Artículo

Artuso, M.C.; Ellenberg, P.C.; Scolaro, L.A.; Damonte, E.B.; García, C.C. "Inhibition of Junín virus replication by small interfering RNAs" (2009) Antiviral Research. 84(1):31-37
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Abstract:

Junín virus (JUNV), the etiological agent of the Argentine hemorrhagic fever, has a single-stranded RNA genome with ambisense expression which encodes for five proteins. In previous works we have demonstrated that the Z arenavirus matrix protein represents an attractive target for antiviral therapy. With the aim of studying a new alternative therapeutic mechanism, four Z-specific siRNAs (Z1- to Z4-siRNAs) were tested showing variable efficacy. The most effective inhibitor was Z2-siRNA targeted at the region encompassed by nt 179-197 of Z gene. The efficacy of this Z2-siRNA against JUNV was also demonstrated in virus-infected cells, by testing infectious virus plaque formation (92.8% JUNV yield reduction), viral RNA level or antigen expression, as well as in cells transfected with Z-specific reporter plasmids (91% reduction in expression of Z-EGFP fusion protein). Furthermore, the lack of effect of this Z-siRNA on the expression of other JUNV proteins, such as N and GPC, confirmed the specificity of action exerted by Z2-siRNA on Z transcript. Thus, the present study represents the first report of virus inhibition mediated by RNA interference for a New World arenavirus. © 2009 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Inhibition of Junín virus replication by small interfering RNAs
Autor:Artuso, M.C.; Ellenberg, P.C.; Scolaro, L.A.; Damonte, E.B.; García, C.C.
Filiación:Laboratory of Virology, Department of Biological Chemistry, School of Sciences, Ciudad Universitaria, Pabellon 2, Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Arenavirus; Junín virus; Matrix protein; RNA interference; siRNAs; guanine nucleotide binding protein; small interfering RNA; virus antigen; virus RNA; animal cell; antigen expression; article; controlled study; drug efficacy; drug specificity; drug targeting; genetic transfection; human; human cell; Junin virus; nonhuman; nucleotide sequence; priority journal; protein expression; RNA interference; virus inhibition; virus plaque; virus replication; Animals; Arenaviridae Infections; Base Sequence; Cell Line; Cercopithecus aethiops; Cricetinae; Down-Regulation; Junin virus; Molecular Sequence Data; RNA Interference; RNA, Small Interfering; Sequence Alignment; Vero Cells; Virus Replication; Arenavirus; Junin virus
Año:2009
Volumen:84
Número:1
Página de inicio:31
Página de fin:37
DOI: http://dx.doi.org/10.1016/j.antiviral.2009.07.001
Título revista:Antiviral Research
Título revista abreviado:Antiviral Res.
ISSN:01663542
CODEN:ARSRD
CAS:RNA, Small Interfering
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v84_n1_p31_Artuso

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Citas:

---------- APA ----------
Artuso, M.C., Ellenberg, P.C., Scolaro, L.A., Damonte, E.B. & García, C.C. (2009) . Inhibition of Junín virus replication by small interfering RNAs. Antiviral Research, 84(1), 31-37.
http://dx.doi.org/10.1016/j.antiviral.2009.07.001
---------- CHICAGO ----------
Artuso, M.C., Ellenberg, P.C., Scolaro, L.A., Damonte, E.B., García, C.C. "Inhibition of Junín virus replication by small interfering RNAs" . Antiviral Research 84, no. 1 (2009) : 31-37.
http://dx.doi.org/10.1016/j.antiviral.2009.07.001
---------- MLA ----------
Artuso, M.C., Ellenberg, P.C., Scolaro, L.A., Damonte, E.B., García, C.C. "Inhibition of Junín virus replication by small interfering RNAs" . Antiviral Research, vol. 84, no. 1, 2009, pp. 31-37.
http://dx.doi.org/10.1016/j.antiviral.2009.07.001
---------- VANCOUVER ----------
Artuso, M.C., Ellenberg, P.C., Scolaro, L.A., Damonte, E.B., García, C.C. Inhibition of Junín virus replication by small interfering RNAs. Antiviral Res. 2009;84(1):31-37.
http://dx.doi.org/10.1016/j.antiviral.2009.07.001