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Abstract:

A selected group of aromatic disulfides, thiuram disulfides and thiosulfones, provided by the National Cancer Institute, were evaluated in vitro for their inhibitory activity against Junin virus (JUNV), the causative agent of Argentine hemorrhagic fever. The aromatic disulfides NSC4492 and NSC71033 and the thiuram disulfide NSC14560 were, respectively, the more potent virucidal and antiviral agents against JUNV, with inactivating concentration 50% (IC50) values of 0.2-0.5μM for virucidal compounds and antiviral effective concentration 50% (EC50) of 8.5μM for NSC14560. Both types of compounds exhibited inhibitory activity against three arenaviruses. Additionally, a comparable efficacy in the antiviral action of NSC14560 was observed in monkey, hamster or human cells with selectivity indices in the range 55.9-85.7. Time of addition experiments showed that the main antiviral activity of NSC14560 was situated before 5h of infection, but a significant inhibition was still observed when the compound was added up 9h p.i. This compound did not induce a refractory state to infection by cell pretreatment. Nor did it prevent viral entry, but the cytoplasmic and membrane expression of the main viral proteins was inhibited. The possible involvement of the RING finger motif of arenavirus Z protein as target for the thiuram disulfide is discussed. © 2010 Elsevier B.V.

Registro:

Documento: Artículo
Título:Inhibition of arenavirus infection by thiuram and aromatic disulfides
Autor:Sepúlveda, C.S.; García, C.C.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Antiviral; Arenavirus; Disulfides; Junin virus; Virucidal; aromatic disulfide; disulfide; disulfiram; fatty acid binding protein; nsc 112801; nsc 1339; nsc 14560; nsc 211; nsc 238936; nsc 255089; nsc 27320; nsc 27360; nsc 314654; nsc 317926; nsc 327174; nsc 342015; nsc 35825; nsc 38069; nsc 402561; nsc 4492; nsc 5239; nsc 58950; nsc 71033; nsc 76302; nsc 83217; nsc 93057; nsc 93058; ribavirin; unclassified drug; virus antigen; animal cell; antigen expression; antiviral activity; Arenavirus; arenavirus infection; Argentine hemorrhagic fever; article; cell strain BHK; controlled study; drug cytotoxicity; drug structure; human; human cell; IC 50; immunofluorescence; Junin virus; nonhuman; priority journal; RING finger motif; Vero cell; virucidal activity; virus attachment; virus entry; virus infectivity; virus inhibition; Animals; Antiviral Agents; Cell Line; Cercopithecus aethiops; Cricetinae; Disulfides; Humans; Inhibitory Concentration 50; Junin virus; Microbial Sensitivity Tests; Microbial Viability; Thiram; Virus Replication; Arenavirus; Cricetinae; Junin virus
Año:2010
Volumen:87
Número:3
Página de inicio:329
Página de fin:337
DOI: http://dx.doi.org/10.1016/j.antiviral.2010.06.005
Título revista:Antiviral Research
Título revista abreviado:Antiviral Res.
ISSN:01663542
CODEN:ARSRD
CAS:disulfide, 16734-12-6; disulfiram, 97-77-8; ribavirin, 36791-04-5; Antiviral Agents; Disulfides; Thiram, 137-26-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v87_n3_p329_Sepulveda

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Citas:

---------- APA ----------
Sepúlveda, C.S., García, C.C. & Damonte, E.B. (2010) . Inhibition of arenavirus infection by thiuram and aromatic disulfides. Antiviral Research, 87(3), 329-337.
http://dx.doi.org/10.1016/j.antiviral.2010.06.005
---------- CHICAGO ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B. "Inhibition of arenavirus infection by thiuram and aromatic disulfides" . Antiviral Research 87, no. 3 (2010) : 329-337.
http://dx.doi.org/10.1016/j.antiviral.2010.06.005
---------- MLA ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B. "Inhibition of arenavirus infection by thiuram and aromatic disulfides" . Antiviral Research, vol. 87, no. 3, 2010, pp. 329-337.
http://dx.doi.org/10.1016/j.antiviral.2010.06.005
---------- VANCOUVER ----------
Sepúlveda, C.S., García, C.C., Damonte, E.B. Inhibition of arenavirus infection by thiuram and aromatic disulfides. Antiviral Res. 2010;87(3):329-337.
http://dx.doi.org/10.1016/j.antiviral.2010.06.005