Artículo
Andrei, G.M.; Damonte, E.B.; de Torres, R.A.; Coto, C.E. "Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L" (1988) Antiviral Research. 9(4):221-231
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Abstract:
A partially purified plant inhibitor (Meliacin) isolated from Melia azedarach L induced in cells a refractory state to virus infection. Meliacin was active in a large variety of continuous and/or primary cell cultures. A state of maximum virus resistance was achieved after 2 h of incubation and was maintained for at least 15 h; later on it declined but it was fully regained after a second pulse of Meliacin. Interferon was not detected in the supernatant of cells treated with Meliacin and a measurable increase in ds-RNA dependent protein kinase activity was not observed in extracts of Meliacin-treated cells. The antiviral state was not transferred by either extracellular fluid or direct cell-to-cell contact. An active cell metabolism was required for Meliacin action, which was partially reversed in the presence of actinomycin D. It appears that Meliacin is not an interferon-like substance, which induces an antiviral state based on a still unexplained mechanism. © 1988.
Registro:
| Documento: | Artículo |
| Título: | Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L |
| Autor: | Andrei, G.M.; Damonte, E.B.; de Torres, R.A.; Coto, C.E. |
| Filiación: | Laboratorio de Virología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina Departamento de Microbiología y Immunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina |
| Palabras clave: | Antiviral state; Melia azedarach L; Plant antiviral; dactinomycin; meliacine; unclassified drug; cell culture; chicken; drug mechanism; nonhuman; plant; plant leaf; priority journal; virus infection; Animal; Antiviral Agents; Cells, Cultured; Human; Interferons; Kinetics; Plants; Support, Non-U.S. Gov't; Vesicular stomatitis-Indiana virus; Virus Inhibitors; Virus Replication |
| Año: | 1988 |
| Volumen: | 9 |
| Número: | 4 |
| Página de inicio: | 221 |
| Página de fin: | 231 |
| DOI: | http://dx.doi.org/10.1016/0166-3542(88)90054-X |
| Título revista: | Antiviral Research |
| Título revista abreviado: | Antiviral Res. |
| ISSN: | 01663542 |
| CODEN: | ARSRD |
| CAS: | dactinomycin, 1402-38-6, 1402-58-0, 50-76-0; Antiviral Agents; Interferons, 9008-11-1; meliacin; Virus Inhibitors |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v9_n4_p221_Andrei |
Referencias:
- Andrei, Coto, de Torres, Antiviral activity and citotoxicity assays of crude and partial purified extracts of Melia azedarach L green leaves (1985) Rev. Arg. Microbiol., 17, pp. 187-194
- Andrei, Lampuri, Coto, de Torres, An antiviral factor from Melia azedarach L prevents Tacaribe virus encephalitis in mice (1986) Experientia, 42, pp. 843-845
- Blalock, Methods and procedures for experiments with mixed cell populations: transfer of the antiviral state induced by interferon (1981) Methods Enzymol., 79, pp. 353-359
- Chessin, Is there a plant interferon? (1983) Bot. Rev., 49, pp. 1-28
- De Maeyer, De Mayer-Guignard, (1979) Interferons, 15, pp. 205-293. , H. Fraenkel-Conrat, R.R. Wagner, Plenum Press, New York, London
- Ho, Armstrong, Interferon (1975) Ann. Rev. Microbiol., 29, pp. 131-161
- Kimchi, Shulman, Schimidt, Chenajovsky, Fradin, Revel, Kinetics of the induction of three-translation-regulatory enzymes by interferon (1979) Proc. Natl. Acad. Sci. U.S.A., 76, pp. 3208-3212
- Lengyel, Biochemistry of interferons and their actions (1982) Ann. Rev. Biochem., 51, pp. 251-282
- Moreno, Paveto, Passeron, Multiple protein kinase activities in the dimorphic fungus Mucor rouxii (1977) Arch. Biochem. Biophys., 180, pp. 225-231
- Wachsman, Damonte, Coto, de Torres, Antiviral effect of Melia azedarach L leaves extracts on Sindbis virus-infected cells (1987) Antiviral Res., 8, pp. 1-12
- Wachsman, Martino, Gutkind, Coussio, Coto, de Torres, Antiviral activity of Melia azedarach L plant extracts (1982) Fitoterapia, 53, pp. 167-170
Citas:
---------- APA ----------
Andrei, G.M., Damonte, E.B., de Torres, R.A. & Coto, C.E. (1988) . Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L. Antiviral Research, 9(4), 221-231.http://dx.doi.org/10.1016/0166-3542(88)90054-X
---------- CHICAGO ----------
Andrei, G.M., Damonte, E.B., de Torres, R.A., Coto, C.E. "Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L" . Antiviral Research 9, no. 4 (1988) : 221-231.http://dx.doi.org/10.1016/0166-3542(88)90054-X
---------- MLA ----------
Andrei, G.M., Damonte, E.B., de Torres, R.A., Coto, C.E. "Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L" . Antiviral Research, vol. 9, no. 4, 1988, pp. 221-231.http://dx.doi.org/10.1016/0166-3542(88)90054-X
---------- VANCOUVER ----------
Andrei, G.M., Damonte, E.B., de Torres, R.A., Coto, C.E. Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L. Antiviral Res. 1988;9(4):221-231.http://dx.doi.org/10.1016/0166-3542(88)90054-X
