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In the present study, the protective effect of 1T1, a λ-carrageenan extracted from the red seaweed Gigartina skottsbergii was evaluated in a murine model of herpes simplex virus type 2 (HSV-2) genital infection. Six to eight-week-old female BALB/c mice were intravaginally inoculated with a lethal dose of HSV-2 (MS strain) and pre- or post-infection treated with different doses of a 10 mg/ml solution of 1T1. A single topical administration of 1T1 shortly before infection of BALB/c mice with HSV-2 protected 9 out of 10 mice from HSV-2-induced lesions and mortality, compared with only 10% survival in control mice. In addition, 1T1 produced a total blockade in virus shedding in the vaginal secretions. When 1T1 pre-treatment was reinforced with a second dose 2 h after infection, total protection was observed even when the prophylactic administration had taken place at 60 min before infection. The irreversible virucidal action of 1T1 against herpes virus seems to be responsible of its protective effect against virus replication and mortality following vaginal HSV-2 infection. © 2004 Elsevier B.V. All rights reserved.


Documento: Artículo
Título:Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice
Autor:Carlucci, M.J.; Scolaro, L.A.; Noseda, M.D.; Cerezo, A.S.; Damonte, E.B.
Filiación:Laboratorio de Virología, Depto. de Quím. Biol., Fac. Cie. Exact. Y Nat., Univ. B., Argentina
Departamento de Bioquímica, Univ. Federal Do Paraná, Curitiba, Brazil, Brazil
Depto. de Quím. Orgán., Fac. de Ciencias Exactas Y Naturales, Univ. Buenos Aires, Buenos Aires, A., Argentina
Palabras clave:Herpes simplex virus type 2; Natural carrageenan; Sexually transmitted disease; Vaginal infection; Virucidal activity; carrageenan; animal experiment; animal model; article; controlled study; dose response; drug effect; drug isolation; drug mechanism; female; genital herpes; Herpes simplex virus 2; infection prevention; inoculation; lethal dose; mortality; mouse; nonhuman; priority journal; seaweed; survival rate; time; vagina secretion; virus replication; virus shedding; virus strain; Animals; Antiviral Agents; Carrageenan; Cercopithecus aethiops; Female; Herpes Genitalis; Herpesvirus 2, Human; Mice; Mice, Inbred BALB C; Vagina; Vaginal Diseases; Vero Cells; Virus Shedding; algae; Gigartina; Gigartina skottsbergii; Herpesviridae; Human herpesvirus 2; Murinae; Simplexvirus
Página de inicio:137
Página de fin:141
Título revista:Antiviral Research
Título revista abreviado:Antiviral Res.
CAS:carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; Antiviral Agents; Carrageenan, 9000-07-1


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---------- APA ----------
Carlucci, M.J., Scolaro, L.A., Noseda, M.D., Cerezo, A.S. & Damonte, E.B. (2004) . Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice. Antiviral Research, 64(2), 137-141.
---------- CHICAGO ----------
Carlucci, M.J., Scolaro, L.A., Noseda, M.D., Cerezo, A.S., Damonte, E.B. "Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice" . Antiviral Research 64, no. 2 (2004) : 137-141.
---------- MLA ----------
Carlucci, M.J., Scolaro, L.A., Noseda, M.D., Cerezo, A.S., Damonte, E.B. "Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice" . Antiviral Research, vol. 64, no. 2, 2004, pp. 137-141.
---------- VANCOUVER ----------
Carlucci, M.J., Scolaro, L.A., Noseda, M.D., Cerezo, A.S., Damonte, E.B. Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice. Antiviral Res. 2004;64(2):137-141.