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Abstract:

Several disulfide-based compounds, including intermolecular aromatic disulfides of the type Ph-S-S-Ph and dithianes with the sulfur atoms tethered in a ring structure, have shown effective inhibitory activity against the arenaviruses Junin (JUNV), agent of Argentine hemorrhagic fever, and Tacaribe (TCRV). These compounds showed a strong virucidal effect with inactivating concentration 50% (IC50) values in the range 0.6-5.0 μM, and also were effective to reduce virus yields from infected cells. The mode of inactivating action of two active compounds, the aromatic bis disulfide NSC20625 and the dithiane NSC624152, was further studied. Both compounds were able to inactivate arenaviruses after a few minutes of direct contact with virions, in a concentration- and time-dependent manner. The ability of drug-treated virus to perform several steps of the replication cycle was analyzed. The killed virus particles were found to bind and enter to Vero cells with the same efficacy as infectious native virions, but the ability of inactivated virions to synthesize viral proteins in Vero cells was abolished. Thus, treatment of JUNV and TCRV with these compounds destroyed virion infectivity, generating particles which entered the host cell but were unable to complete the viral biosynthetic processes. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Mode of inactivation of arenaviruses by disulfide-based compounds
Autor:García, C.C; Candurra, N.A; Damonte, E.B
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Antiviral; Arenaviruses; Disulfides; Junin virus; Tacaribe virus; Virus inactivation; antivirus agent; disulfide; n 2 100 6; nsc 20625; nsc 4493; nsc 624125; nsc 624151; nsc 624152; nsc 677459; unclassified drug; virus protein; animal cell; Arenavirus; article; binding kinetics; concentration response; controlled study; IC 50; Junin virus; nonhuman; priority journal; protein synthesis; time; Vero cell; virion; virus inactivation; virus particle; virus replication; Animals; Antiviral Agents; Arenaviruses, New World; Cercopithecus aethiops; Disulfides; Junin virus; Vero Cells; Viral Proteins; Virion; Virus Replication; Argentina (fish); Junin virus; Tacaribe virus
Año:2002
Volumen:55
Número:3
Página de inicio:437
Página de fin:446
DOI: http://dx.doi.org/10.1016/S0166-3542(02)00076-1
Título revista:Antiviral Research
Título revista abreviado:Antiviral Res.
ISSN:01663542
CODEN:ARSRD
CAS:Antiviral Agents; Disulfides; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v55_n3_p437_Garcia

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Citas:

---------- APA ----------
García, C.C, Candurra, N.A & Damonte, E.B (2002) . Mode of inactivation of arenaviruses by disulfide-based compounds. Antiviral Research, 55(3), 437-446.
http://dx.doi.org/10.1016/S0166-3542(02)00076-1
---------- CHICAGO ----------
García, C.C, Candurra, N.A, Damonte, E.B. "Mode of inactivation of arenaviruses by disulfide-based compounds" . Antiviral Research 55, no. 3 (2002) : 437-446.
http://dx.doi.org/10.1016/S0166-3542(02)00076-1
---------- MLA ----------
García, C.C, Candurra, N.A, Damonte, E.B. "Mode of inactivation of arenaviruses by disulfide-based compounds" . Antiviral Research, vol. 55, no. 3, 2002, pp. 437-446.
http://dx.doi.org/10.1016/S0166-3542(02)00076-1
---------- VANCOUVER ----------
García, C.C, Candurra, N.A, Damonte, E.B. Mode of inactivation of arenaviruses by disulfide-based compounds. Antiviral Res. 2002;55(3):437-446.
http://dx.doi.org/10.1016/S0166-3542(02)00076-1