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Abstract:

Trifluoperazine (TFP) and chlorpromazine (CPZ), two pharmacologically active phenothiazine derivatives, were evaluated for their inhibitory activity on the replication of the arenaviruses Junin (JV), the etiological agent of Argentine hemorrhagic fever, Tacaribe virus and Pichinde virus. Both compounds achieved a concentration-dependent inhibition of viral multiplication at concentrations not affecting cell viability. The 50% inhibitory concentration (IC50) values determined by a virus yield inhibition assay for several strains of JV, including a human pathogenic strain, were in the range of 7.7-23.0 μM and the 90% inhibitory concentration (IC90) fluctuated between 16.6 and 35.2 μM. From time of addition and removal experiments, it can be concluded that CPZ inhibited an early stage in the replicative cycle of JV, probably viral entry. TFP also affected JV penetration when present soon after virus adsorption, and also interfered with a later step of viral maturation when added after 7 h of infection. The expression of viral antigens in the cytoplasm of infected cells was highly reduced in the presence of the compounds, as revealed by immunofluorescence staining, whereas no JV proteins were detected at the cell membrane. The distribution pattern of viral proteins was altered in the few cells exhibiting positive fluorescence after treatment with the phenothiazines. The TFP-induced inhibitory effect on JV multiplication was significantly reversed in the presence of 5 μM calmodulin. These data indicate that TFP and CPZ inhibit JV replication in vitro. Our findings suggest that the integrity of the actin microfilaments may be required for optimal arenavirus multiplication.

Registro:

Documento: Artículo
Título:Inhibition of arenavirus multiplication in vitro by phenothiazines
Autor:Candurra, N.A.; Maskin, L.; Damonte, E.B.
Filiación:Laboratorio de Virología, Depto. de Quimica Biológica, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Arenaviruses; Argentine hemorrhagic fever; Calmodulin antagonists; Junin virus; Phenothiazines; actin; calmodulin inhibitor; chlorpromazine; phenothiazine; trifluoperazine; animal cell; antiviral activity; Arenavirus; article; controlled study; in vitro study; Junin virus; nonhuman; priority journal; virus inhibition; Animalia; Arenavirus; Argentina (fish); Junin virus; Pichinde virus; Tacaribe virus
Año:1996
Volumen:31
Número:3
Página de inicio:149
Página de fin:158
DOI: http://dx.doi.org/10.1016/0166-3542(96)06956-2
Título revista:Antiviral Research
Título revista abreviado:ANTIVIRAL RES.
ISSN:01663542
CODEN:ARSRD
CAS:chlorpromazine, 50-53-3, 69-09-0; phenothiazine, 92-84-2; trifluoperazine, 117-89-5, 440-17-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v31_n3_p149_Candurra

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Citas:

---------- APA ----------
Candurra, N.A., Maskin, L. & Damonte, E.B. (1996) . Inhibition of arenavirus multiplication in vitro by phenothiazines. Antiviral Research, 31(3), 149-158.
http://dx.doi.org/10.1016/0166-3542(96)06956-2
---------- CHICAGO ----------
Candurra, N.A., Maskin, L., Damonte, E.B. "Inhibition of arenavirus multiplication in vitro by phenothiazines" . Antiviral Research 31, no. 3 (1996) : 149-158.
http://dx.doi.org/10.1016/0166-3542(96)06956-2
---------- MLA ----------
Candurra, N.A., Maskin, L., Damonte, E.B. "Inhibition of arenavirus multiplication in vitro by phenothiazines" . Antiviral Research, vol. 31, no. 3, 1996, pp. 149-158.
http://dx.doi.org/10.1016/0166-3542(96)06956-2
---------- VANCOUVER ----------
Candurra, N.A., Maskin, L., Damonte, E.B. Inhibition of arenavirus multiplication in vitro by phenothiazines. ANTIVIRAL RES. 1996;31(3):149-158.
http://dx.doi.org/10.1016/0166-3542(96)06956-2