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Endosulfan (ES), a persistent organochlorine pesticide, is widely used despite its toxicity to non-target animals. Upon reaching water bodies, ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the action of ES on fish reproductive axis has been hardly studied thus far. The aim of the present work was to assess the endocrine disruptive potential of endosulfan on the pituitary gonadotropins levels and on the testes function due to ES in the South American freshwater fish Cichlasoma dimerus, using in vitro and in vivo approaches. In vitro experiments showed that ES inhibited the LH-stimulated steroidogenesis in gonads; no change was observed in gonadotropins release from pituitaries in culture. Laboratory waterborne ES (0.1, 0.3 and 1 μg/L) exposure for two months caused decrease in βFSH pituitary content and γGT activity in the testes (Sertoli cell function marker). Testicular histology revealed pathologies such as scarce intermediate stages of spermatogenesis, release of immature germ cells into the lobular lumen, presence of foam cells and interstitial fibrosis. As FSH and FSH-mediated steroidogenesis regulate spermatogenesis and Sertoli cell function, the effect of ES on FSH could be responsible for the morphological alterations observed in testes. In vitro, ES disrupted steroidogenesis in gonads, therefore similar effects in vivo cannot be ruled out. Based on this evidence, ES exhibits an endocrine disruptive action on the reproductive axis of C. dimerus, causing disruption at the pituitary and/or at the gonad level. These effects could acquire ecological significance under prolonged exposure to the pesticide in nature. © 2012 Elsevier B.V.


Documento: Artículo
Título:Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)
Autor:Da Cuña, R.H.; Pandolfi, M.; Genovese, G.; Piazza, Y.; Ansaldo, M.; Lo Nostro, F.L.
Filiación:Laboratorio de Ecotoxicología Acuática DBBE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Ciudad Autónoma de Buenos Aires, C1033AAJ, Argentina
Laboratorio de Neuroendocrinología y Comportamiento DBBE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
Instituto Antártico Argentino, Dirección Nacional del Antártico, Ciudad Autónoma de Buenos Aires, C1010AAZ, Argentina
Palabras clave:Cichlid fish; Endocrine disruption; Endosulfan; Gonadotropins; Sex steroids; Testes pathology; endosulfan; follitropin beta subunit; gamma glutamyltransferase; gonadotropin; luteinizing hormone; endocrine disruptor; endosulfan; morphology; organochlorine; perciform; pesticide; reproductive effort; toxicity; animal cell; animal experiment; animal tissue; article; brain function; brain region; cell function; Cichlasoma dimerus; cichlid; controlled study; ecotoxicology; endocrine disease; environmental exposure; enzyme activity; foam cell; germ cell; gonadotropin release; histopathology; hormone action; hypophysis; in vitro study; in vivo study; morphological trait; nonhuman; priority journal; regulatory mechanism; reproductive toxicity; Sertoli cell; spermatogenesis; steroidogenesis; testis function; tissue culture; Animals; Cells, Cultured; Cichlids; Endosulfan; Female; Gonadotropins, Pituitary; Male; Ovary; Pituitary Gland; Testis; Water Pollutants, Chemical; South America; Animalia; Cichlasoma; Cichlidae; Perciformes
Página de inicio:299
Página de fin:305
Título revista:Aquatic Toxicology
Título revista abreviado:Aquat. Toxicol.
CAS:endosulfan, 115-29-7; gamma glutamyltransferase, 85876-02-4; gonadotropin, 63231-54-9; luteinizing hormone, 39341-83-8, 9002-67-9; Endosulfan, 115-29-7; Gonadotropins, Pituitary; Water Pollutants, Chemical


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---------- APA ----------
Da Cuña, R.H., Pandolfi, M., Genovese, G., Piazza, Y., Ansaldo, M. & Lo Nostro, F.L. (2013) . Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae). Aquatic Toxicology, 126, 299-305.
---------- CHICAGO ----------
Da Cuña, R.H., Pandolfi, M., Genovese, G., Piazza, Y., Ansaldo, M., Lo Nostro, F.L. "Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)" . Aquatic Toxicology 126 (2013) : 299-305.
---------- MLA ----------
Da Cuña, R.H., Pandolfi, M., Genovese, G., Piazza, Y., Ansaldo, M., Lo Nostro, F.L. "Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae)" . Aquatic Toxicology, vol. 126, 2013, pp. 299-305.
---------- VANCOUVER ----------
Da Cuña, R.H., Pandolfi, M., Genovese, G., Piazza, Y., Ansaldo, M., Lo Nostro, F.L. Endocrine disruptive potential of endosulfan on the reproductive axis of Cichlasoma dimerus (Perciformes, Cichlidae). Aquat. Toxicol. 2013;126:299-305.