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Abstract:

Medroxyprogesterone acetate (MPA)-induced mammary carcinomas express high levels of estrogen (ER) and progesterone receptors (PR) and when transplanted in syngeneic mice they show a progestin-dependent (PD) growth pattern. By successive transplantation, progestin-independent (PI) variants were generated and showed a different response to antihormone therapy. A diploid chromosome number (2n = 40) was found in three of five PD tumors, with numbers in the triploid to tetraploid range in the other two. Some PI tumors were diploid, but most were aneuploid (8 of 12 tumors). The most frequent alterations found in PD and PI tumors were gains of chromosomes 3, 4, and 6 and losses of chromosomes 16 and X. Chromosomes 4 and 7 were involved in translocations in three of the four tumor families studied. single-strand conformation polymorphism analysis revealed a point mutation on the Trp53 gene in one of the PD tumors; this showed a stable diploid karyotype, suggesting that mutated Trp53 is not uniquely involved in chromosome instability. We have shown that hormone independence may be acquired without changes in ploidy, suggesting that the increase in ploidy is favored by successive transplantation. In our model, diploid tumors responded to hormone treatment but aneuploid tumors were either responsive or not. © 2005 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Cytogenetic findings, Trp53 mutations, and hormone responsiveness in a medroxyprogesterone acetate induced murine breast cancer model
Autor:Fabris, V.T.; Benavides, F.; Conti, C.; Merani, S.; Lanari, C.
Filiación:Laboratorio de Carcinogénesis Hormonal, Instituto de Biología Y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Department of Carcinogenesis, University of Texas, M. D. Anderson Cancer Center, Smithville, TX, United States
Centro de Investigaciones en Reproducción (CIR), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:estrogen receptor; gestagen; medroxyprogesterone acetate; progesterone receptor; aneuploidy; animal cell; animal model; animal tissue; article; breast cancer; cancer growth; cancer transplantation; chromosomal instability; chromosome 16; chromosome 3; chromosome 4; chromosome 6; chromosome 7; chromosome mutation; chromosome number; chromosome translocation; controlled study; cytogenetics; diploidy; female; gene mutation; hormone response; mouse; mouse strain; nonhuman; point mutation; priority journal; single strand conformation polymorphism; tetraploidy; triploidy; tumor; X chromosome; Aneuploidy; Animals; Chromosome Banding; Female; Genes, p53; In Situ Hybridization, Fluorescence; Mammary Neoplasms, Experimental; Medroxyprogesterone 17-Acetate; Mice; Mice, Inbred BALB C; Neoplasms, Hormone-Dependent; Point Mutation; Tryptophan; Murinae
Año:2005
Volumen:161
Número:2
Página de inicio:130
Página de fin:139
DOI: http://dx.doi.org/10.1016/j.cancergencyto.2005.02.008
Título revista:Cancer Genetics and Cytogenetics
Título revista abreviado:Cancer Genet. Cytogenet.
ISSN:01654608
CODEN:CGCYD
CAS:medroxyprogesterone acetate, 71-58-9; Medroxyprogesterone 17-Acetate, 71-58-9; Tryptophan, 73-22-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01654608_v161_n2_p130_Fabris

Referencias:

  • Fuqua, S.A., Fitzgerald, S.D., Allred, D.C., Elledge, R.M., Nawaz, Z., McDonnell, D.P., O'Malley, B.W., McGuire, W.L., Inhibition of estrogen receptor action by a naturally occurring variant in human breast tumors (1992) Cancer Res, 52, pp. 483-486
  • Wiebe, V.J., Osborne, C.K., McGuire, W.L., Degregorio, M.W., Identification of estrogenic tamoxifen metabolite(s) in tamoxifen-resistant human breast tumors (1992) J Clin Oncol, 10, pp. 990-994
  • Santen, R., Jeng, M.H., Wang, J.P., Song, R., Masamura, S., McPherson, R., Santner, S., Shim, W.S., Adaptive hypersensitivity to estradiol: Potential mechanism for secondary hormonal responses in breast cancer patients (2001) J Steroid Biochem Mol Biol, 79, pp. 115-125
