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Wachsman, M.B.; Castilla, V.; De Ruiz Holgado, A.P.; De Torres, R.A.; Sesma, F.; Coto, C.E. "Enterocin CRL35 inhibits late stages of HSV-1 and HSV-2 replication in vitro" (2003) Antiviral Research. 58(1):17-24
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Abstract:

The replication of herpes simplex virus (HSV) type 1 and 2 in Vero cells is inhibited in the presence of enterocin CRL35 (ECRL), a bacteriocin produced by Enterococcus faecium CRL35. Attempts to resolve the mode of action of ECRL indicate that virus adsorption and penetration are not affected. Instead, a late step of virus multiplication is hindered since the addition of 100μg/ml of ECRL at 8h post infection still causes a 90% inhibition of virus release. The effect of ECRL on HSV antigen expression was studied by immunofluorescence using a polyclonal serum and a monoclonal antibody against glycoprotein D (γ protein). These studies indicated that ECRL impeded the second round of infection, apparently as a consequence of the inhibition of glycoprotein D expression. The replication of syncytial mutants of HSV-1 was significantly inhibited at a ECRL concentration of 25μg/ml. Both the percentage of fused cells and the polykaryocyte size were affected. Studies on the effect of ECRL on viral protein synthesis showed that in the presence of ECRL, HSV late γ proteins were not synthesized. From these findings, it is concluded that inhibition of HSV spreading by ECRL is due to the prevention of mainly late glycoprotein synthesis. © 2003 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Enterocin CRL35 inhibits late stages of HSV-1 and HSV-2 replication in vitro
Autor:Wachsman, M.B.; Castilla, V.; De Ruiz Holgado, A.P.; De Torres, R.A.; Sesma, F.; Coto, C.E.
Filiación:Laboratorio de Virología, Depto. de Quím. Biol., Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina
Ctro. Referencia para Lactobacilos, Chacabuco 145, 4000 Tucuman, Argentina
Laboratorio de Microbiología, Fac. de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina
Palabras clave:Antiviral; Bacteriocin; Enterococcus faecium; Herpes simplex type 1 and 2; bacteriocin; enterocin crl35; glycoprotein D; late gamma protein; monoclonal antibody; unclassified drug; virus antigen; virus protein; animal cell; antigen expression; antiviral activity; article; cell fusion; cell size; concentration response; controlled study; drug activity; drug effect; Enterococcus faecium; herpes; Herpes simplex virus 1; Herpes simplex virus 2; nonhuman; priority journal; protein expression; protein synthesis inhibition; Vero cell; virus adsorption; virus inhibition; virus mutant; virus replication; Enterococcus; Enterococcus faecium; Human herpesvirus 1; Human herpesvirus 2; Simplexvirus
Año:2003
Volumen:58
Número:1
Página de inicio:17
Página de fin:24
DOI: http://dx.doi.org/10.1016/S0166-3542(02)00099-2
Título revista:Antiviral Research
Título revista abreviado:Antiviral Res.
ISSN:01663542
CODEN:ARSRD
CAS:bacteriocin, 11047-01-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v58_n1_p17_Wachsman

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Citas:

---------- APA ----------
Wachsman, M.B., Castilla, V., De Ruiz Holgado, A.P., De Torres, R.A., Sesma, F. & Coto, C.E. (2003) . Enterocin CRL35 inhibits late stages of HSV-1 and HSV-2 replication in vitro. Antiviral Research, 58(1), 17-24.
http://dx.doi.org/10.1016/S0166-3542(02)00099-2
---------- CHICAGO ----------
Wachsman, M.B., Castilla, V., De Ruiz Holgado, A.P., De Torres, R.A., Sesma, F., Coto, C.E. "Enterocin CRL35 inhibits late stages of HSV-1 and HSV-2 replication in vitro" . Antiviral Research 58, no. 1 (2003) : 17-24.
http://dx.doi.org/10.1016/S0166-3542(02)00099-2
---------- MLA ----------
Wachsman, M.B., Castilla, V., De Ruiz Holgado, A.P., De Torres, R.A., Sesma, F., Coto, C.E. "Enterocin CRL35 inhibits late stages of HSV-1 and HSV-2 replication in vitro" . Antiviral Research, vol. 58, no. 1, 2003, pp. 17-24.
http://dx.doi.org/10.1016/S0166-3542(02)00099-2
---------- VANCOUVER ----------
Wachsman, M.B., Castilla, V., De Ruiz Holgado, A.P., De Torres, R.A., Sesma, F., Coto, C.E. Enterocin CRL35 inhibits late stages of HSV-1 and HSV-2 replication in vitro. Antiviral Res. 2003;58(1):17-24.
http://dx.doi.org/10.1016/S0166-3542(02)00099-2