Artículo
Castilla, V.; Larzábal, M.; Sgalippa, N.A.; Wachsman, M.B.; Coto, C.E. "Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication" (2005) Antiviral Research. 68(2):88-95
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Abstract:
The antiviral mode of action of the synthetic brassinosteroid (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (6b) against Junin virus replication in Vero cells was investigated. Time-related experiments showed that 6b mainly affects an early event of virus growth cycle. Neither adsorption nor internalization of viral particles was the target of the inhibitory action. The analysis of the effect of 6b on viral RNA synthesis demonstrated that the presence of the compound adversely affects virus RNA replication by preventing the synthesis of full length antigenomic RNA. Although 6b was most effective the earlier it was added to the cells after infection with JV, a high level of inhibition of JV yield and fusion activity of newly synthesized viral glycoproteins was still detected when the compound was present during the last hours of infection. Therefore, we cannot rule out an inhibitory action of 6b on later events of JV replicative cycle. © 2005 Elsevier B.V. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication |
| Autor: | Castilla, V.; Larzábal, M.; Sgalippa, N.A.; Wachsman, M.B.; Coto, C.E. |
| Filiación: | Laboratorio de Virología, Departamento de Química Biológica, Pabellón 2, 1428 Buenos Aires, Argentina |
| Palabras clave: | Antiviral activity; Arenavirus; Brassinosteroid; Hemorrhagic fever virus; 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one; brassinosteroid; genomic RNA; unclassified drug; virus glycoprotein; virus RNA; animal cell; antiviral activity; article; controlled study; drug mechanism; drug synthesis; Junin virus; nonhuman; priority journal; RNA synthesis; Vero cell; virogenesis; virus particle; virus replication; Animals; Antiviral Agents; Cell Fusion; Cell Line; Cercopithecus aethiops; Cholestanones; Cricetinae; DNA, Complementary; Giant Cells; Immunoprecipitation; Junin virus; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Vero Cells; Viral Proteins; Virus Replication; Animalia; Arenavirus; Junin virus |
| Año: | 2005 |
| Volumen: | 68 |
| Número: | 2 |
| Página de inicio: | 88 |
| Página de fin: | 95 |
| DOI: | http://dx.doi.org/10.1016/j.antiviral.2005.07.007 |
| Título revista: | Antiviral Research |
| Título revista abreviado: | Antiviral Res. |
| ISSN: | 01663542 |
| CODEN: | ARSRD |
| CAS: | (22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one; Antiviral Agents; Cholestanones; DNA, Complementary; RNA, Viral; Viral Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v68_n2_p88_Castilla |
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Citas:
---------- APA ----------
Castilla, V., Larzábal, M., Sgalippa, N.A., Wachsman, M.B. & Coto, C.E. (2005) . Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication. Antiviral Research, 68(2), 88-95.http://dx.doi.org/10.1016/j.antiviral.2005.07.007
---------- CHICAGO ----------
Castilla, V., Larzábal, M., Sgalippa, N.A., Wachsman, M.B., Coto, C.E. "Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication" . Antiviral Research 68, no. 2 (2005) : 88-95.http://dx.doi.org/10.1016/j.antiviral.2005.07.007
---------- MLA ----------
Castilla, V., Larzábal, M., Sgalippa, N.A., Wachsman, M.B., Coto, C.E. "Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication" . Antiviral Research, vol. 68, no. 2, 2005, pp. 88-95.http://dx.doi.org/10.1016/j.antiviral.2005.07.007
---------- VANCOUVER ----------
Castilla, V., Larzábal, M., Sgalippa, N.A., Wachsman, M.B., Coto, C.E. Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication. Antiviral Res. 2005;68(2):88-95.http://dx.doi.org/10.1016/j.antiviral.2005.07.007
