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Abstract:

Atrazine, a widely use herbicide, has been classified as a potential endocrine disruptor, especially for freshwater species. In this study, we tested the hypothesis that atrazine can affect reproduction in crayfish through dysregulation of vitellogenin expression and hormone synthesis. Adult female crayfish (Procambarus clarkii) were exposed during one month to atrazine at concentrations of either 1 or 5 mg/L. At the end of the exposure, ovaries, hepatopancreas, and hemolymph samples were harvested for analysis of vitellogenin expression and steroid hormone levels. Ovarian tissue was also sampled for both biochemical and histological analyses. Our results show that atrazine-exposed crayfish had a lower expression of vitellogenin in the ovary and hepatopancreas, as well as smaller oocytes, and reduced vitellogenin content in the ovary. Despite these effects, circulating levels of estradiol increased in females exposed to 5 mg/L of atrazine, showing that the inhibiting effect of atrazine on vitellogenin production was not related to a lower secretion of sexual steroids. Instead, some early stimulating effects of estradiol on vitellogenesis could have occurred, particularly in the hepatopancreas. On the other hand, atrazine caused a higher metabolic effort, in terms of lactate production, presumably triggered to provide the energy needed to face the unspecific stress produced by the herbicide. Lipid peroxidation was not affected by atrazine, but glutathione levels were significantly increased. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii
Autor:Silveyra, G.R.; Silveyra, P.; Vatnick, I.; Medesani, D.A.; Rodríguez, E.M.
Filiación:Dept. of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, Ciudad Universitaria, Pab. II, Buenos Aires, C1428EGA, Argentina
Pulmonary Immunology and Physiology Laboratory, Dept. of Pediatrics, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, United States
Dept. of Biology, Widener University, Chester, PA 19809, United States
Palabras clave:Atrazine; Crayfish; Lipid peroxidation; Ovary; Sexual steroids; Vitellogenin; atrazine; glutathione; steroid; thiobarbituric acid reactive substance; vitellogenin; atrazine; estradiol; glucose; herbicide; lactic acid; messenger RNA; steroid; testosterone; thiobarbituric acid reactive substance; vitellogenin; atrazine; crayfish; endocrine disruptor; histology; hormone; lipid; pollution effect; pollution exposure; reproductive biology; steroid; animal tissue; Article; controlled study; environmental exposure; female; hemolymph; histopathology; hormone synthesis; lipid peroxidation; metabolic stress; nonhuman; priority journal; Procambarus clarkii; protein content; protein expression; reproductive toxicity; survival analysis; toxicity testing; vitellogenesis; animal; crayfish; drug effects; gene expression regulation; genetics; hepatopancreas; lipid peroxidation; metabolism; metabolome; oocyte; ovary; reproducibility; reproduction; toxicity; vitellogenesis; water pollutant; Astacoidea; Procambarus clarkii; Animals; Astacoidea; Atrazine; Estradiol; Female; Gene Expression Regulation; Glucose; Hemolymph; Hepatopancreas; Herbicides; Lactic Acid; Lipid Peroxidation; Metabolome; Oocytes; Ovary; Reproducibility of Results; Reproduction; RNA, Messenger; Steroids; Survival Analysis; Testosterone; Thiobarbituric Acid Reactive Substances; Vitellogenesis; Vitellogenins; Water Pollutants, Chemical
Año:2018
Volumen:197
Página de inicio:136
Página de fin:142
DOI: http://dx.doi.org/10.1016/j.aquatox.2018.02.017
Título revista:Aquatic Toxicology
Título revista abreviado:Aquat. Toxicol.
