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Abstract:

Amyloid β protein (Aβ) fibrillogenesis is considered one of the crucial steps of Alzheimer's disease (AD) pathogenesis. The effect of endogenous neuronal amyloid fibrils on memory processes is unknown. To investigate this issue, we first characterised the Aβ fibrillar aggregates secreted by cerebellar granule cells and then we evaluated the effect of neuronal fibrils on an invertebrate model of memory. An increase of fibril formation, assessed by Thioflavin T (ThT) fluorescence, was observed in the conditioned medium of apoptotic neurons during 48h of the apoptotic process. Moreover, the depolarisation-stimulated secretion of cerebellar granule cells contains monomers of endogenous Aβ, which undergo cell-free fibrillogenesis over several days of incubation. The pattern of single endogenous fibrils, examined by electron microscopy, was similar to that of synthetic Aβ while a tighter and more complex interfibrillar organization was observed in endogenous fibrils. The biological effect of neuronal fibrils was studied in a long-term memory (LTM) paradigm, namely the context-signal learning of the crab Chasmagnathus. Pre-training injection of neuronal fibril extract (protein concentration, 1μg/ml) induced amnesia in a dose-dependent manner. On the contrary, no effect on retention was observed with the administration of two orders higher doses (100μg/ml) of synthetic Aβ1-40. These results indicate that only naturally secreted fibrils, but not synthetic Aβ, clearly interfere with memory process. © 2003 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Neuronal fibrillogenesis: Amyloid fibrils from primary neuronal cultures impair long-term memory in the crab Chasmagnathus
Autor:Romano, A.; Serafino, A.; Krasnowska, E.; Ciotti, M.T.; Calissano, P.; Ruberti, F.; Galli, C.
Filiación:Lab. de Neurobio. de la Memoria, Departamento de Fisiología, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Ist. di Neurobio. e Med. Molecolare, Consiglio Nazionale delle Ricerche, Viale K. Marx 15, 00137 Rome, Italy
Palabras clave:Amyloid β protein; Long-term memory; Neuronal fibrillogenesis; amyloid beta protein; monomer; thioflavine; animal cell; apoptosis; article; cell free system; cell secretion; conditioning; controlled study; crab; electron microscopy; fluorescence; granule cell; invertebrate; learning; long term memory; memory; nerve cell; nerve cell culture; nerve fiber; nonhuman; priority journal; protein secretion; training
Año:2003
Volumen:147
Número:1-2
Página de inicio:73
Página de fin:82
DOI: http://dx.doi.org/10.1016/S0166-4328(03)00118-9
Título revista:Behavioural Brain Research
Título revista abreviado:Behav. Brain Res.
ISSN:01664328
CODEN:BBRED
CAS:amyloid beta protein, 109770-29-8; thioflavine, 2390-54-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01664328_v147_n1-2_p73_Romano

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Citas:

---------- APA ----------
Romano, A., Serafino, A., Krasnowska, E., Ciotti, M.T., Calissano, P., Ruberti, F. & Galli, C. (2003) . Neuronal fibrillogenesis: Amyloid fibrils from primary neuronal cultures impair long-term memory in the crab Chasmagnathus. Behavioural Brain Research, 147(1-2), 73-82.
http://dx.doi.org/10.1016/S0166-4328(03)00118-9
---------- CHICAGO ----------
Romano, A., Serafino, A., Krasnowska, E., Ciotti, M.T., Calissano, P., Ruberti, F., et al. "Neuronal fibrillogenesis: Amyloid fibrils from primary neuronal cultures impair long-term memory in the crab Chasmagnathus" . Behavioural Brain Research 147, no. 1-2 (2003) : 73-82.
http://dx.doi.org/10.1016/S0166-4328(03)00118-9
---------- MLA ----------
Romano, A., Serafino, A., Krasnowska, E., Ciotti, M.T., Calissano, P., Ruberti, F., et al. "Neuronal fibrillogenesis: Amyloid fibrils from primary neuronal cultures impair long-term memory in the crab Chasmagnathus" . Behavioural Brain Research, vol. 147, no. 1-2, 2003, pp. 73-82.
http://dx.doi.org/10.1016/S0166-4328(03)00118-9
---------- VANCOUVER ----------
Romano, A., Serafino, A., Krasnowska, E., Ciotti, M.T., Calissano, P., Ruberti, F., et al. Neuronal fibrillogenesis: Amyloid fibrils from primary neuronal cultures impair long-term memory in the crab Chasmagnathus. Behav. Brain Res. 2003;147(1-2):73-82.
http://dx.doi.org/10.1016/S0166-4328(03)00118-9