; Cholinesterases; Fresh Water; Gastropoda; Hemolymph; Kinetics; Toxicokinetics; Water Pollutants, Chemical
Año:2018
Volumen:199
Página de inicio:276
Página de fin:284
DOI: http://dx.doi.org/10.1016/j.aquatox.2018.04.005
Título revista:Aquatic Toxicology
Título revista abreviado:Aquat. Toxicol.
ISSN:0166445X
CODEN:AQTOD
CAS:azinphos methyl, 86-50-0; carbaril, 63-25-2; carboxylesterase, 59536-71-9, 83380-83-0, 9016-18-6, 9028-01-7; cholinesterase, 9001-08-5; Azinphosmethyl; Carbaryl; Carboxylesterase; Cholinesterases; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v199_n_p276_Cacciatore

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Citas:

---------- APA ----------
Cacciatore, L.C., Verrengia Guerrero, N.R. & Cochón, A.C. (2018) . Toxicokinetic and toxicodynamic studies of carbaryl alone or in binary mixtures with azinphos methyl in the freshwater gastropod Planorbarius corneus. Aquatic Toxicology, 199, 276-284.
http://dx.doi.org/10.1016/j.aquatox.2018.04.005
---------- CHICAGO ----------
Cacciatore, L.C., Verrengia Guerrero, N.R., Cochón, A.C. "Toxicokinetic and toxicodynamic studies of carbaryl alone or in binary mixtures with azinphos methyl in the freshwater gastropod Planorbarius corneus" . Aquatic Toxicology 199 (2018) : 276-284.
http://dx.doi.org/10.1016/j.aquatox.2018.04.005
---------- MLA ----------
Cacciatore, L.C., Verrengia Guerrero, N.R., Cochón, A.C. "Toxicokinetic and toxicodynamic studies of carbaryl alone or in binary mixtures with azinphos methyl in the freshwater gastropod Planorbarius corneus" . Aquatic Toxicology, vol. 199, 2018, pp. 276-284.
http://dx.doi.org/10.1016/j.aquatox.2018.04.005
---------- VANCOUVER ----------
Cacciatore, L.C., Verrengia Guerrero, N.R., Cochón, A.C. Toxicokinetic and toxicodynamic studies of carbaryl alone or in binary mixtures with azinphos methyl in the freshwater gastropod Planorbarius corneus. Aquat. Toxicol. 2018;199:276-284.
http://dx.doi.org/10.1016/j.aquatox.2018.04.005