ISSN:0166445X
CODEN:AQTOD
CAS:atrazine, 1912-24-9; glutathione, 70-18-8; estradiol, 50-28-2; glucose, 50-99-7, 84778-64-3; lactic acid, 113-21-3, 50-21-5; testosterone, 58-22-0; Atrazine; Estradiol; Glucose; Herbicides; Lactic Acid; RNA, Messenger; Steroids; Testosterone; Thiobarbituric Acid Reactive Substances; Vitellogenins; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v197_n_p136_Silveyra

Referencias:

  • Alcorlo, P., Otero, M., Crehuet, M., Baltanás, A., Montes, C., The use of the red swamp crayfish (Procambarus clarkii, Girard) as indicator of the bioavailability of heavy metals in environmental monitoring in the River Guadiamar (SW, Spain) (2006) Sci. Total. Environ., 366, pp. 380-390
  • Álvarez, N.B., Avigliano, L., Mac Loughlin, C., Rodríguez, E.M., The adverse effect of the herbicide atrazine on the reproduction in the intertidal varunid crab Neohelice granulata (Dana, 1851) (2015) Reg. Stud. Mar. Sci., 1, pp. 1-6
  • American Public Health Association, American Water Works Association, Water Pollution Control Federation, Standard Methods for the Examination of Water and Wastewaters (2005), 21th ed. American Public Health Association Washington DC; Chang, E.S., Chang, S.A., Keller, R., Reddy, P.S., Snyder, M.J., Spees, J.L., Quantification of stress in lobsters: crustacean hyperglycemic hormone, stress protein and gene expression (1999) Am. Zool., 39, pp. 487-495
  • Charmantier, G., Charmantier-Daures, M., Van Herp, F., Hormonal regulation of growth and reproduction in crustaceans (1997) Recent Advances in Marine Biotechnology, vol. I, pp. 109-161. , M. Fingerman R. Nagabhushanam M.F. Thompson Oxford and IBH Publishing New Delhi
  • Clark, G.M., Goolsby, D.A., Occurrence and load of selected herbicides and metabolites in the lower Mississippi River (2000) Sci. Total Environ., 248, pp. 101-113
  • Coccia, E., De Lisa, E., Di Cristo, C., Di Cosmo, A., Paolucci, M., Effects of estradiol and progesterone on the reproduction of the freshwater crayfish Cherax albidus (2010) Biol. Bull., 218, pp. 36-47
  • Coupe, R.H., Thurman, E.M., Zimmerman, R., Relation of usage to the occurrence of cotton and rice herbicides in three streams of the Mississippi Delta (1998) Environ. Sci. Technol., 32, pp. 3673-3680
  • Crayfish production, Louisiana Crayfish Industry (2018), http://www.welovecrawfish.com/crawfish-industry.htm, (Accessed 5 February 2018); Dodson, S.I., Merritt, C., Shannahan, J., Shults, C., Low expo-sure concentrations of atrazine increase male production in Daphnia pulicaria (1999) Environ. Toxicol. Chem., 18, pp. 1568-1573
  • Dreon, M.S., Heras, H., Pollero, R.J., Metabolism of ovorubin, the major egg lipoprotein from the apple snail (2003) Mol. Cell. Biochem., 243, pp. 9-14
  • Fingerman, M., Nagabhushanam, R., Sarojini, R., Vertebrate-type hormones in crustaceans Localization, identification and functional significance (1993) Zool. Sci., 10, pp. 13-29
  • Fingerman, M., Endocrine mechanisms in crayfish, with emphasison reproduction and neurotransmitter regulation of hormonerelease (1995) Am. Zool., 35, pp. 68-78
  • Fingerman, M., Roles of neurotransmitters in regulating reproductive hormone release and gonadal maturation in decapod crustaceans (1997) Invertebr. Reprod. Dev., 31, pp. 47-54
  • García, F., Cunningham, M.L., Garda, H., Heras, H., Embryo lipoproteins and yolk lipovitellin consumption during embryogenesis in Macrobrachium borellii (Crustacea: Palaemonidae) (2008) Comp. Biochem. Physiol., 151B, pp. 317-322
  • Goretti, E., Pallottini, M., Ricciarini, M.I., Selvaggi, R., Cappelletti, D., Heavy metals bioaccumulation in selected tissues of red swamp crayfish: an easy tool for monitoring environmental contamination levels (2016) Sci. Total Environ., 559, pp. 339-346
  • Graymore, M., Stagnitti, F., Allinson, G., Impacts of atrazine in aquatic ecosystems (2001) Environ. Int., 26, pp. 483-495
  • Griboff, J., Morales, D., Bertrand, L., Bonansea, R.I., Monferrán, M.V., Asis, R., Wunderlin, D.A., Amé, M.V., Oxidative stress response induced by atrazine in Palaemonetes argentinus: the protective effect of vitamin E (2014) Ecotoxicol. Environ. Saf., 108, pp. 1-8
