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Abstract:

The efficacy of several antibiotic treatments to eliminate mycoplasma from Vero cells contaminated chronically with Mycoplasma orale II were tested. Minocyclin, Kanamycin, Tylosine and Roxitromycin, at non cytotoxic concentrations, were assayed alone or in different combinations. Mycoplasma contamination was effectively eradicated without recurrence once the following regime was applied: Incubation of contaminated cells with Tylosine (250 μg/ml) for 12 days followed by incubation with Minocycline (5 μg/ml) for 10 days. This treatment was not deletereus for cell growth, it was effective after only one application and it was successful to eradicate mycoplasma from other contaminated eukaryotic continuous cell lines. © 1994.

Registro:

Documento: Artículo
Título:A simple method to eliminate mycoplasma from cell cultures
Autor:Coronato, S.; Vullo, D.; Coto, C.E.
Filiación:Laboratorio de Virologia, Dto. de Quimica Biológica, Facultad de Cieneias Exact as y Naturales, University of Buenos Aires, Pabellón 2, Piso 4, Ciudad Universitaria, 1428 Buenos AiresArgentina
Palabras clave:Antibiotic treatment; Cell lines; Contaminated cells; Mycoplasma; antibiotic agent; kanamycin; minocycline; roxithromycin; tylosin; animal cell; article; cell culture; contamination; drug efficacy; mycoplasma; nonhuman; priority journal; Animal; Antibiotics, Combined; Cells, Cultured; Cercopithecus aethiops; Drug Resistance, Microbial; Eukaryotic Cells; Hamsters; Human; Kanamycin; Minocycline; Mycoplasma; Roxithromycin; Tissue Culture; Tumor Cells, Cultured; Tylosin; Animalia; Eukaryota; Mycoplasma; Mycoplasma orale
Año:1994
Volumen:46
Número:1
Página de inicio:85
Página de fin:94
DOI: http://dx.doi.org/10.1016/0166-0934(94)90018-3
Título revista:Journal of Virological Methods
Título revista abreviado:J. Virol. Methods
ISSN:01660934
CODEN:JVMED
CAS:Antibiotics, Combined; Kanamycin, 59-01-8; Minocycline, 10118-90-8; Roxithromycin, 80214-83-1; Tylosin, 1401-69-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v46_n1_p85_Coronato

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Citas:

---------- APA ----------
Coronato, S., Vullo, D. & Coto, C.E. (1994) . A simple method to eliminate mycoplasma from cell cultures. Journal of Virological Methods, 46(1), 85-94.
http://dx.doi.org/10.1016/0166-0934(94)90018-3
---------- CHICAGO ----------
Coronato, S., Vullo, D., Coto, C.E. "A simple method to eliminate mycoplasma from cell cultures" . Journal of Virological Methods 46, no. 1 (1994) : 85-94.
http://dx.doi.org/10.1016/0166-0934(94)90018-3
---------- MLA ----------
Coronato, S., Vullo, D., Coto, C.E. "A simple method to eliminate mycoplasma from cell cultures" . Journal of Virological Methods, vol. 46, no. 1, 1994, pp. 85-94.
http://dx.doi.org/10.1016/0166-0934(94)90018-3
---------- VANCOUVER ----------
Coronato, S., Vullo, D., Coto, C.E. A simple method to eliminate mycoplasma from cell cultures. J. Virol. Methods. 1994;46(1):85-94.
http://dx.doi.org/10.1016/0166-0934(94)90018-3