  • Osborne, C.K., Bardou, V., Hopp, T.A., Chamness, G.C., Hilsenbeck, S.G., Fuqua, S.A., Wong, J., Schiff, R., Role of the estrogen receptor coactivator AIB1 (SRC-3) and HER-2/neu in tamoxifen resistance in breast cancer (2003) J Natl Cancer Inst, 95, pp. 353-361
  • Aronica, S.M., Katzenellenbogen, B.S., Progesterone receptor regulation in uterine cells: Stimulation by estrogen, cyclic adenosine 3′, 5′- monophosphate, and insulin-like growth factor I and suppression by antiestrogens and protein kinase inhibitors (1991) Endocrinology, 128, pp. 2045-2052
  • Osborne, C.K., Schiff, R., Growth factor receptor cross-talk with estrogen receptor as a mechanism for tamoxifen resistance in breast cancer (2003) Breast, 12, pp. 362-367
  • Shou, J., Massarweh, S., Osborne, C.K., Wakeling, A.E., Ali, S., Weiss, H., Schiff, R., Mechanisms of tamoxifen resistance: Increased estrogen receptor-HER2/neu cross-talk in ER/HER2-positive breast cancer (2004) J Natl Cancer Inst, 96, pp. 926-935
  • Sandberg, A.A., Turc-Carel, C., The cytogenetics of solid tumors: Relation to diagnosis, classification and pathology (1987) Cancer, 59, pp. 387-395
  • Greaves, M.F., Biological models for leukaemia and lymphoma (2004) IARC Sci Publ, pp. 351-372
  • Dutrillaux, B., Gerbault-Seureau, M., Remvikos, Y., Zafrani, B., Prieur, M., Breast cancer genetic evolution: I. Data from cytogenetics and DNA content (1991) Breast Cancer Res Treat, 19, pp. 245-255
  • Pandis, N., Jin, Y., Gorunova, L., Petersson, C., Bardi, G., Idvall, I., Johansson, B., Mitelman, F., Chromosome analysis of 97 primary breast carcinomas: Identification of eight karyotypic subgroups (1995) Genes Chromosomes Cancer, 12, pp. 173-185
  • Steinarsdottir, M., Petursdottir, I., Snorradottir, S., Eyfjord, J.E., Ogmundsdottir, H.M., Cytogenetic studies of breast carcinomas: Different karyotypic profiles detected by direct harvesting and short-term culture (1995) Genes Chromosomes Cancer, 13, pp. 239-248
  • Tirkkonen, M., Tanner, M., Karhu, R., Kallioniemi, A., Isola, J., Kallioniemi, O.P., Molecular cytogenetics of primary breast cancer by CGH (1998) Genes Chromosomes Cancer, 21, pp. 177-184
  • Chang, J., Powles, T.J., Allred, D.C., Ashley, S.E., Makris, A., Gregory, R.K., Osborne, C.K., Dowsett, M., Prediction of clinical outcome from primary tamoxifen by expression of biologic markers in breast cancer patients (2000) Clin Cancer Res, 6, pp. 616-621
  • O'Reilly, S.M., Camplejohn, R.S., Rubens, R.D., Richards, M.A., DNA flow cytometry and response to preoperative chemotherapy for primary breast cancer (1992) Eur J Cancer, 28, pp. 681-683
  • Lanari, C., Molinolo, A.A., Pasqualini, C.D., Induction of mammary adenocarcinomas by medroxyprogesterone acetate in BALB/c female mice (1986) Cancer Lett, 33, pp. 215-223
  • Molinolo, A.A., Lanari, C., Charreau, E.H., Sanjuan, N., Pasqualini, C.D., Mouse mammary tumors induced by medroxyprogesterone acetate: Immunohistochemistry and hormonal receptors (1987) J Natl Cancer Inst, 79, pp. 1341-1350
  • Lamb, C., Simian, M., Molinolo, A., Pazos, P., Lanari, C., Regulation of cell growth of a progestin-dependent murine mammary carcinoma in vitro: Progesterone receptor involvement in serum or growth factor-induced cell proliferation (1999) J Steroid Biochem Mol Biol, 70, pp. 133-142
  • Kordon, E., Lanari, C., Meiss, R., Charreau, E., Pasqualini, C.D., Hormone dependence of a mouse mammary tumor line induced in vivo by medroxyprogesterone acetate (1990) Breast Cancer Res Treat, 17, pp. 33-43
  • Kordon, E.C., Guerra, F., Molinolo, A.A., Charreau, E.H., Pasqualini, C.D., Pazos, P., Dran, G., Lanari, C., Effect of sialoadenectomy on medroxyprogesterone-acetate-induced mammary carcinogenesis in BALB/c mice: Correlation between histology and epidermal-growth-factor receptor content (1994) Int J Cancer, 59, pp. 196-203
