Artículo
Abstract:
A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglutination (PH), passive hemagglutination inhibition (PHI) and passive immune hemolysis (PIL) assays. Fresh and SRBC stored in Alsever's solution showed similar behavior when used as indicator cells. Optimal sensitization of erythrocytes was achieved using tannic acid 1:20000 and 20 μg of purified virus/ml at pH 7.6. Specificity of the reaction was confirmed by PH and PHI in homologous and heterologous systems. The coupled antigen-antibody complex was sensitive to complement mediated lysis in a PIL test.
Registro:
| Documento: | Artículo |
| Título: | Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays |
| Autor: | Pujol, C.A.; Feledi, C.A.; Massouh, E.J. |
| Filiación: | Catedra de Inmunoquimica, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina |
| Idioma: | Inglés |
| Palabras clave: | virus antibody; virus antigen; cell culture; foot and mouth disease virus; hemagglutination; nonhuman; priority journal; serum; Animal; Antigens, Viral; Aphthovirus; Erythrocytes; Hemagglutination Inhibition Tests; Hemagglutination Tests; Hemolytic Plaque Technique; Immunologic Techniques; In Vitro; Microbiological Techniques; Sheep; Support, Non-U.S. Gov't; Tannic Acid |
| Año: | 1988 |
| Volumen: | 20 |
| Número: | 4 |
| Página de inicio: | 275 |
| Página de fin: | 284 |
| Título revista: | Journal of Virological Methods |
| Título revista abreviado: | J. VIROL. METHODS |
| ISSN: | 01660934 |
| CODEN: | JVMED |
| CAS: | Antigens, Viral; Tannic Acid |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v20_n4_p275_Pujol |
Citas:
---------- APA ----------
Pujol, C.A., Feledi, C.A. & Massouh, E.J. (1988) . Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays. Journal of Virological Methods, 20(4), 275-284.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v20_n4_p275_Pujol [ ]
---------- CHICAGO ----------
Pujol, C.A., Feledi, C.A., Massouh, E.J. "Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays" . Journal of Virological Methods 20, no. 4 (1988) : 275-284.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v20_n4_p275_Pujol [ ]
---------- MLA ----------
Pujol, C.A., Feledi, C.A., Massouh, E.J. "Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays" . Journal of Virological Methods, vol. 20, no. 4, 1988, pp. 275-284.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v20_n4_p275_Pujol [ ]
---------- VANCOUVER ----------
Pujol, C.A., Feledi, C.A., Massouh, E.J. Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays. J. VIROL. METHODS. 1988;20(4):275-284.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01660934_v20_n4_p275_Pujol [ ]
