Artículo

Gamba, G.; Bonorino, P.; Gonzalez-Videla, R.; Benedetti, R.; Cavalieri, H.; Courreges, M.C.; Massouh, E.J.; Benencia, F. "Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route" (2004) Immunology Letters. 92(3):245-252
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Abstract:

Here, we studied the effect of aminoguanidine (AG) treatment, a nitric oxide synthase (NOS)-2 inhibitor, during the immune response against intranasal administration of ovalbumin (OVA) mixed with cholera toxin (CT) in BALB/c mice. NOS-2 mRNA was detected by reverse transcription-PCR (RT-PCR) in samples of lungs and turbinates early post-inoculation of the antigen. Animals intranasally treated with AG, showed an increase in the levels of seric specific IgG and IgM. A higher IgG1/IgG2a ratio against OVA was also observed in sera of same animals. Moreover, high levels of specific IgA were detected in samples of pulmonar washings obtained from treated animals. On the contrary, treated animals showed a lower DTH response while splenocytes obtained from the same animals showed a reduced proliferative capability against OVA compared to controls. Finally, RT-PCR analysis showed increased expression of TGF-β in turbinates, lungs and cells from pulmonar washings obtained from AG treated mice. Taken together, these data suggest a role of nitric oxide (NO) in modulating the primary immune response against intranasal antigens. © 2004 Published by Elsevier B.V.

Registro:

Documento: Artículo
Título:Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route
Autor:Gamba, G.; Bonorino, P.; Gonzalez-Videla, R.; Benedetti, R.; Cavalieri, H.; Courreges, M.C.; Massouh, E.J.; Benencia, F.
Filiación:Department of Biological Chemistry, University of Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Member Consejo Nac. de Invest. Cie., Argentina
Palabras clave:Cholera toxin; Mucosal immunity; Nitric oxide; aminoguanidine; cholera toxin; immunoglobulin A; immunoglobulin G; immunoglobulin G1; immunoglobulin G2a; immunoglobulin M; nitric oxide; nitric oxide synthase inhibitor; ovalbumin; transforming growth factor beta; analytic method; animal cell; antigen specificity; article; controlled study; drug effect; drug inhibition; immune response; inoculation; male; mouse; mouse strain; mucosal immunity; nonhuman; priority journal; reverse transcription polymerase chain reaction; sampling; spleen cell; Administration, Intranasal; Animals; Cholera Toxin; Guanidines; Immunity, Cellular; Immunity, Mucosal; Lung; Mice; Nitric Oxide; Nitric Oxide Synthase; Ovalbumin; Transforming Growth Factor beta; Turbinates
Año:2004
Volumen:92
Número:3
Página de inicio:245
Página de fin:252
DOI: http://dx.doi.org/10.1016/j.imlet.2004.01.003
Título revista:Immunology Letters
Título revista abreviado:Immunol. Lett.
ISSN:01652478
CODEN:IMLED
CAS:aminoguanidine, 1068-42-4, 2582-30-1, 79-17-4; immunoglobulin G, 97794-27-9; immunoglobulin M, 9007-85-6; nitric oxide, 10102-43-9; ovalbumin, 77466-29-6; Cholera Toxin, 9012-63-9; Guanidines; Nitric Oxide Synthase, EC 1.14.13.39; Nitric Oxide, 10102-43-9; Ovalbumin, 9006-59-1; pimagedine, 79-17-4; Transforming Growth Factor beta
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v92_n3_p245_Gamba

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Citas:

---------- APA ----------
Gamba, G., Bonorino, P., Gonzalez-Videla, R., Benedetti, R., Cavalieri, H., Courreges, M.C., Massouh, E.J.,..., Benencia, F. (2004) . Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route. Immunology Letters, 92(3), 245-252.
http://dx.doi.org/10.1016/j.imlet.2004.01.003
---------- CHICAGO ----------
Gamba, G., Bonorino, P., Gonzalez-Videla, R., Benedetti, R., Cavalieri, H., Courreges, M.C., et al. "Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route" . Immunology Letters 92, no. 3 (2004) : 245-252.
http://dx.doi.org/10.1016/j.imlet.2004.01.003
---------- MLA ----------
Gamba, G., Bonorino, P., Gonzalez-Videla, R., Benedetti, R., Cavalieri, H., Courreges, M.C., et al. "Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route" . Immunology Letters, vol. 92, no. 3, 2004, pp. 245-252.
http://dx.doi.org/10.1016/j.imlet.2004.01.003
---------- VANCOUVER ----------
Gamba, G., Bonorino, P., Gonzalez-Videla, R., Benedetti, R., Cavalieri, H., Courreges, M.C., et al. Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route. Immunol. Lett. 2004;92(3):245-252.
http://dx.doi.org/10.1016/j.imlet.2004.01.003