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Abstract:

The release of luteinizing hormone (LH) evoked by exogenous serotonin (5 mg/kg, i.p.) was measured at different developmental ages in the female rat. Since LH response to serotonin is modulated by estrogen, moxestrol, a synthetic compound which does not bind to α-fetoprotein, was administered 48 h before serotonin or saline injections. Serotonin was ineffective in releasing LH in 5-day females pretreated with moxestrol; the response increased abruptly at 12 and 18 days and from then onwards decreased gradually. This is in clear contrast to the development of the serotonin-induced prolactin release, which is absent in the first postnatal week, becomes evident on day 12 and increases gradually as the rat approaches puberty. In a second group of experiments, the ontogenesis of hypothalamic-preoptic suprachiasmatic [3H]serotonin binding was studied in 5-, 16-, 27- and 37-day-old female rats. A specific serotonergic binding site could be quantified at 5 days (Bmax 4.31 : 1.89 fmol/μg DNA; Kd:2.74 : 1.17 nM). A gradual increase of both, Bmax and Kd was observed as the animal matured: the number of binding sites almost doubled from day 16 to day 27, and increased even further by 37 days (Bmax 24.21 : 5.16 fmol/μg DNA). These data indicate: (a) a specific [3H]serotonin binding site in hypothalamic-preoptic suprachiasmatic area is present in the first postnatal week of the female rat: (b) Bmax and Kd values increase from 5 to 37 days of age; (c) serotonin-induced LH release in moxestrol-pretreated females is maximal in postnatal weeks 2-3; and (d) ontogenesis of serotonin binding sites in the hypothalamus is in correlation to prolactin, but not to LH release evoked by exogenously administered indoleamine. © 1986.

Registro:

Documento: Artículo
Título:Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats
Autor:Becú-Villalobos, D.; Libertun, C.
Filiación:Instituto de Biología y Medicina Experimental, 1428 Buenos Aires, Argentina
Palabras clave:hypothalamic-preoptic suprachiasmatic area; luteinizing hormone (LH); moxestrol; ontogenesis; prolactin; [3H]serotonin binding; indoleamine; moxestrol; prolactin; radioisotope; animal experiment; autonomic nervous system; autopsy; central nervous system; drug binding; drug receptor binding; etiology; hypothalamus; intraperitoneal drug administration; luteinizing hormone release; methodology; nonhuman; pharmacokinetics; preoptic area; priority journal; rat; serotonin h 3; subcutaneous drug administration; suprachiasmatic nucleus
Año:1986
Volumen:25
Número:1
Página de inicio:111
Página de fin:116
DOI: http://dx.doi.org/10.1016/0165-3806(86)90157-4
Título revista:Developmental Brain Research
Título revista abreviado:Dev. Brain Res.
ISSN:01653806
CODEN:DBRRD
CAS:indoleamine, 56480-48-9; moxestrol, 34816-55-2; prolactin, 12585-34-1, 50647-00-2, 9002-62-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01653806_v25_n1_p111_BecuVillalobos

Referencias:

  • Becú, Libertun, Comparative maturation of the regulation of prolactin and thyrotropin by serotonin and thyrotropin-releasing hormone in male and female rats (1982) Endocrinology, 110, pp. 1879-1883
  • Becú-Villalobos, Lux, Lacau de Mengido, Libertun, Sexual differences in the serotonergic control of prolactin and luteinizing hormone secretion in the rat (1984) Endocrinology, 115, pp. 84-89
  • Becú-Villalobos, Vacas, Cardinali, Libertun, A developmental study of adenohypophyseal dopaminergic receptors and of haloperidol-induced prolactin release in rats (1984) Dev. Brain Res., 12, pp. 167-171
  • Biegon, Bercovitz, Samuel, Serotonin receptor concentration during the estrous cycle of the rat (1980) Brain Res., 187, pp. 221-225
  • Blank, Panerai, Friesen, Opioid peptides modulate luteinizing hormone secretion during sexual maturation (1979) Science, 203, pp. 1129-1131
  • Burton, A study of conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid (1951) Biochem. J., 6, pp. 315-317
  • Chen, Meites, Effects of biogenic amines and TRH on the release of prolactin and TSH in the rat (1975) Endocrinology, 96, pp. 10-14
  • Chen, Sylvester, Ieri, Meites, Potentiation of luteinizing hormone release by serotonin agonists in ovariectomized steroid-primed rats (1981) Endocrinology, 108, pp. 948-952
  • Cresie, Keightley, The underlying structure of the direct linear plot with application to the analysis of hormone receptor interactions (1979) J. Steroid Biochem., 11, pp. 1173-1180
  • Dudley, Prepubertal ontogeny of responsiveness to estradiol in female rat central nervous system (1981) Neurosci. Behav. Rev., 5, pp. 421-435
  • Fillion, Central serotonin receptors: regulation mechanism at the molecular level (1983) Molecular Pharmacology of Neurotransmitter Receptors, pp. 115-123. , T. Segawa, H.I. Yamamura, K. Kuriyama, Raven, New York
  • Fillion, Beaudoin, Rousselle, Jacob, [3H]5-HT binding sites and 5-HT-sensitive adenylate cyclase in glial cell membrane fraction (1980) Brain Res., 198, pp. 361-375
  • Héry, Laplante, Kordon, Participation of serotonin in the phasic release of LH. I. Evidence from pharmacological experiments (1976) Endocrinology, 99, pp. 496-505
  • Ieri, Chen, Meites, Effects of morphine and naloxone on serum levels of luteinizing hormone and prolactin in prepubertal male and female rats (1979) Neuroendocrinology, 29, pp. 288-292
  • Johnson, Crowley, Acute effects of estradiol on circulating luteinizing hormone and prolactin concentrations and on serotonin turnover in individual brain nuclei (1983) Endocrinology, 113, pp. 1935-1941
  • Kato, Serotonin content of rat brain in relation to sex and age (1960) J. Neurochem., 5, p. 202
  • Kirksey, Slotkin, Concomitant development of [3H]dopamine and [3H]hydroxytryptamine uptake systems in rat brain region (1979) Br. J. Pharmacol., 67, pp. 387-391
  • Lawson, Gala, The influence of pharmacological manipulation of serotonergic and dopaminergic mechanisms on plasma prolactin in ovariectomized, estrogen-treated rats (1978) Endocrinology, 102, pp. 973-981
  • Lidov, Molliver, An immunohistochemical study of serotonin neuron development in the rat: ascending pathways and terminal fields (1982) Brain Res. Bull., 8, pp. 389-430
  • Lidov, Molliver, Immunohistochemical study of the development of serotonergic neurons in the rat CNS (1982) Brain Res. Bull., 9, pp. 559-604
  • Maggi, U'Prichard, Enna, Differential effects of antidepressant treatment on brain monoaminergic receptors (1980) Eur. J. Pharmacol., 61, pp. 91-98
  • MacKinnon, Mattock, Ter Haar, Serum gonadotrophin levels during development in male, female and androgenized female rats and the effect of general disturbance of high luteinizing hormone levels (1976) J. Endocrinol., 70, pp. 361-371
  • Moguilevsky, Faigón, Scacchi, Szwarcfarb, Effect of serotoninergic system on luteinizing hormone secretion in prepubertal female rats (1985) Neuroendocrinology, 40, pp. 135-138
  • Nelson, Herbet, Bourgoin, Glowinski, Hamon, Characteristics of central 5-HT receptors and their adaptive changes following intracerebral 5,7-dihydroxytryptamine administration in the rat (1978) Mol. Pharmacol., 14, pp. 983-995
  • Nelson, Herbet, Enjalbert, Bockaert, Hamon, Serotonin-sensitive adenylate cyclase and [3H]-serotonin binding sites in the CNS of the rat. I and II (1980) Biochem. Pharmcol., 29, pp. 2445-2453
  • Ness, Ruzsas, Role of the serotoninergic neuron system of the brain stem on the release of thyrotropic and luteinizing hormone (1981) J. Physiol. (London), 77, pp. 501-503
  • Ojeda, Jameson, McCann, Developmental changes in pituitary responsiveness to luteinizing hormone-releasing hormone (LH-RH) in the female rat: ovarian-adrenal influence during the infantile period (1977) Endocrinology, 100, pp. 440-451
  • Peroutka, Snyder, Multiple serotonin receptors: differential binding of [3H]5-hydroxytryptamine, [3H]lysergic acid diethylamide and [3H]spiroperidol (1979) Mol. Pharmacol., 16, pp. 687-699
  • Raynaud, Influence of rat estradiol binding plasma protein (EBP) on uterotrophic activity (1973) Steroids, 21, pp. 249-258
  • Raynaud, Moguilewsky, Vannier, Influence of rat estradiol binding plasma protein (EBP) on estrogen binding to its receptor and on induced biological responses (1979) Advances in the Biosciences, 25, pp. 59-75. , A.M. Kaye, M. Kaye, Pergamon, Oxford
  • Savage, Frazer, Mendels, Differential effects of monoamine oxidase inhibitors and serotonin reuptake inhibitors on [3H]-serotonin receptor binding in rat brain (1979) Eur. J. Pharmacol., 58, pp. 87-88
  • Schneider, McCann, Mono- and indoleamines and control of LH secretion (1970) Endocrinology, 86, pp. 1127-1133
  • Steigrad, Tobler, Waser, Borbely, Effect of p-chlorophenylalanine on cerebral serotonin binding, serotonin concentration and motor activity in the rat (1978) Naunyn-Schmiedebergs Arch. Pharmacol., 305, pp. 143-148
  • Watts, Stanley, Indoleamines in the hypothalamus and area of the midbrain raphe nuclei of male and female rats throughout postnatal development (1984) Neuroendocrinology, 38, pp. 461-466
  • Weiner, Ganong, Role of brain monoamines and histamine in regulation of anterior pituitary secretion (1978) Physiol. Rev., 58, pp. 905-976
  • Wuttke, Honma, Lamberts, Hohn, The role of monoamines in female puberty (1980) Fed. Proc. Fed. Am. Soc. Exp. Biol., 39, pp. 2378-2383
  • Zeisel, Mauron, Watkins, Wurtman, Developmental changes in brain indoles, serum tryptophan and other serum neutral amino acids in the rat (1981) Dev. Brain Res., 1, pp. 551-564

Citas:

---------- APA ----------
Becú-Villalobos, D. & Libertun, C. (1986) . Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats. Developmental Brain Research, 25(1), 111-116.
http://dx.doi.org/10.1016/0165-3806(86)90157-4
---------- CHICAGO ----------
Becú-Villalobos, D., Libertun, C. "Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats" . Developmental Brain Research 25, no. 1 (1986) : 111-116.
http://dx.doi.org/10.1016/0165-3806(86)90157-4
---------- MLA ----------
Becú-Villalobos, D., Libertun, C. "Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats" . Developmental Brain Research, vol. 25, no. 1, 1986, pp. 111-116.
http://dx.doi.org/10.1016/0165-3806(86)90157-4
---------- VANCOUVER ----------
Becú-Villalobos, D., Libertun, C. Ontogenesis of [3H]serotonin binding sites in the hypothalamus of the female rat: Relation to serotonin-induced LH release in moxestrol-pretreated rats. Dev. Brain Res. 1986;25(1):111-116.
http://dx.doi.org/10.1016/0165-3806(86)90157-4