  • Helguero, L.A., Viegas, M., Asaithamby, A., Shyamala, G., Lanari, C., Molinolo, A.A., Progesterone receptor expression in medroxyprogesterone acetate-induced murine mammary carcinomas and response to endocrine treatment (2003) Breast Cancer Res Treat, 79, pp. 379-390
  • Lanari, C., Kordon, E., Molinolo, A., Pasqualini, C.D., Charreau, E.H., Mammary adenocarcinomas induced by medroxyprogesterone acetate: Hormone dependence and EGF receptors of BALB/c in vivo sublines (1989) Int J Cancer, 43, pp. 845-850
  • Pandis, N., Heim, S., Bardi, G., Limon, J., Mandahl, N., Mitelman, F., Improved technique for short-term culture and cytogenetic analysis of human breast cancer (1992) Genes Chromosomes Cancer, 5, pp. 14-20
  • Dran, G., Luthy, I.A., Molinolo, A.A., Charreau, E.H., Pasqualini, C.D., Lanari, C., Effect of medroxyprogesterone acetate (MPA) and serum factors on cell proliferation in primary cultures of an MPA-induced mammary adenocarcinoma (1995) Breast Cancer Res Treat, 35, pp. 173-186
  • Seabright, M., A rapid banding technique for human chromosomes (1971) Lancet, 2, pp. 971-972
  • Shen, J., Bao, Y., Liu, H.M., Lee, P., Leonard, J.V., Chen, Y.T., Mutations in exon 3 of the glycogen debranching enzyme gene are associated with glycogen storage disease type III that is differentially expressed in liver and muscle (1996) J Clin Invest, 98, pp. 352-357
  • Fabris, V., Lamb, C.A., Keck, C., Aldaz, M.C., Merani, S., Lanari, C., Karyotypic evolution of four novel mouse mammary carcinoma cell lines: Identification of marker chromosomes by fluorescence in situ hybridization (2003) Cancer Genet Cytogenet, 142, pp. 36-45
  • Efeyan, A., Fabris, V., Merani, S., Lanari, C., Molinolo, A.A., Establishment of two hormone-responsive mouse mammary carcinoma cell lines derived from a metastatic mammary tumor (2004) Breast Cancer Res Treat, 83, pp. 233-244
  • Donehower, L.A., Godley, L.A., Aldaz, C.M., Pyle, R., Shi, Y.P., Pinkel, D., Gray, J., Varmus, H.E., The role of p53 loss in genomic instability and tumor progression in a murine mammary cancer model (1996) Prog Clin Biol Res, 395, pp. 1-11
  • Li, J.J., Papa, D., Davis, M.F., Weroha, S.J., Aldaz, C.M., El Bayoumy, K., Ballenger, J., Li, S.A., Ploidy differences between hormone- and chemical carcinogen-induced rat mammary neoplasms: Comparison to invasive human ductal breast cancer (2002) Mol Carcinog, 33, pp. 56-65
  • Le Roy, H., Ricoul, M., Ogata, H., Apiou, F., Dutrillaux, B., Chromosome anomalies in mammary carcinoma from transgenic WAPRAS mice: Comparison with human data (1993) Genes Chromosomes Cancer, 6, pp. 156-160
  • Montagna, C., Andrechek, E.R., Padilla-Nash, H., Muller, W.J., Ried, T., Centrosome abnormalities, recurring deletions of chromosome 4, and genomic amplification of HER2/neu define mouse mammary gland adenocarcinomas induced by mutant HER2/neu (2002) Oncogene, 21, pp. 890-898
  • Weaver, Z.A., McCormack, S.J., Liyanage, M., Du Manoir, S., Coleman, A., Schrock, E., Dickson, R.B., Ried, T., A recurring pattern of chromosomal aberrations in mammary gland tumors of MMTV-cmyc transgenic mice (1999) Genes Chromosomes Cancer, 25, pp. 251-260
  • Pati, D., Haddad, B.R., Haegele, A., Thompson, H., Kittrell, F.S., Shepard, A., Montagna, C., Medina, D., Hormone-induced chromosomal instability in p53-null mammary epithelium (2004) Cancer Res, 64, pp. 5608-5616
  • Katayama, H., Brinkley, W.R., Sen, S., The Aurora kinases: Role in cell transformation and tumorigenesis (2003) Cancer Metastasis Rev, 22, pp. 451-464
  • Goepfert, T.M., McCarthy, M., Kittrell, F.S., Stephens, C., Ullrich, R.L., Brinkley, B.R., Medina, D., Progesterone facilitates chromosome instability (aneuploidy) in p53 null normal mammary epithelial cells (2000) FASEB J, 14, pp. 2221-2229
