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Abstract:

The ability of peripheral mononuclear cells from Chagas' disease patients (CDP) to generate specific proliferative responses in vitro to whole homogenate (WH) and subcellular fractions of T. cruzi was investigated. Flagellar (F), microsomal (Mc) and cell sap (CS) fractions were isolated from a WH obtained by disruption by pressure-depressure. Parasite-free human peripheral mononuclear cells were isolated by centrifugation of leukocyte-rich plasma over discontinuous gradients of Ficoll-Hypaque and cultured for 6 days in the presence of the different fractions of T. cruzi. Eighty-six percent ( 54 63) of the CDP showed good capacity to respond to at least one of the fractions assayed while only 18% ( 4 22) of the controls reacted. The Mc fraction showed the best specificity as well as the maximal stimulating effect in CDP ( 37 55 vs 0 18 in controls) while WH (62% vs 10%), F (54% vs 16%) and CS (52% vs 6%) showed a lesser antigenic capacity. These results suggest that recognition mechanisms and proliferative responses are functional in CDP. No correlation was found between the stimulating capacity of fractions in vitro and their immunogenic capacity previously observed in vivo. © 1983.

Registro:

Documento: Artículo
Título:Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi
Autor:De Titto, E.H.; Segura, E.L.; Braun, M.
Filiación:Departamento de Immunofarmacología, Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Serrano 665, 1414 Buenos Aires, Argentina
Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas Dr. Mario Fatala Chaben (INDIECH), Ministerio de Salud Pública y Medio Ambiente, Av. Paseo Colón 568, (1063) Buenos Aires, Argentina
Palabras clave:cellular immunity; Chagas' disease; lymphoproliferative response; specific response in Chagas' disease; subcellular fractions of T. cruzi; Trypanosoma cruzi; blood and hemopoietic system; cellular immunity; chagas disease; clinical article; diagnosis; etiology; human; protozoon; trypanosoma cruzi; Cell Count; Cell Separation; Chagas Disease; Human; Immunity, Cellular; Kinetics; Lymphocyte Activation; Lymphocytes; Phytohemagglutinins; Subcellular Fractions; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1983
Volumen:6
Número:3
Página de inicio:161
Página de fin:167
DOI: http://dx.doi.org/10.1016/0165-2478(83)90099-8
Título revista:Immunology Letters
Título revista abreviado:Immunol. Lett.
ISSN:01652478
CODEN:IMLED
CAS:Phytohemagglutinins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v6_n3_p161_DeTitto

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Citas:

---------- APA ----------
De Titto, E.H., Segura, E.L. & Braun, M. (1983) . Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi. Immunology Letters, 6(3), 161-167.
http://dx.doi.org/10.1016/0165-2478(83)90099-8
---------- CHICAGO ----------
De Titto, E.H., Segura, E.L., Braun, M. "Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi" . Immunology Letters 6, no. 3 (1983) : 161-167.
http://dx.doi.org/10.1016/0165-2478(83)90099-8
---------- MLA ----------
De Titto, E.H., Segura, E.L., Braun, M. "Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi" . Immunology Letters, vol. 6, no. 3, 1983, pp. 161-167.
http://dx.doi.org/10.1016/0165-2478(83)90099-8
---------- VANCOUVER ----------
De Titto, E.H., Segura, E.L., Braun, M. Cellular immunity in chagas' disease patients. Lymphoproliferative response to subcellular fractions of Trypanosoma cruzi. Immunol. Lett. 1983;6(3):161-167.
http://dx.doi.org/10.1016/0165-2478(83)90099-8