  • Hayes, T.B., Stuart, A.A., Mendoza, M., Collins, A., Noriega, N., Vonk, A., Johnston, G., Kpodzo, D., Characterization of atrazine-induced gonadal malformations in african clawed frogs (Xenopus laevis) and comparisons with effects of an androgen antagonist (cyproterone acetate) and exogenous estrogen (17β-estradiol): Support for the demasculinization/feminization hypothesis (2006) Environ. Health Perspect., 114, pp. 134-141
  • Huang, D.J., Wang, S.Y., Chen, H.C., Effects of the endocrine disrupter chemicals chlordane and lindane on the male green neon shrimp (Neocaridina denticulata) (2004) Chemosphere, 57, pp. 1621-1627
  • Huner, J.V., Barr, J.E., Red Swamp Crawfish: Biology and Exploitation (1991), Louisiana Sea Grant College Program Baton Rouge, Louisiana; Jablonowski, N.D., Schäffer, A., Burauel, P., Still present after all these years: persistence plus potential toxicity raise questions about the use of atrazine (2011) Environ. Sci. Poll. Res., 18, pp. 328-331
  • Jiang, H., Qian, Z., Lu, W., Ding, H., Yu, H., Wang, H., Li, J., Identification and characterization of reference genes for normalizing expression data from red swamp crawfish Procambarus clarkii (2015) Int. J. Mol. Sci., 16, pp. 21591-21605
  • Kulkarni, G.K., Glade, L., Fingerman, M., Oogenesis and effects of neuroendocrine tissues on in vitro synthesis of protein by the ovary of the red swamp crayfish Procambarus clarkii (Girard) (1991) J. Crust. Biol., 11, pp. 513-522
  • Lafont, R., Mathieu, M., Steroids in aquatic invertebrates (2007) Ecotoxicology, 16, pp. 109-130
  • Lascombe, I., Beffa, D., Ruegg, U., Tarradellas, J., Wahli, W., Estrogenicactivity assessment of environmental chemicals using in vitro assays: identification of two new estrogenic compounds (2000) Environ. Health Perspect., 108, pp. 621-629
  • Mac Loughlin, C., Canosa, I.S., Silveyra, G.R., López Greco, L.S., Rodríguez, E.M., Effects of atrazine on growth and sex differentiation: in juveniles of the freshwater crayfish Cherax quadricarinatus (2016) Ecotoxicol. Environ. Saf., 131, pp. 96-103
  • McClain, W.R., Romaire, R.P., Contribution of different food supplements to growth and production of red swamp crayfish (2009) Aquaculture, 294, pp. 93-98
  • McKinlay, R., Plant, J.A., Bell, J., Voulvoulis, N., Endocrine disrupting pesticides: implications for risk assessment (2008) Environ. Int., 34, pp. 168-183
  • Mirheydari, S.M., Paolucci, M., Matinfar, A., Ghasemi, S., Fluctuations of vertebrate-like steroids in the hemolymph of narrow-clawed crayfish, Astacus leptodactylus (Eschscholtz, 1823) (2013) Freshw. Crayfish, 19, pp. 237-241
  • Mirheydari, S.M., Paolucci, M., Matinfar, A., Soltani, M., Kamali, A., Asadpour-Ousalou, Y., Occurrence of vertebrate-like steroids in the male narrow-clawed crayfish Astacus leptodactylus (Eschscholtz, 1823) from Iran during the annual reproductive Cycle (2014) Glob. Vet., 13, pp. 247-254
  • Moore, M.T., Lizotte, R.E., Jr., Knight, S.S., Smith, S., Jr., Cooper, C.M., Assessment of pesticide contamination in three Mississippi Delta oxbow lakes using Hyalella azteca (2007) Chemosphere, 67, pp. 2184-2191
  • Nagaraju, G.P.C., Reproductive regulators in decapod crustaceans: an overview (2011) J. Exp. Biol., 214, pp. 3-16
  • Palma, P., Palma, V.L., Matos, C., Fernandes, R.M., Bohn, A., Soares, A.M.V.M., Barbosa, I., Effects of atrazine and endosulfan sulphate on the ecdysteroid system of Daphnia magna (2009) Chemosphere, 74, pp. 676-681
  • Preechaphol, R., Klinbunga, S., Ponza, P., Menasveta, P., Isolation and characterization of progesterone receptor-related protein p23 (Pm-p23) differentially expressed during ovarian development of the giant tiger shrimp Penaeus monodon (2010) Aquaculture, 308, pp. S75-S82
  • Quinitio, E., Hara, A., Yamauchi, K., Nakao, S., Changes in the steroid hormone and vitellogenin levels during thegametogenic cycle of the giant tiger shrimp Penaeus monodon (1994) Comp. Biochem. Physiol., 109C, pp. 21-26