  • Medina, D., Kittrell, F.S., Shepard, A., Contreras, A., Rosen, J.M., Lydon, J., Hormone dependence in premalignant mammary progression (2003) Cancer Res, 63, pp. 1067-1072
  • Balana, M.E., Lupu, R., Labriola, L., Charreau, E.H., Elizalde, P.V., Interactions between progestins and heregulin (HRG) signaling pathways: HRG acts as mediator of progestins proliferative effects in mouse mammary adenocarcinomas (1999) Oncogene, 18, pp. 6370-6379
  • Montecchia, M.F., Lamb, C., Molinolo, A.A., Luthy, I.A., Pazos, P., Charreau, E., Vanzulli, S., Lanari, C., Progesterone receptor involvement in independent tumor growth in MPA-induced murine mammary adenocarcinomas (1999) J Steroid Biochem Mol Biol, 68, pp. 11-21
  • Lanari, C., Montecchia, M.F., Pazos, P., Simian, M., Vanzulli, S., Lamb, C., Molinolo, A.A., Progestin-induced mammary adenocarcinomas in BALB/c mice: Progression from hormone-dependent to autonomous tumors (1997) Medicina (B Aires), 57, pp. 55-69
  • Wang, X., Jin, D.Y., Ng, R.W., Feng, H., Wong, Y.C., Cheung, A.L., Tsao, S.W., Significance of MAD2 expression to mitotic checkpoint control in ovarian cancer cells (2002) Cancer Res, 62, pp. 1662-1668
  • Warner, S.L., Bearss, D.J., Han, H., Von Hoff, D.D., Targeting Aurora-2 kinase in cancer (2003) Mol Cancer Ther, 2, pp. 589-595
  • Aldaz, C.M., Liao, Q.Y., Paladugu, A., Rehm, S., Wang, H., Allelotypic and cytogenetic characterization of chemically induced mouse mammary tumors: High frequency of chromosome 4 loss of heterozygosity at advanced stages of progression (1996) Mol Carcinog, 17, pp. 126-133
  • Cool, M., Jolicoeur, P., Elevated frequency of loss of heterozygosity in mammary tumors arising in mouse mammary tumor virus/neu transgenic mice (1999) Cancer Res, 59, pp. 2438-2444
  • Radany, E.H., Hong, K., Kesharvarzi, S., Lander, E.S., Bishop, J.M., Mouse mammary tumor virus/v-Ha-ras transgene-induced mammary tumors exhibit strain-specific allelic loss on mouse chromosome 4 (1997) Proc Natl Acad Sci U S a, 94, pp. 8664-8669
  • Bieche, I., Khodja, A., Lidereau, R., Deletion mapping of chromosomal region 1p32-pter in primary breast cancer (1999) Genes Chromosomes Cancer, 24, pp. 255-263
  • Larramendy, M.L., Lushnikova, T., Bjorkqvist, A.M., Wistuba, I.I., Virmani, A.K., Shivapurkar, N., Gazdar, A.F., Knuutila, S., Comparative genomic hybridization reveals complex genetic changes in primary breast cancer tumors and their cell lines (2000) Cancer Genet Cytogenet, 119, pp. 132-138

Citas:

---------- APA ----------
Fabris, V.T., Benavides, F., Conti, C., Merani, S. & Lanari, C. (2005) . Cytogenetic findings, Trp53 mutations, and hormone responsiveness in a medroxyprogesterone acetate induced murine breast cancer model. Cancer Genetics and Cytogenetics, 161(2), 130-139.
http://dx.doi.org/10.1016/j.cancergencyto.2005.02.008
---------- CHICAGO ----------
Fabris, V.T., Benavides, F., Conti, C., Merani, S., Lanari, C. "Cytogenetic findings, Trp53 mutations, and hormone responsiveness in a medroxyprogesterone acetate induced murine breast cancer model" . Cancer Genetics and Cytogenetics 161, no. 2 (2005) : 130-139.
http://dx.doi.org/10.1016/j.cancergencyto.2005.02.008
---------- MLA ----------
Fabris, V.T., Benavides, F., Conti, C., Merani, S., Lanari, C. "Cytogenetic findings, Trp53 mutations, and hormone responsiveness in a medroxyprogesterone acetate induced murine breast cancer model" . Cancer Genetics and Cytogenetics, vol. 161, no. 2, 2005, pp. 130-139.
http://dx.doi.org/10.1016/j.cancergencyto.2005.02.008
---------- VANCOUVER ----------
Fabris, V.T., Benavides, F., Conti, C., Merani, S., Lanari, C. Cytogenetic findings, Trp53 mutations, and hormone responsiveness in a medroxyprogesterone acetate induced murine breast cancer model. Cancer Genet. Cytogenet. 2005;161(2):130-139.
http://dx.doi.org/10.1016/j.cancergencyto.2005.02.008