  • Rebich, R.A., Coupe, R.H., Thurman, E.M., Herbicide concentrations in the Mississippi River Basin—the importance of chloroacetanilide herbicide degradates (2004) Sci. Total Environ., 321, pp. 189-199
  • Rodríguez, E.M., Medesani, D.A., Pathological lesions in larvae hatched from ovigerous females of Chasmagnathus granulata (Decapoda, Brachyura) exposed to cadmium (1994) Experientia, 50, pp. 975-977
  • Santos, E.A., Keller, R., Regulation of circulating levels of the crustacean hyperglycemic hormone-evidence of a dual feedback control system (1993) J. Comp. Physiol., 163A, pp. 374-379
  • Santos, K.C., Martínez, C.B.R., Genotoxic and biochemical effects of atrazine and Roundups, alone and in combination, on the Asian clam Corbicula fluminea (2014) Ecotoxicol. Environ. Saf., 100, pp. 7-14
  • Shih, J.T., Tseng, S.S., Progesterone-like substance in ovary and hepatopancreas of Uca vocans borealis (1999) Zool. Stud., 38, pp. 458-465
  • Silveyra, G.R., Canosa, I.S., Rodríguez, E.M., Medesani, D.A., Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata (2017) Comp. Biochem. Physiol., 192C, pp. 1-6
  • Sokal, R.R., Rohlf, F.J., Biometry (1981), 2nd ed. Freeman New York; Sokolova, I.M., Frederich, M., Bagwe, R., Lannig, G., Sukhotin, A.A., Energy homeostasis as an integrative tool for assessing limits of environmental stress tolerance in aquatic invertebrates (2012) Mar. Environ. Res., 79, pp. 1-15
  • Swevers, L., Lambert, J.G.D., De Loof, A., Metabolism of vertebrate-like steroids by tissues of three crustacean species (1991) Comp. Biochem. Physiol., 99B, pp. 35-41
  • Thongbuakaew, T., Siangcham, T., Suwansa-ard, S., Elizur, A., Cummins, S.F., Sobhon, P., Sretarugsa, P., Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii (2016) Steroids, 107, pp. 149-160
  • Tillitt, D.E., Papoulias, D.M., Whyte, J.J., Richter, C.A., Atrazine reduces reproduction in fathead minnow (Pimephales promelas) (2010) Aquat. Toxicol., 99, pp. 149-159
  • USEPA, Reregistration Eligibility Science Chapter for Atrazine Environmental Fate and Effects Chapter (2002), Washington DC; Villeneuve, D.L., Blankenship, A.L., Giesy, J.P., Interactions between environmental xenobiotics and estrogen-mediated responses (1998) Toxicant-Receptor Interactions, pp. 69-99. , M.S. Denison W.G. Helferich Taylor and Francis Philadelphia
  • Vonberg, D., Vanderborght, J., Cremer, N., Pütz, T., Herbst, M., Vereecken, H., 20 years of long-term atrazine monitoring in a shallow aquifer in western Germany (2014) Water Res., 50, pp. 294-306
  • Warrier, S.R., Tirumalai, R., Subramonian, T., Occurence of vertebrate steroids, estradiol 17β and progesterone in the reprodcing females of the mud crab Scylla serrata (2001) Comp. Biochem. Physiol., 130A, pp. 283-294
  • Zapata, V., López Greco, L.S., Medesani, D.A., Rodrı́guez, E.M., Ovarian growth in the crab Chasmagnathus granulata, induced by hormones and neuroregulators throughout the year. In vivo and in vitro studies (2003) Aquaculture, 224, pp. 339-352

Citas:

---------- APA ----------
Silveyra, G.R., Silveyra, P., Vatnick, I., Medesani, D.A. & Rodríguez, E.M. (2018) . Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii. Aquatic Toxicology, 197, 136-142.
http://dx.doi.org/10.1016/j.aquatox.2018.02.017
---------- CHICAGO ----------
Silveyra, G.R., Silveyra, P., Vatnick, I., Medesani, D.A., Rodríguez, E.M. "Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii" . Aquatic Toxicology 197 (2018) : 136-142.
http://dx.doi.org/10.1016/j.aquatox.2018.02.017
---------- MLA ----------
Silveyra, G.R., Silveyra, P., Vatnick, I., Medesani, D.A., Rodríguez, E.M. "Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii" . Aquatic Toxicology, vol. 197, 2018, pp. 136-142.
http://dx.doi.org/10.1016/j.aquatox.2018.02.017
---------- VANCOUVER ----------
Silveyra, G.R., Silveyra, P., Vatnick, I., Medesani, D.A., Rodríguez, E.M. Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii. Aquat. Toxicol. 2018;197:136-142.
http://dx.doi.org/10.1016/j.aquatox.2018.02.017